Influence of apolipoprotein A-V on hepatocyte lipid droplet formation
Open accessAbstract
Apolipoprotein A-V (apoA-V) is postulated to modulate intra-hepatic triglyceride (TG) trafficking. Stably transfected McA-RH7777 hepatocarcinoma cells expressing human apoA-V displayed enhanced neutral lipid staining while conditioned media from these cells had 40±8% less TG than cells transfected with a control vector. To obtain homogeneous cell lines expressing different amounts of apoA-V, a strategy of clonal selection was pursued. Immunoblot analysis of two distinct apoA-V stable cell lines yielded one that expresses low amounts of apoA-V and another that expresses higher amounts. Confocal fluorescence microscopy of control cells and cells expressing low levels of apoA-V had similar numbers of lipid droplets while cells expressing higher amounts of apoA-V had twice as many lipid droplets, on average. Thus, apoA-V expression promotes lipid droplet accumulation in these cells.
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APOE and APOCIII were identified on nuclear lipid droplets in HepG2 cells, and APOAV has been reported on the surface of cytoplasmic lipid droplets in adipocytes.
“APOE and APOCIII were identified on nuclear LDs in HepG2 cells, and APOAV has previously been reported on the surface of cytoplasmic LDs in adipocytes (Shu et al., 2010; Gao et al., 2012; Sołtysik et al., 2019).”
From APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size (2024) · cites this paper as Gao et al., 2012
The Discussion (page unavailable) reports apoA-V on cytosolic lipid droplets in hepatocyte-derived cells, supporting that part of the claim. It does not place apoA-V on droplets in adipocytes or report APOE and APOCIII on nuclear lipid droplets in HepG2 cells. (LLM, conf=0.88)
Experimental — accuracy not yet measuredAPOV and APOCIII have been shown to go to lipid droplets.
“APOV and APOCIII have been shown to go to LDs (Shu et al., 2010; Gao et al., 2012; Sołtysik et al., 2019).”
From APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size (2024) · cites this paper as Gao et al., 2012
The Discussion (page unavailable) says apoA-V accrues on cytosolic lipid droplets, supporting the apoA-V part of the sentence. The paper does not address apoC-III localization, so it cannot support that part of the claim. (LLM, conf=0.86)
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Research works printed but not linked: 20
- [1]Johansen CT Wang J Lanktree MB Cao H McIntyre AD Ban MR Martins RA Kennedy BA Hassell RG Visser ME Schwartz SM Voight BF Elosua R Salomaa V O'Donnell CJ Dallinga-Thie GM Anand SS Yusuf S Huff MW Kathiresan S Hegele RA Excess of rare variants in genes identified by genome-wide association study of hypertriglyceridemia Nat. Genet 2010 42 684 687 20657596 PMC3017369
- [2]Johansen CT Kathiresan S Hegele RA Genetic determinants of plasma triglycerides J. Lipid Res 2011 52 189 206 21041806 PMC3023540
- [3]Pennacchio LA Olivier M Hubacek JA Cohen JC Cox DR Fruchart JC Krauss RM Rubin EM An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing Science 2001 294 169 173 11588264
- [4]Sharma V Ryan RO Forte TM Apolipoprotein A-V dependent modulation of plasma triacylglycerol: A puzzlement Biochim. Biophys. Acta 2012 1821 795 799 22209939 PMC3319174
- [5]Van der Vliet HN Sammels MG Leegwater AC Levels JH Reitsma PH Boers W Chamuleau RA Apolipoprotein A-V: a novel apolipoprotein associated with an early phase of liver regeneration J. Biol. Chem 2001 276 44512 44520 11577099
- [6]Weinberg RB Cook VR Beckstead JA Martin DD Gallagher JW Shelness GS Ryan RO Structure and interfacial properties of human apolipoprotein A-V J. Biol. Chem 2003 278 34438 34444 12810715
- [7]Schaap FG Rensen PCN Voshol PJ Vrins C Van der Vliet HN Chamuleau RA Havekes LM Groen AK Van Dijk KW ApoAV reduces plasma triglycerides by Inhibiting very low density lipoprotein-triglyceride (VLDL-TG) production and stimulating lipoprotein lipase-mediated VLDL-TG hydrolysis J. Biol. Chem 2004 279 27941 27947 15090553
