Label-free detection of 3-nitro-l-tyrosine with nickel-doped graphene localized surface plasmon resonance biosensor
Abstract
3-nitro-l-tyrosine (3-NT) is believed to be a biomarker of neurodegenerative diseases and metal doped graphene possess exceptionally high binding energy of 3-NT with metal-nitro chemisorption. Here we report a novel label-free detection scheme of 3-NT via nickel-doped graphene (NDG) as the functionalized receptor on our phase detecting localized surface plasmon resonance (LSPR) biosensor. When compared with reported 3-NT immunoassay with enzyme-linked immunosorbent assay (ELISA), our NDG-LSPR platform offers two advantages i.e. 1) label-free and 2) capture of 3-NT by direct chemisorption. Our limit of detection for 3-NT in PBS was found to be 0.13pg/ml and the linear dynamic range of response was from 0.5pg/ml to 1ng/ml, i.e. four orders of magnitude. The specificity of our NDG receptor to 3-NT was also verified with l-tyrosine of equivalent concentrations in PBS and diluted human serum, for which the NDG receptor shows negligible responses. In addition, the adsorption of 3-NT and l-tyrosine to the NDG receptor were also investigated by atomic force microscopy and further verified by surface enhanced Raman spectroscopy. Therefore, our NDG-LSPR biosensor competes favorably against ELISA and we believe it should be an attractive and economical solution to early diagnostic of 3-NT related disorders for clinical applications.
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In 2016, Ng et al. demonstrated a novel detection scheme of 3-NT using a nickel-doped graphene synthetic receptor combined with a localized surface plasmon resonance biosensor.
“In 2016, Ng et al. demonstrated a novel detection scheme of 3-NT by using a nickel-doped graphene synthetic receptor combined with a localized surface plasmon resonance biosensor”
From Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases (2021) · cites this paper as [200]
Cites
Kinetic analysis of macromolecular interactions using surface plasmon resonance biosensors
Myszka, D. G. (1997) · Current Opinion in Biotechnology · doi:10.1016/s0958-1669(97)80157-7 · Cited by 552 · see citing works
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Protein oxidation - Formation mechanisms, detection and relevance as biomarkers in human diseases
Kehm, R., Baldensperger, T., Raupbach, J., & Höhn, A. (2021) · Redox Biology · doi:10.1016/j.redox.2021.101901 · Cited by 348 · see citing works
“As declared by the authors, this approach provides advantages compared to other techniques, such as immunoassays, due to label-free detection, higher specificity and capture of 3-NT by direct chemisorption [200].”
Result
- Authors
- Ng, S. P., Qiu, G., Ding, N., Lu, X., & Wu, C.-M. L.
- Affiliations
- City University of Hong Kong (HK)
- Corresponding author
- Wu, Chi-Man Lawrence
- Venue
- Biosensors and Bioelectronics
- Publisher
- Elsevier BV
- Published
- 2017-03
- Volume
- 89
- Issue
- Pt 1
- Pages
- 468-476
- ISSN
- 0956-5663 (ISSN-L)
- OpenAlex
- W2326867847
- Cited by
- 55
- References
- 1
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 4 · 2023: 6 · 2022: 8 · 2021: 9 · 2020: 6 · 2019: 7 · 2018: 5 · 2017: 4 · 2016: 1
- FWCI
- 2.73
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Biosensors and Bioelectronics (publishedVersion)
- PubMed (publishedVersion)
- Funders
- City University of Hong Kong (Grant No. 9667110)
- National Natural Science Foundation of China (Grant No. 11404192)
- Shandong Province Special Grant for High-Level Overseas Talents (tshw20120745)
- Outstanding Young and Middle-aged Scientists of Shandong Province (BS2014CL002)
- Topics
- Physical Sciences › Engineering › Biomedical Engineering › Plasmonic and Surface Plasmon Research
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
- Physical Sciences › Engineering › Electrical and Electronic Engineering › Electrochemical sensors and biosensors
- Keywords
- nitrotyrosine, label-free detection, chemisorption, neurodegenerative disease biomarkers, metal-doped graphene, tyrosine, human serum, early diagnosis
- MeSH terms
- Enzyme-Linked Immunosorbent Assay, Gold, Graphite, Humans, Nickel, Tyrosine, Biomarkers, Neurodegenerative Diseases, Surface Plasmon Resonance, Nanostructures, Limit of Detection
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