Resonant Reflection Spectroscopy of Biomolecular Arrays in Muscle
Open accessAbstract
Sarcomeres, the functional units of contraction in striated muscle, are composed of an array of interdigitating protein filaments. Direct interaction between overlapping filaments generates muscular force, which produces animal movement. When filament length is known, sarcomere length successfully predicts potential force, even in whole muscles that contain billions of sarcomere units. Inability to perform in vivo sarcomere measurements with submicrometer resolution is a long-standing challenge in the muscle physiology field and has hampered studies of normal muscle function, adaptation, injury, aging, and disease, particularly in humans. Here, we develop theory and demonstrate the feasibility of to our knowledge a new technique that measures sarcomere length with submicrometer resolution. In this believed novel approach, we examine sarcomere structure by measuring the multiple resonant reflections that are uniquely defined by Fourier decomposition of the sarcomere protein spatial framework. Using a new supercontinuum spectroscopic system, we show close agreement between sarcomere lengths measured by resonant reflection spectroscopy and laser diffraction in an ensemble of 10 distinct muscles.
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The muscle fiber can be considered as a grating structure of regularly separated bands.
“Since both the electron microscope and SAXS results show that the muscle fiber is highly ordered along the axial direction, and the periodicity is decided by the band structure of the sarcomere, thus it can be considered as a grating structure of regularly separated bands (31)”
From Light diffraction by sarcomeres produces iridescence in transmission in the transparent ghost catfish (2023) · cites this paper as [31]
The difference in density generates a periodic variation in refractive index along the axial direction of the myofibrils.
“The difference in density thus generates a periodic variation in refractive index along the axial direction of the myofibrils (Fig. 3B) (31).”
From Light diffraction by sarcomeres produces iridescence in transmission in the transparent ghost catfish (2023) · cites this paper as [31]
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STATISTICAL METHODS FOR ASSESSING AGREEMENT BETWEEN TWO METHODS OF CLINICAL MEASUREMENT
Martin Bland, J., & Altman, D. (1986) · The Lancet · doi:10.1016/s0140-6736(86)90837-8 · Cited by 47,965 · see citing works
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- [22]22. Born, M., and E. Wolf. 1999. Principles of Optics: Electromagnetic Theory of Propagation, Interference and Diffraction of Light. Cambridge University Press, Cambridge, United Kingdom.
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Fan, X., Zheng, X., An, T., Li, X., Leung, N., Zhu, B., Sui, T., Shi, N., Fan, T., & Zhao, Q. (2023) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2219300120 · Cited by 11 · see citing works
- Authors
- Young, K. W., Radic, S., Myslivets, E., O’Connor, S. M., & Lieber, R. L.
- Affiliations
- University of California San Diego (US)
- Shirley Ryan AbilityLab (US)
- Corresponding author
- Lieber, Richard L.
- Venue
- Biophysical Journal
- Publisher
- Elsevier BV
- Published
- 2014-11
- Volume
- 107
- Issue
- 10
- Pages
- 2352-2360
- ISSN
- 0006-3495 (ISSN-L)
- PMC
- PMC4241457
- OpenAlex
- W2075447776
- Cited by
- 4
- References
- 1
- Citations by year
- 2023: 1 · 2021: 1 · 2017: 2
- FWCI
- 0.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Biophysical Journal (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R24HD050837)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Keywords
- sarcomere length, sarcomere structure, striated muscle, Fourier decomposition, submicron resolution, muscle contraction
- MeSH terms
- Animals, Feasibility Studies, Models, Biological, Muscle Proteins, Rabbits, Sarcomeres, Spectrum Analysis, Mice, Optical Phenomena
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