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Degradation of synthetic pollutants in real wastewater using laccase encapsulated in core–shell magnetic copper alginate beads
Abstract
Immobilization of laccase has been highlighted to enhance their stability and reusability in bioremediation. In this study, we provide a novel immobilization technique that is very suitable to real wastewater treatment. A perfect core-shell system composing copper alginate for the immobilization of laccase (Lac-beads) was produced. Additionally, nFe2O3 was incorporated for the bead recycling through magnetic force. The beads were proven to immobilize 85.5% of total laccase treated and also to be structurally stable in water, acetate buffer, and real wastewater. To test the Lac-beads reactivity, triclosan (TCS) and Remazol Brilliant Blue R (RBBR) were employed. The Lac-beads showed a high percentage of TCS removal (89.6%) after 8h and RBBR decolonization at a range from 54.2% to 75.8% after 4h. Remarkably, the pollutants removal efficacy of the Lac-beads was significantly maintained in real wastewater with the bead recyclability, whereas that of the corresponding free laccase was severely deteriorated.
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Le et al. 2021 found that immobilization of commercial T. versicolor laccase on copper alginate beads affected degradation of the contaminant between 16.7% and 34.0% compared to the free enzyme.
“Le et al., 2021 used copper alginate beads for commercial laccase from T. versicolor (Sigma-Aldrich) and found that immobilization affected the degradation of the containment agent between 16.7% and 34.0%, compared to the free enzyme [221].”
From A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases (2021) · cites this paper as [221]
In some cases, degradation of contaminants decreases when using immobilized enzyme.
“However, in some cases, the degradation of contaminants decreases when using the immobilized enzyme.”
From A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases (2021) · cites this paper as [221]
Removal of RBBR (artificially added) in real-wastewater was between 37.6% and 54.8% in 24 h, with variation due to different types of wastewaters used.
“The removal of RBBR (artificially added) in real-wastewater was between 37.6% and 54.8% in 24 h. The variation was due to the different types of wastewaters used [221].”
From A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases (2021) · cites this paper as [221]
Citations
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Transformation pathway of Remazol Brilliant Blue R by immobilised laccase
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A Brief History of Colour, the Environmental Impact of Synthetic Dyes and Removal by Using Laccases
Ardila-Leal, L. D., Poutou-Piñales, R. A., Pedroza-Rodríguez, A. M., & Quevedo-Hidalgo, B. E. (2021) · Molecules · doi:10.3390/molecules26133813 · Cited by 544
Metadata
- Authors
- Le, T. T., Murugesan, K., Lee, C.-S., Vu, C. H., Chang, Y.-S., & Jeon, J.-R.
- Affiliations
- Pohang University of Science and Technology (KR)
- Periyar University (IN)
- Gyeongsang National University (KR)
- Corresponding author
- Chang, Yoon-Seok
- Venue
- Bioresource Technology
- Publisher
- Elsevier BV
- Published
- 2016-09
- Volume
- 216
- Pages
- 203-210
- ISSN
- 0960-8524 (ISSN-L)
- OpenAlex
- W2395883883
- Cited by
- 133
- References
- 1
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 7 · 2023: 16 · 2022: 10 · 2021: 25 · 2020: 23 · 2019: 16 · 2018: 13 · 2017: 15 · 2016: 1
- FWCI
- 17.57
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Bioresource Technology (publishedVersion)
- PubMed (publishedVersion)
- Open Access System for Information Sharing (Pohang University of Science and Technology) (submittedVersion)
- Funders
- National Research Foundation of Korea (2011-0028723)
- Korea Ministry of Environment (RE201402059)
- Gyeongsang National University
- Topics
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Enzyme-mediated dye degradation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
- Physical Sciences › Environmental Science › Water Science and Technology › Adsorption and biosorption for pollutant removal
- Keywords
- laccase immobilization, magnetic iron oxide, enzyme reusability, triclosan degradation, Remazol Brilliant Blue R, dye decolorization, wastewater treatment, bioremediation, enzyme stability
- MeSH terms
- Alginates, Anthraquinones, Biodegradation, Environmental, Chemical Industry, Enzymes, Immobilized, Hexuronic Acids, Magnetics, Waste Disposal, Fluid, Water Pollutants, Chemical, Glucuronic Acid, Laccase, Wastewater
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