Nanosecond pulsed electric fields depolarize transmembrane potential via voltage-gated K+, Ca2+ and TRPM8 channels in U87 glioblastoma cells
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Nanosecond pulsed electric fields (nsPEFs) have a variety of applications in the biomedical and biotechnology industries. Cancer treatment has been at the forefront of investigations thus far as nsPEFs permeabilize cellular and intracellular membranes leading to apoptosis and necrosis. nsPEFs may also influence ion channel gating and have the potential to modulate cell physiology without poration of the membrane. This phenomenon was explored using live cell imaging and a sensitive fluorescent probe of transmembrane voltage in the human glioblastoma cell line, U87 MG, known to express a number of voltage-gated ion channels. The specific ion channels involved in the nsPEF response were screened using a membrane potential imaging approach and a combination of pharmacological antagonists and ion substitutions. It was found that a single 10ns pulsed electric field of 34kV/cm depolarizes the transmembrane potential of cells by acting on specific voltage-sensitive ion channels; namely the voltage and Ca2+ gated BK potassium channel, L- and T-type calcium channels, and the TRPM8 transient receptor potential channel.
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Electroporation pulses can range from nanoseconds to milliseconds in duration and tend to form aqueous pores in the plasma membrane that are on the order of ~1 nm in radius.
“Electroporation pulses can range from nanoseconds to milliseconds in duration and tend to form aqueous pores in the plasma membrane that are on the order of ~1 nm in radius [60,62,63,64].”
From Permeabilizing Cell Membranes with Electric Fields (2021) · cites this paper as [63]
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References 90 · Cited by 58
- Authors
- Burke, R. C., Bardet, S. M., Carr, L., Romanenko, S., Arnaud-Cormos, D., Leveque, P., & O'Connor, R. P.
- Affiliations
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- O'Connor, Rodney P.
- Venue
- Biochimica et Biophysica Acta (BBA) - Biomembranes
- Publisher
- Elsevier BV
- Published
- 2017-10
- Volume
- 1859
- Issue
- 10
- Pages
- 2040-2050
- ISSN
- 0005-2736 (ISSN-L)
- OpenAlex
- W2731378869
- Cited by
- 58
- References
- 90
- Citations by year
- 2026: 6 · 2025: 1 · 2024: 1 · 2023: 8 · 2022: 9 · 2021: 17 · 2020: 6 · 2019: 3 · 2018: 5 · 2017: 2
- FWCI
- 3.21
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biochimica et Biophysica Acta (BBA) - Biomembranes (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Région du Limousin (BiongLim)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Electromagnetic Fields and Biological Effects
- Keywords
- nanosecond pulsed electric fields, voltage-gated ion channels, BK channels, L-type calcium channels, T-type calcium channels, TRPM8, U87 glioblastoma cells, membrane potential imaging, channel gating
- MeSH terms
- Calcium, Electricity, Glioblastoma, Humans, Membrane Potentials, Necrosis, Potassium, Potassium Channels, Ion Channel Gating, Apoptosis, Calcium Channels, T-Type, Cell Line, Tumor, TRPM Cation Channels
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