- [8]Van der Vliet HN Schaap FG Levels JHM Ottenhoff R Looije N Wesseling JG Groen AK Chamuleau RA Adenoviral overexpression of apolipoprotein A-V reduces serum levels of triglycerides and cholesterol in mice Biochem. Biophys. Res. Commun 2002 295 1156 1159 12135615
- [9]Shu X Chan J Ryan RO Forte TM ApoA-V association with intracellular lipid droplets J. Lipid Res 2007 48 1445 1450 17460328
- [10]Shu X Ryan RO Forte TM Intracellular lipid droplet targeting by apolipoprotein A-V requires the carboxyl-terminal segment J. Lipid Res 2008 49 1670 1676 18450648 PMC2444012
- [11]Shu X Nelbach L Ryan RO Forte TM Apolipoprotein A-V associates with intrahepatic lipid droplets and influences triglyceride accumulation Biochim. Biophys. Acta 2010 1801 605 608 20153840 PMC2839894
- [12]Walther TC Farese RV Jr Lipid droplets and cellular lipid metabolism Annu. Rev. Biochem 2012 81 687 714 22524315 PMC3767414
- [13]Pamir N McMillen TS Li YI Lai CM Wong H LeBoeuf RC Overexpression of apolipoprotein A5 in mice is not protective against body weight gain and aberrant glucose homeostasis Metabolism 2009 58 560 567 19303979 PMC2689095
- [14]Blade AM Fabritius MA Hou L Weinberg RB Shelness GS Biogenesis of apolipoprotein A-V and its impact on VLDL triglyceride secretion J. Lipid Res 2011 52 237 244 21115968 PMC3023543
- [15]Greenspan P Mayer EP Fowler SD Nile red: a selective fluorescent stain for intracellular lipid droplets J. Cell Biol 1985 100 965 973 3972906 PMC2113505
- [16]Martinez-Jimenez CP mez-Lechon MJG Castell JV Jover R Underexpressed coactivators PGC1 and SRC1 impair hepatocyte nuclear factor 4 function and promote dedifferentiation in human hepatoma cells J. Biol. Chem 2006 281 29840 29849 16891307
- [17]O’Brien PJ Alborn WE Sloan JH Ulmer M Boodhoo A Knierman MD Schultze AE Konrad RJ The novel apolipoprotein A5 is present in human serum, is associated with VLDL, HDL, and chylomicrons, and circulates at very low concentrations compared with other apolipoproteins Clin. Chem 2005 51 351 359 15528295
- [18]Martin S Parton RG Lipid droplets: a unified view of a dynamic organelle Nat. Rev. Mol. Cell Biol 2006 7 373 378 16550215
- [19]Ploegh HL A lipid-based model for the creation of an escape hatch from the endoplasmic reticulum Nature 2007 448 435 438 17653186
- [20]Sundaram M Yao Z Intrahepatic role of exchangeable apolipoproteins in lipoprotein assembly and secretion Arterioscler. Thromb. Vasc. Biol 2012 32 1073 1078 22517365
Cited by
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APOE traffics to astrocyte lipid droplets and modulates triglyceride saturation and droplet size
Windham, I. A., Powers, A. E., Ragusa, J. V., Wallace, E. D., Zanellati, M. C., Williams, V. H., Wagner, C. H., White, K. K., & Cohen, S. (2024) · Journal of Cell Biology · doi:10.1083/jcb.202305003 · Cited by 96 · see citing works
- Authors
- Gao, X., Forte, T. M., & Ryan, R. O.
- Corresponding author
- Ryan, Robert O.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2012-10
- Volume
- 427
- Issue
- 2
- Pages
- 361-365
- ISSN
- 0006-291X (ISSN-L)
- PMC
- PMC3492953
- OpenAlex
- W1981887910
- Cited by
- 16
- References
- 0
- Citations by year
- 2024: 1 · 2023: 1 · 2022: 2 · 2021: 3 · 2019: 1 · 2017: 1 · 2015: 2 · 2014: 3 · 2013: 1 · 2012: 1
- FWCI
- 0.96
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Heart, Lung, and Blood Institute (HL 64159, R37HL064159, R01HL064159)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Health Sciences › Medicine › Surgery › Cholesterol and Lipid Metabolism
- Keywords
- apolipoprotein A-V, lipid droplets, hepatocytes, hepatic lipid metabolism, stable transfection, clonal selection
- MeSH terms
- Apolipoprotein A-V, Apolipoproteins A, Cytosol, Humans, Transfection, Triglycerides, Hepatocytes, Cell Line, Tumor
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