Wnt/β-catenin signaling changes C2C12 myoblast proliferation and differentiation by inducing Id3 expression
Abstract
Canonical Wnt signaling plays important roles in regulating cell proliferation and differentiation. In this study, we report that inhibitor of differentiation (Id)3 is a Wnt-inducible gene in mouse C2C12 myoblasts. Wnt3a induced Id3 expression in a β-catenin-dependent manner. Bone morphogenetic protein (BMP) also potently induced Id3 expression. However, Wnt-induced Id3 expression occurred independent of the BMP/Smad pathway. Functional studies showed that Id3 depletion in C2C12 cells impaired Wnt3a-induced cell proliferation and alkaline phosphatase activity, an early marker of osteoblast cells. Id3 depletion elevated myogenin induction during myogenic differentiation and partially impaired Wnt3a suppressed myogenin expression in C2C12 cells. These results suggest that Id3 is an important Wnt/β-catenin induced gene in myoblast cell fate determination.
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ID3 enhances β-catenin transcriptional activity by blocking E47-mediated repression.
“Proteomic and IPA analyses also identified ID3, which enhances β‐catenin transcriptional activity by blocking E47‐mediated repression. 70 , 71”
Cites
Tanaka, S., Terada, K., & Nohno, T. (2011) · Journal of Molecular Signaling · doi:10.1186/1750-2187-6-12 · Cited by 124 · see citing works
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Gong, K., Zhou, F., Huang, H., Gong, Y., & Zhang, L. (2012) · Protein & Cell · doi:10.1007/s13238-012-2058-x · Cited by 29 · see citing works
Self-citationPineda‐Lopez, L., Aguillon, D., Villar‐Vesga, J., Valderrama‐Carmona, P., Guerrero, A., Upreti, A., Arevalo‐Alquichire, S., Correa, L., Mendez‐Castellanos, K., Castaño, D., et al. (2026) · Alzheimer's & Dementia · doi:10.1002/alz.71148 · Cited by 4 · see citing works
- Authors
- Zhang, L., Shi, S., Zhang, J., Zhou, F., & ten Dijke, P.
- Affiliations
- Leiden University Medical Center (NL)
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2012-03
- Volume
- 419
- Issue
- 1
- Pages
- 83-88
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W1990637280
- Cited by
- 21
- References
- 1
- Citations by year
- 2026: 2 · 2024: 1 · 2023: 2 · 2021: 1 · 2019: 2 · 2017: 3 · 2015: 1 · 2014: 2 · 2013: 3 · 2012: 3
- FWCI
- 1.16
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Leiden Repository (Leiden University) (submittedVersion)
- Funders
- Netherlands Organization for Scientific Research (918.66.066)
- Centre for Biomedical Genetics
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Keywords
- Wnt/β-catenin signaling, ID3, C2C12 myoblasts, Wnt3a, BMP, myoblast proliferation, myogenesis, myogenin, osteogenic differentiation, alkaline phosphatase, cell fate determination
- MeSH terms
- Alkaline Phosphatase, Animals, Cell Differentiation, Cell Line, Signal Transduction, Bone Morphogenetic Proteins, Myoblasts, NIH 3T3 Cells, Cell Proliferation, beta Catenin, Mice, Smad Proteins, Inhibitor of Differentiation Proteins, Wnt3A Protein
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