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Cited by 138Enzyme-mediated dye degradationEnzyme Production and CharacterizationTannin, Tannase and Anticancer Activities

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Purification, biochemical characterization and dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens

Si, Jing, Peng, Feng, Cui, Baokai·2013·Bioresource Technology
DOI 10.1016/j.biortech.2012.10.085
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Abstract

Extracellular laccase (Tplac) from Trametes pubescens was purified to homogeneity by a three-step method, which resulted in a high specific activity of 18.543 Umg(-1), 16.016-fold greater than that of crude enzyme at the same level. Tplac is a monomeric protein that has a molecular mass of 68 kDa. The enzyme demonstrated high activity toward 1.0mM ABTS at an optimum pH of 5.0 and temperature of 50 °C, and under these conditions, the catalytic efficiency (k(cat)/K(m)) is 8.34 s(-1) μM(-1). Tplac is highly stable and resistant under alkaline conditions, with pH values ranging from 7.0 to 10.0. Interestingly, above 88% of initial enzyme activity was maintained in the presence of metal ions at 25.0mM, leading to an increase in substrate affinity, which indicated that the laccase is highly metal-tolerant. These unusual properties demonstrated that the new fungal laccase Tplac has potentials for the specific industrial or environmental applications.

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Metadata

Authors
Si, J., Peng, F., & Cui, B.
Affiliations
Beijing Forestry University (CN)
ORCID
Venue
Bioresource Technology
Publisher
Elsevier BV
Published
2013-01
Volume
128
Pages
49-57
ISSN
0960-8524 (ISSN-L)
DOI
10.1016/j.biortech.2012.10.085
OpenAlex
W1988614666
Cited by
138
References
0
Citations by year
2026: 3 · 2025: 7 · 2024: 12 · 2023: 11 · 2022: 6 · 2021: 15 · 2020: 10 · 2019: 17 · 2018: 14 · 2017: 12 · 2016: 8 · 2015: 6 · 2014: 12 · 2013: 5
FWCI
8.41
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Bioresource Technology (publishedVersion)
  • PubMed (publishedVersion)
Funders
Program for New Century Excellent Talents in University
Topics
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Enzyme-mediated dye degradation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Physical Sciences › Chemistry › Organic Chemistry › Tannin, Tannase and Anticancer Activities
Keywords
laccase, Trametes pubescens, fungal extracellular enzymes, enzyme purification, alkali resistance, metal tolerance, dye decolorization, ABTS, enzyme kinetics, enzyme stability, monomeric protein, industrial biocatalysis
MeSH terms
Alkalies, Color, Coloring Agents, Enzyme Stability, Metals, Water Pollutants, Chemical, Water Purification, Laccase, Trametes, Wastewater

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CoChat. (2026). Citation check: Purification, biochemical characterization and dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens. https://app.cochat.ai/papers/10.1016/j.biortech.2012.10.085
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Si, J., Peng, F., & Cui, B. (2013). Purification, biochemical characterization and dye decolorization capacity of an alkali-resistant and metal-tolerant laccase from Trametes pubescens. Bioresource Technology. https://doi.org/10.1016/j.biortech.2012.10.085