Cited by 33

The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.

Immersion Fixation and Staining of Multicubic Millimeter Volumes for Electron Microscopy–Based Connectomics of Human Brain Biopsies

Karlupia, Neha, Schalek, Richard L., Wu, Yuelong, Meirovitch, Yaron, Wei, Donglai, Charney, Alexander W., Kopell, Brian H., Lichtman, Jeff W.·2023·Biological Psychiatry

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1016/j.biopsych.2023.01.025

Cites

4 of 4 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 4 references

Research works printed but not linked: 60

  1. Kasthuri, 2015, Saturated reconstruction of a volume of neocortex, Cell, 162, 648, 10.1016/j.cell.2015.06.054 · DOI · Paper page
  2. Morgan, 2016, The fuzzy logic of network connectivity in mouse visual thalamus, Cell, 165, 192, 10.1016/j.cell.2016.02.033 · DOI
  3. Hua, 2015, Large-volume en-bloc staining for electron microscopy-based connectomics, Nat Commun, 6, 7923, 10.1038/ncomms8923 · DOI
  4. Mikula, 2015, High-resolution whole-brain staining for electron microscopic circuit reconstruction, Nat Methods, 12, 541, 10.1038/nmeth.3361 · DOI
  5. Yin, 2020, A petascale automated imaging pipeline for mapping neuronal circuits with high-throughput transmission electron microscopy, Nat Commun, 11, 4949, 10.1038/s41467-020-18659-3 · DOI
  6. Witvliet, 2021, Connectomes across development reveal principles of brain maturation, Nature, 596, 257, 10.1038/s41586-021-03778-8 · DOI · Paper page
  7. Tapia, 2012, High-contrast en bloc staining of neuronal tissue for field emission scanning electron microscopy, Nat Protoc, 7, 193, 10.1038/nprot.2011.439 · DOI · Paper page
  8. Morgan, 2013, Why not connectomics?, Nat Methods, 10, 494, 10.1038/nmeth.2480 · DOI
  9. Lichtman, 2014, The big data challenges of connectomics, Nat Neurosci, 17, 1448, 10.1038/nn.3837 · DOI
  10. Abbott, 2017, Researchers add live human cells to brain database [published online May 30], Nature, 10.1038/nature.2017.22889 · DOI
  11. Shapson-Coe, 2021, A connectomic study of a petascale fragment of human cerebral cortex, bioRxiv
  12. Loomba, 2022, Connectomic comparison of mouse and human cortex, Science, 377, 10.1126/science.abo0924 · DOI · Paper page
  13. Cascella, 2021, Deep brain stimulation of the substantia nigra pars reticulata for treatment-resistant schizophrenia: A case report [published correction appears in Biol Psychiatry 2021;90:729], Biol Psychiatry, 90, e57, 10.1016/j.biopsych.2021.03.007 · DOI
  14. Singh, 1982, Scanning and transmission electron microscopy of the ependyma of the fourth ventricle in the monkey brain, Acta Anat (Basel), 112, 365, 10.1159/000145529 · DOI
  15. Eid, 2017, Preparation of non-human primate brain tissue for pre-embedding immunohistochemistry and electron microscopy, J Vis Exp, 122
  16. Song, 2023, High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics, Nat Methods, 20, 836, 10.1038/s41592-023-01866-3 · DOI
  17. Beach, 1987, Perfusion-fixation of the human brain for immunohistochemistry: Comparison with immersion-fixation, J Neurosci Methods, 19, 183, 10.1016/S0165-0270(87)80001-8 · DOI
  18. Castejon, 2009, The extracellular space in the edematous human cerebral cortex: An electron microscopic study using cortical biopsies, Ultrastruct Pathol, 33, 102, 10.1080/01913120902787076 · DOI
  19. Witcher, 2010, Three-dimensional relationships between perisynaptic astroglia and human hippocampal synapses, Glia, 58, 572, 10.1002/glia.20946 · DOI
  20. Blazquez-Llorca, 2013, FIB/SEM technology and Alzheimer’s disease: Three-dimensional analysis of human cortical synapses, J Alzheimers Dis, 34, 995, 10.3233/JAD-122038 · DOI
  21. Domínguez-Álvaro, 2019, 3D electron microscopy study of synaptic organization of the normal human transentorhinal cortex and its possible alterations in Alzheimer’s disease, eNeuro, 6, 10.1523/ENEURO.0140-19.2019 · DOI
