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Cited by 61Biosensors and Analytical DetectionAdvanced Biosensing Techniques and ApplicationsPhotonic and Optical Devices

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Guided mode resonance sensor for the parallel detection of multiple protein biomarkers in human urine with high sensitivity

Open access
Kenaan, Ahmad, Li, Kezheng, Barth, Isabel, Johnson, Steven, Song, Jie, Krauss, Thomas F.·2020·Biosensors and Bioelectronics
DOI 10.1016/j.bios.2020.112047
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Abstract

The rising cost of global healthcare provision and new approaches to managing disease are driving the development of low-cost biosensing modalities, such as label-free photonic methods based on dielectric resonances. Here, we use the combined sensing and imaging capability of a guided mode resonance (GMR) sensor to detect multiple biomarkers (troponin, procalcitonin and C-Reactive Protein) in parallel in undiluted urine samples. A key requirement of such a biosensor is the simple and direct functionalization with suitable antibodies to ensure the disease-specific detection of protein biomarkers. Here, antibodies were immobilized using a succinimidyl-[(N-maleimidopropionamido)-hexaethyleneglycol] ester (SM(PEG)6) spacer. The polyethylene glycol (PEG) chemistry enables low detection limits of 10 pg mL-1 or better for all protein biomarkers, while minimizing non-specific binding compared to more commonly used strategies such as (3-Aminopropyl)triethoxysilane (APTES) or dextran. Our approach supports the vision of a simple yet highly sensitive diagnostic platform that could be used for pre-screening patients for a wide range of diseases at point-of-care, thereby relieving the pressure on overstretched healthcare services.

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References 32 · Cited by 61

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Metadata

Authors
Kenaan, A., Li, K., Barth, I., Johnson, S., Song, J., & Krauss, T. F.
Affiliations
  • Shanghai Jiao Tong University (CN)
  • University of York (GB)
Corresponding author
Krauss, Thomas F.
ORCID
Venue
Biosensors and Bioelectronics
Publisher
Elsevier BV
Published
2020-04
Volume
153
Pages
112047
ISSN
0956-5663 (ISSN-L)
DOI
10.1016/j.bios.2020.112047
OpenAlex
W3002922055
Cited by
61
References
32
Citations by year
2026: 7 · 2025: 14 · 2024: 9 · 2023: 15 · 2022: 4 · 2021: 7 · 2020: 5
FWCI
2.44
Type
journal article
Language
en
Open access
Licence
Locations
  • Biosensors and Bioelectronics (publishedVersion)
  • PubMed (publishedVersion)
  • White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (submittedVersion)
Funders
Engineering and Physical Sciences Research Council
Topics
  • Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
  • Physical Sciences › Engineering › Electrical and Electronic Engineering › Photonic and Optical Devices
Keywords
label-free biosensing, protein biomarkers, troponin, procalcitonin, C-reactive protein, antibody immobilization, PEG spacer, dielectric resonance, point-of-care testing, multiplexed detection, non-specific binding reduction
MeSH terms
Humans, Immunoassay, Polyethylene Glycols, Propylamines, Sensitivity and Specificity, Silanes, Surface Properties, Biomarkers, Silicon Compounds, Point-of-Care Systems, Surface Plasmon Resonance, Nanostructures, Antibodies, Immobilized, Limit of Detection, Quartz Crystal Microbalance Techniques

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