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The Sumo protease Senp7 is required for proper neuronal differentiation
Open accessAbstract
Covalent attachment of the Small ubiquitin-like modifier (Sumo) polypeptide to proteins regulates many processes in the eukaryotic cell. In the nervous system, Sumo has been associated with the synapsis and with neurodegenerative diseases. However, its involvement in regulating neuronal differentiation remains largely unknown. Here we show that net Sumo deconjugation is observed during neurogenesis and that Sumo overexpression impairs this process. In an attempt to shed light on the underlying mechanisms, we have analyzed the expression profile of genes coding for components of the sumoylation pathway following induction of neuronal differentiation. Interestingly, we observed strong upregulation of the Senp7 protease at both mRNA and protein levels under differentiation conditions. Sumo proteases, by removing Sumo from targets, are key regulators of sumoylation. Strikingly, loss-of-function analysis demonstrated that Senp7 is required for neuronal differentiation not only in a model cell line, but also in the developing neural tube. Finally, reporter-based analysis of the Senp7 promoter indicated that Senp7 was transiently activated at early stages of neuronal differentiation. Thus, the Sumo protease Senp7 adds to the list of factors involved in vertebrate neurogenesis.
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“Furthermore, recent whole blood and cell-specific Methylome-wide association studies (MWAS) from a sample of adolescents with CU disorder pointed at many methylation sites relevant to brain function and to neurodevelopment (Clark et al., 2021). These included CpGs located in the CLMN gene and the SENP7 gene, expressed in the brain and playing a role in brain developmental and synaptic function and”
From Can epigenetics shine a light on the biological pathways underlying major mental disorders? (2022) · cites this paper as Juarez-Vicente, Luna-Pelaez, & Garcia-Dominguez, 2016
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“The SUMO protease SENP7 has been involved in neurogenesis (Juárez-Vicente et al., 2016), while sumoylation of BRAF35, a subunit of the LSD1 histone demethylase complex, assures the undifferentiated state of neural progenitors (Ceballos-Chávez et al.”
BackgroundZhang, M., & Wang, X.-L. (2022) · Functional & Integrative Genomics · doi:10.1007/s10142-022-00914-z
“2022 Apr 1), LCLAT1(Juarez-Vicente et al. 2016), and ELF2(Rieger et al.”
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Metadata
- Authors
- Juarez-Vicente, F., Luna-Pelaez, N., & Garcia-Dominguez, M.
- Venue
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
- Publisher
- Elsevier BV
- Published
- 2016-07
- Volume
- 1863
- Issue
- 7
- Pages
- 1490-1498
- ISSN
- 0167-4889 (ISSN-L)
- OpenAlex
- W2327684284
- Cited by
- 30
- References
- 0
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 1 · 2023: 2 · 2022: 8 · 2021: 5 · 2020: 3 · 2018: 2 · 2017: 3
- FWCI
- 0.85
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 365 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Biochimica et Biophysica Acta (BBA) - Molecular Cell Research (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- RISalud Institutional Health Repository of Andalucía (submittedVersion, other-oa)
- Funders
- Ministerio de Economía y Competitividad (BFU2012-37304/BFI)
- Consejo Superior de Investigaciones Científicas
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- SENP7, SUMO protease, SUMOylation, SUMO deconjugation, neuronal differentiation, neurogenesis, neural tube development, gene expression profiling, promoter activation, brain development
- MeSH terms
- Animals, Chick Embryo, Endopeptidases, Promoter Regions, Genetic, RNA, Messenger, Time Factors, Transfection, Tretinoin, Signal Transduction, Transcriptional Activation, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Developmental, Gene Expression Profiling, RNA Interference, Cell Line, Tumor, Mice, Neural Tube, Neurogenesis, Sumoylation, Neural Stem Cells
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