Adolescent dietary manipulations differentially affect gut microbiota composition and amygdala neuroimmune gene expression in male mice in adulthood
Open accessAbstract
Adolescence is a critical developmental period that is characterised by growth spurts and specific neurobiological, neuroimmune and behavioural changes. In tandem the gut microbiota, which is a key player in the regulation of health and disease, is shaped during this time period. Diet is one of the most important regulators of microbiota composition. Thus, we hypothesised that dietary disturbances of the microbiota during this critical time window result in long-lasting changes in immunity, brain and behaviour. C57BL/6 male mice were exposed to either high fat diet or cafeteria diet during the adolescent period from postnatal day 28 to 49 and were tested for anxiety-related and social behaviour in adulthood. Our results show long-lasting effects of dietary interventions during the adolescent period on microbiota composition and the expression of genes related to neuroinflammation or neurotransmission. Interestingly, changes in myelination-related gene expression in the prefrontal cortex following high fat diet exposure were also observed. However, these effects did not translate into overt behavioural changes in adulthood. Taken together, these data highlight the importance of diet-microbiota interactions during the adolescent period in shaping specific outputs of the microbiota-gut-brain axis in later life.
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In a mouse model of adolescence, exposure to a cafeteria diet for 21 days resulted in decreased species evenness and abundance of Roseburia, Turicibacter, and Enterorhabdus, and altered gene expression involved in neuroimmunity and neurotransmission in the prefrontal cortex and amygdala, although no behavioral manifestations were observed.
“In a mouse model of adolescence, exposure to a cafeteria diet for 21 d resulted in decreased species evenness (measured by the Shannon diversity index) and abundance of Roseburia, Turicibacter, and Enterorhabdus, and, although no behavioral manifestations were observed, altered gene expression involved in neuroimmunity and neurotransmission in the prefrontal cortex and amygdala (294).”
From Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health (2021) · cites this paper as [294]
In mice, a high-fat diet decreased Shannon diversity.
“↓ Shannon diversity in HFD mice”
From Diet and the Microbiota–Gut–Brain Axis: Sowing the Seeds of Good Mental Health (2021) · cites this paper as [294]
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References 90 · Cited by 36
- Authors
- Fülling, C., Lach, G., Bastiaanssen, T. F. S., Fouhy, F., O'Donovan, A. N., Ventura-Silva, A.-P., Stanton, C., Dinan, T. G., & Cryan, J. F.
- Affiliations
- University College Cork (IE)
- Teagasc - The Irish Agriculture and Food Development Authority (IE)
- Corresponding author
- Cryan, John F.
- ORCID
- Venue
- Brain Behavior and Immunity
- Publisher
- Elsevier BV
- Published
- 2020-07
- Volume
- 87
- Pages
- 666-678
- ISSN
- 0889-1591 (ISSN-L)
- OpenAlex
- W3006993202
- Cited by
- 36
- References
- 90
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 6 · 2023: 7 · 2022: 3 · 2021: 9 · 2020: 4
- FWCI
- 1.87
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Brain Behavior and Immunity (publishedVersion, cc-by)
- PubMed (publishedVersion)
- T-Stór (Teagasc) (submittedVersion, cc-by-nc-sa)
- Funders
- Science Foundation Ireland (SFI/12/RC/2273_P2)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Neuroscience › Biological Psychiatry › Tryptophan and brain disorders
- Health Sciences › Medicine › Gastroenterology › Gastrointestinal motility and disorders
- Keywords
- adolescence, gut microbiota, high-fat diet, cafeteria diet, amygdala, microbiota-gut-brain axis, anxiety-like behavior, social behavior, myelination, prefrontal cortex, neuroinflammation, neurotransmission, diet-microbiome interactions, long-term dietary effects
- MeSH terms
- Gastrointestinal Microbiome, Amygdala, Animals, Anxiety, Male, Mice, Inbred C57BL, Gene Expression, Mice
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