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Exploring the fundamental limit of antimicrobial susceptibility by near-single-cell electrical impedance spectroscopy
Open accessAbstract
Many novel susceptibility tests are being developed to tackle the worldwide problem of antimicrobial resistance (AMR). The key driver behind these developments, that is the need to reduce the response time, requires an understanding of which bacterial characteristic needs to be monitored to provide a rapid and ideally universal signature of susceptibility. Many characteristics have already been studied, most notably bacterial growth, metabolism and motility. Here, we consider electrical impedance to directly access bacterial metabolism, which can be considered a fundamental indicator of bacterial viability. By studying the electrical response of individual bacteria to an antibiotic challenge, we detect antimicrobial action close to its biological limit. Specifically, we find that it takes 30-60 min to register significant changes in impedance for clinical concentrations of antibiotics, in line with other rapid indicators. Our findings suggest that 60 min is the fundamental lower limit of response time for a realistic susceptibility test at clinically relevant antibiotic concentrations.
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“The impedance-based assay is a powerful technique that can detect alterations in the electrical properties of microbial cells in response to interactions with antimicrobial agents [95].”
From Methods for screening and evaluation of antimicrobial activity: A review of protocols, advantages, and limitations (2024) · cites this paper as [95]
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Cited by
Sorted by citations
Hossain, T. J. (2024) · European Journal of Microbiology and Immunology · doi:10.1556/1886.2024.00035 · Cited by 392
Metadata
- Authors
- Pitruzzello, G., Johnson, S., & Krauss, T. F.
- Affiliations
- University of York (GB)
- Corresponding author
- Pitruzzello, Giampaolo
- Krauss, Thomas F.
- Venue
- Biosensors and Bioelectronics
- Publisher
- Elsevier BV
- Published
- 2023-03
- Volume
- 224
- Pages
- 115056
- ISSN
- 0956-5663 (ISSN-L)
- OpenAlex
- W4313368492
- Cited by
- 20
- References
- 2
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 10 · 2023: 3
- FWCI
- 2.78
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Biosensors and Bioelectronics (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- Engineering and Physical Sciences Research Council (EP/P02324X/1, EP/P030017/1)
- Royal Society
- Topics
- Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
- Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Antimicrobial agents and applications
- Physical Sciences › Chemistry › Analytical Chemistry › Electrochemical sensors and biosensors
- Keywords
- antimicrobial susceptibility testing, electrical impedance spectroscopy, single-cell analysis, bacterial metabolism, bacterial viability, antimicrobial resistance
- MeSH terms
- Anti-Bacterial Agents, Bacteria, Microbial Sensitivity Tests, Biosensing Techniques, Dielectric Spectroscopy
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