The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
The microwell control of embryoid body size in order to regulate cardiac differentiation of human embryonic stem cells
Open accessAbstract
The differentiation of human embryonic stem cells (hESCs) into cardiomyocytes (CMs) using embryoid bodies (EBs) is relatively inefficient and highly variable. Formation of EBs using standard enzymatic disaggregation techniques results in a wide range of sizes and geometries of EBs. Use of a 3-D cuboidal microwell system to culture hESCs in colonies of defined dimensions, 100 to 500 μm in lateral dimensions and 120 μm in depth, enabled formation of more uniform sized EBs. The 300 μm microwells produced highest percentage of contracting EBs, but flow cytometry for myosin light chain 2A (MLC2a) expressing cells revealed a similar percentage (~3%) of cardiomyocytes formed in EBs from 100 μm and 300 μm microwells. These data, and immunolabeling with anti-MF20 and MLC2a, suggest that the smaller EBs are less likely to form contracting EBs, but those contracting EBs are relatively enriched in cardiomyocytes compared to larger EB sizes where CMs make up a proportionately smaller fraction of the total cells. We conclude that microwell-engineered EB size regulates cardiogenesis and can be used for more efficient and reproducible formation of hESC-CMs needed for research and therapeutic applications.
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Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310
Self-citationHuman cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population
Yang, L., Soonpaa, M. H., Adler, E. D., Roepke, T. K., Kattman, S. J., Kennedy, M., Henckaerts, E., Bonham, K., Abbott, G. W., Linden, R. M., et al. (2008) · Nature · doi:10.1038/nature06894 · Cited by 1,389
He, J.-Q., Ma, Y., Lee, Y., Thomson, J. A., & Kamp, T. J. (2003) · Circulation Research · doi:10.1161/01.res.0000080317.92718.99 · Cited by 800
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Cited by
Lian, X., Zhang, J., Azarin, S. M., Zhu, K., Hazeltine, L. B., Bao, X., Hsiao, C., Kamp, T. J., & Palecek, S. P. (2013) · Nature Protocols · doi:10.1038/nprot.2012.150 · Cited by 1,748
Self-citationBioprinting for stem cell research
Tasoglu, S., & Demirci, U. (2013) · Trends in Biotechnology · doi:10.1016/j.tibtech.2012.10.005 · Cited by 422
In vitro cardiac tissue models: Current status and future prospects
Mathur, A., Ma, Z., Loskill, P., Jeeawoody, S., & Healy, K. E. (2016) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2015.09.011 · Cited by 187
Guidance of stem cell fate on 2D patterned surfaces
Kolind, K., Leong, K. W., Besenbacher, F., & Foss, M. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.05.070 · Cited by 163
Hoang, P., Wang, J., Conklin, B. R., Healy, K. E., & Ma, Z. (2018) · Nature Protocols · doi:10.1038/nprot.2018.006 · Cited by 156
Metadata
- Authors
- Mohr, J. C., Zhang, J., Azarin, S. M., Soerens, A. G., de Pablo, J. J., Thomson, J. A., Lyons, G. E., Palecek, S. P., & Kamp, T. J.
- Venue
- Biomaterials
- Publisher
- Elsevier BV
- Published
- 2010-03
- Volume
- 31
- Issue
- 7
- Pages
- 1885-1893
- ISSN
- 0142-9612 (ISSN-L)
- OpenAlex
- W1997910229
- Cited by
- 218
- References
- 3
- FWCI
- 6.72
- Type
- journal article
- Language
- en
- Open access
- green
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- Mohr, J. C., Zhang, J., Azarin, S. M., Soerens, A. G., de Pablo, J. J., Thomson, J. A., Lyons, G. E., Palecek, S. P., & Kamp, T. J. (2010). The microwell control of embryoid body size in order to regulate cardiac differentiation of human embryonic stem cells. Biomaterials. https://doi.org/10.1016/j.biomaterials.2009.11.033
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