  22. Yakoubi, 2019, Ultrastructural heterogeneity of layer 4 excitatory synaptic boutons in the adult human temporal lobe neocortex, eLife, 8, 10.7554/eLife.48373 · DOI
  23. Zhang, 2020, Circuit reorganization shapes the developing human foveal midget connectome toward single-cone resolution, Neuron, 108, 905, 10.1016/j.neuron.2020.09.014 · DOI
  24. Hayworth, 2014, Imaging ATUM ultrathin section libraries with WaferMapper: A multi-scale approach to EM reconstruction of neural circuits, Front Neural Circuits, 8, 68, 10.3389/fncir.2014.00068 · DOI · Paper page
  25. Eberle, 2015, High-resolution, high-throughput imaging with a multibeam scanning electron microscope, J Microsc, 259, 114, 10.1111/jmi.12224 · DOI
  26. Saalfeld, 2012, Elastic volume reconstruction from series of ultra-thin microscopy sections, Nat Methods, 9, 717, 10.1038/nmeth.2072 · DOI · Paper page
  27. Meirovitch, 2016, A multi-pass approach to large-scale connectomics, arXiv
  28. Karnovsky, 1965, A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy, J Cell Biol, 27, 1A
  29. Pallotto, 2015, Extracellular space preservation aids the connectomic analysis of neural circuits, eLife, 4, 10.7554/eLife.08206 · DOI
  30. Wakai, 2013, Mannitol for acute traumatic brain injury, Cochrane Database Syst Rev, 2013, CD001049
  31. Miyano, 2021, Hyperosmotic stress induces epithelial-mesenchymal transition through rearrangements of focal adhesions in tubular epithelial cells, PLoS One, 16, 10.1371/journal.pone.0261345 · DOI
  32. Rungta, 2015, The cellular mechanisms of neuronal swelling underlying cytotoxic edema, Cell, 161, 610, 10.1016/j.cell.2015.03.029 · DOI
  33. Hobro, 2017, An evaluation of fixation methods: Spatial and compositional cellular changes observed by Raman imaging, Vib Spectrosc, 91, 31, 10.1016/j.vibspec.2016.10.012 · DOI
  34. Forbes, 1977, Cytochemical staining procedures selective for sarcotubular systems of muscle: Modifications and applications, J Ultrastruct Res, 60, 306, 10.1016/S0022-5320(77)80016-6 · DOI
  35. Hepler, 1981, The structure of the endoplasmic reticulum revealed by osmium tetroxide-potassium ferricyanide staining, Eur J Cell Biol, 26, 102
  36. Hopwood, 1970, The reactions between formaldehyde, glutaraldehyde and osmium tetroxide, and their fixation effects o bovine serum albumin and on tissue blocks, Histochemie, 24, 50, 10.1007/BF00310003 · DOI
  37. Glausier, 2019, Factors affecting ultrastructural quality in the prefrontal cortex of the postmortem human brain, J Histochem Cytochem, 67, 185, 10.1369/0022155418819481 · DOI
  38. Sorra, 1998, Critical assessment of the involvement of perforations, spinules, and spine branching in hippocampal synapse formation, J Comp Neurol, 398, 225, 10.1002/(SICI)1096-9861(19980824)398:2<225::AID-CNE5>3.0.CO;2-2 · DOI
  39. Spacek, 2004, Trans-endocytosis via spinules in adult rat hippocampus, J Neurosci, 24, 4233, 10.1523/JNEUROSCI.0287-04.2004 · DOI
  40. Tao-Cheng, 2009, Rapid turnover of spinules at synaptic terminals, Neuroscience, 160, 42, 10.1016/j.neuroscience.2009.02.031 · DOI
  41. Petralia, 2018, Invaginating structures in mammalian synapses, Front Synaptic Neurosci, 10, 4, 10.3389/fnsyn.2018.00004 · DOI
  42. Petralia, 2021, Invaginating structures in synapses - Perspective, Front Synaptic Neurosci, 13, 10.3389/fnsyn.2021.685052 · DOI
  43. Wood, 2021, Cytonemes with complex geometries and composition extend into invaginations of target cells, J Cell Biol, 220, 10.1083/jcb.202101116 · DOI
  44. Makarov, 2015, On the role of the blood vessel endothelial microvilli in the blood flow in small capillaries, J Biophys, 2015
  45. Heuser, 1974, Functional changes in frog neuromuscular junctions studied with freeze-fracture, J Neurocytol, 3, 109, 10.1007/BF01111936 · DOI
  46. Heuser, 1979, Synaptic vesicle exocytosis captured by quick freezing and correlated with quantal transmitter release, J Cell Biol, 81, 275, 10.1083/jcb.81.2.275 · DOI
