Factors effecting expression of vaccines in microalgae
Abstract
PhycoBiologics is developing an oral vaccine delivery system using vaccines expressed in the chloroplast of microalgae. Despite many advances in plastid transformation technology, levels of expression remain inconsistent. We have concluded that the main factors affecting the level of recombinant protein expression in the chloroplast of Chlamydomonas are: codon optimization, protease activity, protein toxicity and transformation-associated genotypic modification.
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Chlamydomonas reinhardtii has chloroplast features well-suited for developing a delivery system for oral vaccines, which will be helpful for developing a microalgal vaccine strategy for immunizing aquatic animals.
“Chlamydomonas reinhardtii (C. reinhardtii) is known to have chloroplast features well-suited for developing a delivery system for oral vaccines (Surzycki et al., 2009), which will be helpful for developing a microalgal vaccine strategy for immunizing aquatic animals.”
From Microalgae Metabolites: A Rich Source for Food and Medicine (2019) · cites this paper as Surzycki et al., 2009
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References 19 · Cited by 185
- Authors
- Surzycki, R., Greenham, K., Kitayama, K., Dibal, F., Wagner, R., Rochaix, J.-D., Ajam, T., & Surzycki, S.
- Affiliations
- University of Geneva (CH)
- Indiana University Bloomington (US)
- Corresponding author
- Wagner, Richard
- Venue
- Biologicals
- Publisher
- Elsevier BV
- Published
- 2009-06
- Volume
- 37
- Issue
- 3
- Pages
- 133-138
- ISSN
- 1045-1056 (ISSN-L)
- OpenAlex
- W2011969555
- Cited by
- 185
- References
- 19
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 6 · 2023: 10 · 2022: 13 · 2021: 8 · 2020: 10 · 2019: 15 · 2018: 8 · 2017: 14 · 2016: 13 · 2015: 14 · 2014: 21 · 2013: 13 · 2012: 12
- FWCI
- 8.71
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biologicals (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Transgenic Plants and Applications
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- recombinant protein expression, chloroplast transformation, microalgae, Chlamydomonas, oral vaccine delivery, codon optimization, proteolytic activity, protein toxicity, plastid transformation
- MeSH terms
- Animals, Chlamydomonas, Codon, Genotype, Vaccines, Synthetic, Blotting, Western, Animals, Genetically Modified
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