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Exosomal microRNA-1 and MYO15A as a target for therapy and diagnosis in renal cell carcinoma

Yoshino, Hirofumi, Tatarano, Shuichi, Tamai, Motoki, Tsuruda, Masafumi, Iizasa, Sayaka, Arima, Junya, Kawakami, Issei, Fukumoto, Wataru, Kawahara, Ichiro, Li, Gang, Sakaguchi, Takashi, Inoguchi, Satoru, Yamada, Yasutoshi, Enokida, Hideki·2022·Biochemical and Biophysical Research Communications
DOI 10.1016/j.bbrc.2022.09.056
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CC-BY-NC · From the PDF
Fig. 1 Exosome isolation using the spin column method. (A) Schema of the method for isolation of exosomes from cell culture medium or human serum. (B, C) Identi fi cation of exosomes from cell culture medium or human serum by immunoblotting (left) and NTA (right).
Fig. 1 Exosome isolation using the spin column method. (A) Schema of the method for isolation of exosomes from cell culture medium or human serum. (B, C) Identi fi cation of exosomes from cell culture medium or human serum by immunoblotting (left) and NTA (right).CC-BY-NC · From the PDF
Fig. 2 Exosomal miR-1 reduced viabilities of treated cells. (A) Schema of the method used to isolate exosomes from donor cells transfected by miR-1 , followed by the transfer of exosomal miR-1 to recipient cells. (B) Fluorescence images of 786-o and A498 cells treated with PKH26-stained exosomes. (C) miR-1 expression was investigated by RT-qPCR in recipient RCC cells following the addition of miR-1 -transfected cell-derived exosomes (upper), and in RCC cells in which miR-1 was induced by a normal transfection method (lower). (D) Cell proliferation was evaluated using XTT assays (n ¼ 6). * , P < 0.05. (E) Cell migration activity was measured using wound healing assays (n ¼ 9). * , P < 0.001. (F) Cell invasion activity was measured using Matrigel invasion assays (n ¼ 8). * , P < 0.01.
Fig. 2 Exosomal miR-1 reduced viabilities of treated cells. (A) Schema of the method used to isolate exosomes from donor cells transfected by miR-1 , followed by the transfer of exosomal miR-1 to recipient cells. (B) Fluorescence images of 786-o and A498 cells treated with PKH26-stained exosomes. (C) miR-1 expression was investigated by RT-qPCR in recipient RCC cells following the addition of miR-1 -transfected cell-derived exosomes (upper), and in RCC cells in which miR-1 was induced by a normal transfection method (lower). (D) Cell proliferation was evaluated using XTT assays (n ¼ 6). * , P < 0.05. (E) Cell migration activity was measured using wound healing assays (n ¼ 9). * , P < 0.001. (F) Cell invasion activity was measured using Matrigel invasion assays (n ¼ 8). * , P < 0.01.CC-BY-NC · From the PDF
Fig. 3 Analysis of miR-1 and MYO15A in TCGA kidney clear cell carcinoma (KIRC) datasets, and of MYO15A in serum exosomal RNA. (A, C upper) The expression level of miR-1 (A) and MYO15A (C) in ccRCC tissues and in normal kidney tissues according to TCGA data. (A, C lower) Overall survival of patients with high or low expression of miR-1 (A) and MYO15A (C) in ccRCC by Kaplan-Meier survival curves. P-values were calculated using the log-rank test. (B) Exosomal MYO15A mRNA expression in clinical RCC samples compared with that in clinical normal samples (upper), with & pT1b and S pT2a (lower).
Fig. 3 Analysis of miR-1 and MYO15A in TCGA kidney clear cell carcinoma (KIRC) datasets, and of MYO15A in serum exosomal RNA. (A, C upper) The expression level of miR-1 (A) and MYO15A (C) in ccRCC tissues and in normal kidney tissues according to TCGA data. (A, C lower) Overall survival of patients with high or low expression of miR-1 (A) and MYO15A (C) in ccRCC by Kaplan-Meier survival curves. P-values were calculated using the log-rank test. (B) Exosomal MYO15A mRNA expression in clinical RCC samples compared with that in clinical normal samples (upper), with & pT1b and S pT2a (lower).CC-BY-NC · From the PDF

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Research works printed but not linked: 20

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Metadata

Authors
Yoshino, H., Tatarano, S., Tamai, M., Tsuruda, M., Iizasa, S., Arima, J., Kawakami, I., Fukumoto, W., Kawahara, I., Li, G., Sakaguchi, T., Inoguchi, S., Yamada, Y., & Enokida, H.
Venue
Biochemical and Biophysical Research Communications
Publisher
Elsevier BV
Published
2022-11
Volume
630
Pages
71-76
ISSN
0006-291X
DOI
10.1016/j.bbrc.2022.09.056
Cited by
24
References
0
Language
en
Licence
Funders
  • Kagoshima University
  • Japan Society for the Promotion of Science (21K09404, 21K09430, 22K09452, 22K09507, 22K16792, 22K16820)
  • Takeda Science Foundation

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Yoshino, H., Tatarano, S., Tamai, M., Tsuruda, M., Iizasa, S., Arima, J., Kawakami, I., Fukumoto, W., Kawahara, I., Li, G., Sakaguchi, T., Inoguchi, S., Yamada, Y., & Enokida, H. (2022). Exosomal microRNA-1 and MYO15A as a target for therapy and diagnosis in renal cell carcinoma. Biochemical and Biophysical Research Communications. https://doi.org/10.1016/j.bbrc.2022.09.056