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Cited by 91Nanoparticle-Based Drug DeliveryOral microbiology and periodontitis researchAdvanced Drug Delivery Systems

pH and lipase-responsive nanocarrier-mediated dual drug delivery system to treat periodontitis in diabetic rats

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Wang, Lu, Li, Yuzhou, Ren, Mingxing, Wang, Xu, Li, Lingjie, Liu, Fengyi, Lan, Yiqing, Yang, Sheng +1 more·2022·Bioactive Materials
DOI 10.1016/j.bioactmat.2022.02.008PMC PMC8961308
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Abstract

Precise and controlled drug delivery to treat periodontitis in patients with diabetes remains a significant clinical challenge. Nanoparticle-based drug delivery systems offer a potential therapeutic strategy; however, the low loading efficiency, non-responsiveness, and single effect of conventional nanoparticles hinder their clinical application. In this study, we designed a novel self-assembled, dual responsive, and dual drug-loading nanocarrier system, which comprised two parts: the hydrophobic lipid core formed by 1, 2-Distearoyl-sn-glycero-3-phosphoethanolamine-Poly (ethylene glycol) (DSPE-PEG) loaded with alpha-lipoic acid (ALA); and a hydrophilic shell comprising a poly (amidoamine) dendrimer (PAMAM) that electrostatically adsorbed minocycline hydrochloride (Mino). This unique design allows the controlled release of antioxidant/ALA under lipase stimulation from periodontal pathogens and antimicrobial/Mino under the low pH of the inflammatory microenvironment. In vivo and in vitro studies confirmed that this dual nanocarrier could inhibit the formation of subgingival microbial colonies while promoting osteogenic differentiation of cells under diabetic pathological conditions, and ameliorated periodontal bone resorption. This effective and versatile drug-delivery strategy has good potential applications to inhibit diabetes-associated periodontal bone loss.

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  • These intelligent PDA-based delivery systems attenuate alveolar bone resorption.

    “Specifically, these systems have been shown to suppress periodontal inflammation, attenuate alveolar bone resorption, and promote periodontal tissue regeneration.68,73,78”

    From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [73]

    The Introduction (Scheme 1) says the nanocarrier ameliorated periodontal bone resorption in diabetic rats, and the Results report reduced bone-loss measurements with DPPLM treatment. But the paper identifies its carrier as DSPE-PEG-PAMAM, not a PDA-based system, so it does not support that description. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • Extensive in vitro release studies and in vivo evaluations, including rat models of periodontitis, have consistently demonstrated the effectiveness of these intelligent PDA-based delivery platforms.

    “Extensive in vitro release studies and in vivo evaluations, including rat models of periodontitis, have consistently demonstrated the effectiveness of these intelligent PDA-based delivery platforms.”

    From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [73]

    The Introduction and Conclusion report in vitro drug-release testing and effective treatment in a diabetic-rat periodontitis model. But the platform studied is DSPE-PEG-PAMAM, not PDA-based, and this single study does not establish consistent effectiveness across multiple platforms. Page numbers are unavailable in our copy. (LLM, conf=0.88)

    Experimental — accuracy not yet measured

  • These intelligent PDA-based delivery systems promote periodontal tissue regeneration.

    “Specifically, these systems have been shown to suppress periodontal inflammation, attenuate alveolar bone resorption, and promote periodontal tissue regeneration.68,73,78”

    From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [73]

    The Results (page not provided) report denser, better-arranged periodontal ligaments in treated diabetic rats and enhanced osteogenic differentiation in vitro, supporting a tissue-repair effect. But the Introduction and Conclusion identify this carrier as DSPE-PEG-PAMAM, not PDA-based; the in vivo conclusion is chiefly that it inhibited periodontal bone loss, rather than demonstrated full tissue regeneration. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • These intelligent PDA-based delivery systems suppress periodontal inflammation.

    “Specifically, these systems have been shown to suppress periodontal inflammation, attenuate alveolar bone resorption, and promote periodontal tissue regeneration.68,73,78”

    From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [73]

    The Results section reports that DPPLM nanoparticles inhibited inflammatory processes in vitro by reducing ROS and iNOS. But the Introduction identifies the carrier as DSPE-PEG-PAMAM, not PDA-based; the paper therefore supports the anti-inflammatory effect, not the claimed carrier identity. (LLM, conf=0.87)

    Experimental — accuracy not yet measured

  • In vitro antibacterial assays, simplified biofilm models, release studies, and selected animal models show that PDA-based systems can achieve biofilm inhibition, ROS regulation, localized drug release, photothermal/photodynamic antibacterial effects, and inflammation control.

    “In vitro antibacterial assays, simplified biofilm models, release studies, and selected animal models show biofilm inhibition, ROS regulation, localized drug release, photothermal/photodynamic antibacterial effects, and inflammation control.”

    From Polydopamine-Based Biomaterials in Oral Medicine: Applications, Mechanisms, and Translational Challenges (2026) · cites this paper as [73]

    Split into 2 atoms — 1 confirmed, 1 partially supported, 0 disputed, 0 misattributed, 0 unverifiable. Expand the claim to see per-atom verdicts.

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Authors
Wang, L., Li, Y., Ren, M., Wang, X., Li, L., Liu, F., Lan, Y., Yang, S., & Song, J.
Affiliations
Chongqing Medical University (CN)
Corresponding author
  • Yang, Sheng
  • Song, Jinlin
ORCID
Venue
Bioactive Materials
Publisher
Elsevier BV
Published
2022-12
Volume
18
Pages
254-266
ISSN
  • 2097-1192 (ISSN-L)
  • 2452-199X
DOI
10.1016/j.bioactmat.2022.02.008
PMC
PMC8961308
OpenAlex
W4213305258
Cited by
91
References
61
Citations by year
2026: 8 · 2025: 29 · 2024: 31 · 2023: 17 · 2022: 6
FWCI
5.19
Type
journal article
Language
en
Open access
Licence
Locations
  • Bioactive Materials (publishedVersion, cc-by-nc-nd)
  • PubMed Central (publishedVersion, cc-by-nc-nd)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
  • Chongqing Municipal Education Commission (KJQN201900441)
  • Chongqing Science and Technology Commission (cstc2021jcyj-jqX0028, cstc2019jcyj-bshX0005, cstc2019jcyj-msxmX0366)
  • Chongqing Medical University (KQFBD002)
  • National Natural Science Foundation of China (82001103, 81771082, 82171010, 31971282)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
Keywords
periodontitis, diabetes mellitus, dual drug delivery, pH-responsive drug release, nanocarriers, alpha-lipoic acid, minocycline hydrochloride, PAMAM dendrimers, periodontal bone loss, osteogenic differentiation, subgingival bacteria, periodontal pathogens

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