SPION primes THP1 derived M2 macrophages towards M1-like macrophages
Abstract
Potentially, cellular iron regulates functional plasticity in macrophages yet; interaction of functionally polarized macrophages with iron-oxide nanoparticles has never been studied. We found that monocyte differentiation alters cellular ferritin and cathepsin L levels and induces functional polarization in macrophages. Iron in super paramagnetic iron-oxide nanoparticle (SPION) induces a phenotypic shift in THP1 derived M2 macrophages towards a high CD86+ and high TNF α+ macrophage subtype. This phenotypic shift was accompanied by up-regulated intracellular levels of ferritin and cathepsin L in M2 macrophages, which is a characteristic hallmark of M1 macrophages. Atherogenic oxysterols reduce phagocytic activity in macrophage subtypes, and thus these cells may escape detection by iron-oxide nanoparticles (INPs) in-vivo.
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SPION treatment of human macrophages pre-polarized to M2-like state causes a shift toward M1 phenotype with increased CD86, TNFα, and intracellular ferritin.
“As in the above study, they observed a shift in phenotype towards M1, which comes along with an increased expression of CD86, TNFα and intracellular ferritin [213].”
From Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications (2019) · cites this paper as [213]
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References 32 · Cited by 138
- Authors
- Laskar, A., Eilertsen, J., Li, W., & Yuan, X.-M.
- Affiliations
- Linköping University (SE)
- Linköping University Hospital (SE)
- Corresponding author
- Laskar, Amit
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 2013-11
- Volume
- 441
- Issue
- 4
- Pages
- 737-742
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W2013899855
- Cited by
- 138
- References
- 32
- Citations by year
- 2026: 8 · 2025: 16 · 2024: 15 · 2023: 11 · 2022: 17 · 2021: 12 · 2020: 14 · 2019: 18 · 2018: 7 · 2017: 9 · 2016: 6 · 2015: 3 · 2014: 2
- FWCI
- 2.03
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Swedish Heart Lung Foundation
- Linköping University Hospital Research foundation
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Immune cells in cancer
- Physical Sciences › Engineering › Biomedical Engineering › Characterization and Applications of Magnetic Nanoparticles
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Keywords
- superparamagnetic iron oxide nanoparticles, M2 macrophages, macrophage polarization, M1 macrophage phenotype, THP-1 cells, ferritin, cathepsin L, CD86, TNF-α, oxysterols, phagocytosis, monocyte differentiation
- MeSH terms
- Cell Differentiation, Cells, Cultured, Dextrans, Ferric Compounds, Ferritins, Humans, Immunoglobulin G, Inflammation, Macrophages, Phagocytosis, Sterols, Tetradecanoylphorbol Acetate, Tumor Necrosis Factor-alpha, Up-Regulation, Cell Polarity, B7-2 Antigen, Cathepsin L, Magnetite Nanoparticles, Plaque, Atherosclerotic
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