  47. Heuser, 1981, Structural changes after transmitter release at the frog neuromuscular junction, J Cell Biol, 88, 564, 10.1083/jcb.88.3.564 · DOI
  48. Heuser, 1989, Review of electron microscopic evidence favouring vesicle exocytosis as the structural basis for quantal release during synaptic transmission, Q J Exp Physiol, 74, 1051, 10.1113/expphysiol.1989.sp003333 · DOI
  49. Jung, 2018, Active zone material-directed orientation, docking, and fusion of dense core vesicles alongside synaptic vesicles at neuromuscular junctions, Front Neuroanat, 12, 72, 10.3389/fnana.2018.00072 · DOI
  50. White, 1976, The chemical nature of osmium tetroxide fixation and staining of membranes by x-ray photoelectron spectroscopy, Biochim Biophys Acta, 436, 577, 10.1016/0005-2736(76)90442-9 · DOI
  51. Rodriguez-Moreno, 2018, Quantitative 3D ultrastructure of thalamocortical synapses from the “lemniscal” ventral posteromedial nucleus in mouse barrel cortex, Cereb Cortex, 28, 3159, 10.1093/cercor/bhx187 · DOI
  52. Rollenhagen, 2020, Synaptic organization of the human temporal lobe neocortex as revealed by high-resolution transmission, focused ion beam scanning, and electron microscopic tomography, Int J Mol Sci, 21, 5558, 10.3390/ijms21155558 · DOI
  53. De Bruijn, 1975, Glycogen, its chemistry and morphological appearance in the electron microscope.11. The complex formed in the selective contrast staining of glycogen, Histochem J, 7, 205, 10.1007/BF01003591 · DOI
  54. de Bruijn, 1976, Glycogen, its chemistry and morphological appearance in the electron microscope. III. Identification of the tissue ligands involved in the glycogen contrast staining reaction with the osmium (VI)–iron(II) complex, Histochem J, 8, 121, 10.1007/BF01007164 · DOI
  55. Fiala, 2003, Timing of neuronal and glial ultrastructure disruption during brain slice preparation and recovery in vitro, J Comp Neurol, 465, 90, 10.1002/cne.10825 · DOI
  56. Bamisi, 2020, Effects of various fixatives and temperature on the quality of glycogen demonstration in the brain and liver tissues, Ann Diagn Pathol, 48, 10.1016/j.anndiagpath.2020.151604 · DOI
  57. Zakout, 2010, The effect of fixatives and temperature on the quality of glycogen demonstration [published correction appears in Biotech Histochem 2011;86:448], Biotech Histochem, 85, 93, 10.3109/10520290903126883 · DOI
  58. Ornelas, 2021, Three-dimensional ultrastructure of the brain pericyte-endothelial interface, J Cereb Blood Flow Metab, 41, 2185, 10.1177/0271678X211012836 · DOI
  59. Dejana, 2009, Organization and signaling of endothelial cell-to-cell junctions in various regions of the blood and lymphatic vascular trees, Cell Tissue Res, 335, 17, 10.1007/s00441-008-0694-5 · DOI
  60. Rosenmund, 1996, Definition of the readily releasable pool of vesicles at hippocampal synapses, Neuron, 16, 1197, 10.1016/S0896-6273(00)80146-4 · DOI

Cited by

Sorted by citations

See all 33

Metadata

Authors
Karlupia, N., Schalek, R. L., Wu, Y., Meirovitch, Y., Wei, D., Charney, A. W., Kopell, B. H., & Lichtman, J. W.
Venue
Biological Psychiatry
Publisher
Elsevier BV
Published
2023-08
Volume
94
Issue
4
Pages
352-360
ISSN
0006-3223
Cited by
33
References
4
Language
en
Funders
  • National Institute of Mental Health (P50MH094271)
  • National Institutes of Health (U19-NS104653, UG3-MH123386)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Immersion Fixation and Staining of Multicubic Millimeter Volumes for Electron Microscopy–Based Connectomics of Human Brain Biopsies. https://app.cochat.ai/papers/10.1016/j.biopsych.2023.01.025
The paper
Karlupia, N., Schalek, R. L., Wu, Y., Meirovitch, Y., Wei, D., Charney, A. W., Kopell, B. H., & Lichtman, J. W. (2023). Immersion Fixation and Staining of Multicubic Millimeter Volumes for Electron Microscopy–Based Connectomics of Human Brain Biopsies. Biological Psychiatry. https://doi.org/10.1016/j.biopsych.2023.01.025