CoChat
Sign Up
Cited by 31RNA Research and SplicingNuclear Structure and FunctionRNA and protein synthesis mechanisms

TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD

Saito, Kuniaki, Fujiwara, Toshinobu, Katahira, Jun, Inoue, Kunio, Sakamoto, Hiroshi·2004·Biochemical and Biophysical Research Communications
DOI 10.1016/j.bbrc.2004.06.140
Open this paper in CoChat — free account

Abstract

Hu proteins are RNA-binding proteins that are implicated in the control of stabilization, nuclear export, and/or translation of specific mRNAs with AU-rich elements (AREs) in the 3'-untranslated region. Three neuron-specific Hu proteins (HuD, HuB, and HuC), but not a ubiquitously expressed Hu protein HuR, have an activity to induce neurite outgrowth when they are overexpressed in a rat neuronal cell line PC12. Here we show that TAP/NXF1, the primary export receptor for the bulk mRNA, is a specific binding partner for HuD. In vitro binding experiments using recombinant proteins revealed that the interaction between TAP and HuD is direct and that HuD can form a ternary complex together with both TAP and RNA. Interestingly, HuR does not interact with TAP. These results suggest that HuD acts as a novel adaptor protein to recruit TAP for efficient export of ARE-containing mRNAs in neuronal cells.

Integrity signals

Registry

Crossref · 10.1016/j.bbrc.2004.06.140

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • The first two RRMs of HuD associate with the primary general mRNA export adaptor complex TAP-p15 in an RNA-independent manner, and this interaction may be necessary for shuttling of HuD.

    “Moreover, in vitro binding assays showed that the first two RRMs of HuD associate with the primary general mRNA export adaptor complex (TAP-p15; also known as NXF1/NXT1) in a RNA-independent manner and that this interaction may be necessary for shuttling of HuD (Saito et al. 2004).”

    From Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction (2013) · cites this paper as Saito et al. 2004

Cited by

Sorted by citations

Authors
Saito, K., Fujiwara, T., Katahira, J., Inoue, K., & Sakamoto, H.
Affiliations
  • Kobe University (JP)
  • The University of Osaka (JP)
Corresponding author
Sakamoto, Hiroshi
ORCID
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2004-08
Volume
321
Issue
2
Pages
291-297
ISSN
0006-291X (ISSN-L)
DOI
10.1016/j.bbrc.2004.06.140
OpenAlex
W1992240096
Cited by
31
References
0
Citations by year
2026: 2 · 2025: 1 · 2024: 1 · 2022: 2 · 2020: 1 · 2019: 1 · 2018: 1 · 2017: 3 · 2014: 1 · 2013: 2 · 2012: 2
FWCI
0.83
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Biochemical and Biophysical Research Communications (publishedVersion)
  • PubMed (publishedVersion)
Funders
Ministry of Education, Culture, Sports, Science and Technology
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA Research and Splicing
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
Keywords
HUD, HU protein, mRNA export, AU-rich elements, RNA-binding proteins, HuR, ternary complex, adaptor proteins, neurite outgrowth, neurons, PC12 cells, mRNA stabilization
MeSH terms
ELAV-Like Protein 4, Animals, Binding Sites, HeLa Cells, Humans, Nerve Tissue Proteins, Neurons, Protein Binding, RNA, Messenger, Neurites, RNA-Binding Proteins, PC12 Cells, Sequence Deletion, Protein Structure, Tertiary, Nucleocytoplasmic Transport Proteins, RNA Transport, Rats, ELAV Proteins

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review