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Cited by 36Hair Growth and DisordersTissue Engineering and Regenerative Medicinedental development and anomalies

Inducing hair follicle neogenesis with secreted proteins enriched in embryonic skin

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Fan, Sabrina Mai-Yi, Tsai, Chia-Feng, Yen, Chien-Mei, Lin, Miao-Hsia, Wang, Wei-Hung, Chan, Chih-Chieh, Chen, Chih-Lung, Phua, Kyle K.L. +5 more·2018·Biomaterials
DOI 10.1016/j.biomaterials.2018.03.003PMC PMC6050066
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Abstract

Organ development is a sophisticated process of self-organization. However, despite growing understanding of the developmental mechanisms, little is known about how to reactivate them postnatally for regeneration. We found that treatment of adult non-hair fibroblasts with cell-free extract from embryonic skin conferred upon them the competency to regenerate hair follicles. Proteomics analysis identified three secreted proteins enriched in the embryonic skin, apolipoprotein-A1, galectin-1 and lumican that together were essential and sufficient to induce new hair follicles. These 3 proteins show a stage-specific co-enrichment in the perifolliculogenetic embryonic dermis. Mechanistically, exposure to embryonic skin extract or to the combination of the 3 proteins altered the gene expression to an inductive hair follicle dermal papilla fibroblast-like profile and activated Igf and Wnt signaling, which are crucial for the regeneration process. Therefore, a cocktail of organ-specific extracellular proteins from the embryonic environment can render adult cells competent to re-engage in developmental interactions for organ neogenesis. Identification of factors that recreate the extracellular context of respective developing tissues can become an important strategy to promote regeneration in adult organs.

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  • ECM extracts from fetal skin promote hair regeneration after transplantation with epithelial and mesenchymal cells.

    “ECM extracts from the fetal skin promote hair regeneration after transplantation with epithelial and mesenchymal cells (43).”

    From Reprogramming of three-dimensional microenvironments for in vitro hair follicle induction (2022) · cites this paper as [43]

    Results §3.4 reports that embryonic skin extract gave adult fibroblasts hair-forming competence before they were combined with keratinocytes in patch assays. The methods describe injection of these epithelial and mesenchymal cells into mice. The source calls the material a cell-free embryonic skin extract, however, not a specifically isolated ECM extract. (LLM, conf=0.83)

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References 87 · Cited by 36

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Authors
Fan, S. M.-Y., Tsai, C.-F., Yen, C.-M., Lin, M.-H., Wang, W.-H., Chan, C.-C., Chen, C.-L., Phua, K. K. L., Pan, S.-H., Plikus, M. V., Yu, S.-L., Chen, Y.-J., & Lin, S.-J.
Affiliations
  • National Taiwan University (TW)
  • Institute of Chemistry, Academia Sinica (TW)
  • National University of Singapore (SG)
  • University of California, Irvine (US)
Corresponding author
  • Chen, Yu-Ju
  • Lin, Sung-Jan
ORCID
Venue
Biomaterials
Publisher
Elsevier BV
Published
2018-06
Volume
167
Pages
121-131
ISSN
0142-9612 (ISSN-L)
DOI
10.1016/j.biomaterials.2018.03.003
PMC
PMC6050066
OpenAlex
W2793761216
Cited by
36
References
87
Citations by year
2026: 2 · 2025: 6 · 2023: 3 · 2022: 6 · 2021: 5 · 2020: 7 · 2019: 7
FWCI
6.34
Type
journal article
Language
en
Open access
Licence
Locations
  • Biomaterials (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
Funders
  • Taiwan Ministry of Science and Technology (103-2325-B-002-008, 103–2628-B002-004-MY3, 104-2627-M-002-012)
  • National Taiwan University Hospital (NTU-CDP-103R7879, NTU-CDP-1104R7879)
  • National Taiwan University (104R7602D4)
  • NIH (R01-AR067273)
Topics
  • Health Sciences › Medicine › Urology › Hair Growth and Disorders
  • Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › dental development and anomalies
Keywords
hair follicle neogenesis, hair follicle regeneration, apolipoprotein A-I, galectin-1, lumican, dermal papilla, fibroblast reprogramming, IGF signaling, Wnt signaling, extracellular matrix proteins, organ regeneration
MeSH terms
Lumican, Animals, Cells, Cultured, Female, Fibroblasts, Mice, Inbred BALB C, Mice, Inbred C57BL, Regeneration, Skin, Apolipoprotein A-I, Rats, Wistar, Hair Follicle, Galectin 1

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