Removing residual DNA from Vero-cell culture-derived human rabies vaccine by using nuclease
Abstract
The clearance of host cell DNA is a critical indicator for Vero-cell culture-derived rabies vaccine. In this study, we evaluated the clearance of DNA in Vero-cell culture-derived rabies vaccine by purification process utilizing ultrafiltration, nuclease digestion, and gel filtration chromatography. The results showed that the bioprocess of using nuclease decreased residual DNA. Dot-blot hybridization analysis showed that the residual host cell DNA was 5.0 IU/ml. Immunogenicity test and protection experiments indicated mice were greatly induced generation of neutralizing antibodies and invoked protective effects immunized with intraperitoneal injections of the rabies vaccine. These results demonstrated that the residual DNA was removed from virus particles and nuclease was removed by gel filtration chromatography. The date indicated that technology was an efficient method to produce rabies vaccine for human use by using nuclease.
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The nuclease process increases production costs and carries safety uncertainties.
“In addition, this process increases production costs [29,30] and carries safety uncertainties.”
From Evaluation of the Efficacy of the Vaccine Production Process in Removing Residual Host Cell DNA from the Vero Cell Rabies Vaccine (2024) · cites this paper as [29]
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Li, J., Pan, R., Yue, F., Gao, T., Wu, X., Shi, L., Wang, Y., Zhao, D., Lan, Z., Chen, H., et al. (2024) · Vaccines · doi:10.3390/vaccines12121379 · Cited by 8 · see citing works
- Authors
- Li, S.-M., Bai, F.-L., Xu, W.-J., Yang, Y.-B., An, Y., Li, T.-H., Yu, Y.-H., Li, D.-S., & Wang, W.-F.
- Affiliations
- Northeast Agricultural University (CN)
- University of Chinese Academy of Sciences (CN)
- ORCID
- An, Ying
- Venue
- Biologicals
- Publisher
- Elsevier BV
- Published
- 2014-09
- Volume
- 42
- Issue
- 5
- Pages
- 271-276
- ISSN
- 1045-1056 (ISSN-L)
- OpenAlex
- W2014589355
- Cited by
- 16
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 5 · 2023: 2 · 2021: 1 · 2020: 2 · 2019: 1 · 2016: 3
- FWCI
- 0.45
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biologicals (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Heilongjiang (QC2012C099)
- Northeast Agriculture University (2012RCB43)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › Rabies epidemiology and control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein purification and stability
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Keywords
- rabies vaccine, Vero cell culture, nuclease digestion, size exclusion chromatography, ultrafiltration, vaccine purification, freeze-drying stability, neutralizing antibodies, immunogenicity
- MeSH terms
- Animals, Antibodies, Viral, Chlorocebus aethiops, Chromatography, Gel, DNA, Drug Contamination, Drug Stability, Endodeoxyribonucleases, Endoribonucleases, Freeze Drying, Humans, Rabies, Rabies Vaccines, Rabies virus, Vero Cells, Mice, Antibodies, Neutralizing
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