Patient-derived organoids in translational oncology and drug screening
Open accessAbstract
Patient-derived organoids (PDO) are a new biomedical research model that can reconstruct phenotypic and genetic characteristics of the original tissue and are useful for research on pathogenesis and drug screening. To introduce the progression in this field, we review the key factors of constructing organoids derived from epithelial tissues and cancers, covering culture medium and matrix, morphological characteristics, genetic profiles, high-throughput drug screening, and application potential. We also discuss the co-culture system of cancer organoids with tumor microenvironment (TME) associated cells. The co-culture system is widely used in evaluating crosstalk of cancer cells with TME components, such as fibroblasts, endothelial cells, immune cells, and microorganisms. The article provides a prospective for standardized cultivation mode, automatic morphological evaluation, and drug sensitivity screening using high-throughput methods.
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Cancer organoids maintain the physiological structure and function of their source tissue and are widely used in research on tumor pathogenesis, drug development, treatment resistance, and other fields.
“Cancer organoids, which maintain the physiological structure and function of their source tissue, are widely used in research on tumor pathogenesis, drug development, treatment resistance, and other fields48,49.”
From An HDAC4-specific PROTAC degrader achieves radiation sensitization by enhancing ferroptosis in lung cancer (2026) · cites this paper as [49]
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Cheng, C., Sun, L., Yang, J., Liu, Z., Zhang, F., Qiu, L., Yu, M., Wang, T., Huang, J., Wang, L., et al. (2026) · Nature Communications · doi:10.1038/s41467-026-73682-0
- Authors
- Yang, R., & Yu, Y.
- Affiliations
- Shanghai Jiao Tong University (CN)
- Corresponding author
- Yu, Yingyan
- Venue
- Cancer Letters
- Publisher
- Elsevier BV
- Published
- 2023-05
- Volume
- 562
- Pages
- 216180
- ISSN
- 0304-3835 (ISSN-L)
- OpenAlex
- W4365458878
- Cited by
- 99
- References
- 8
- Citations by year
- 2026: 21 · 2025: 28 · 2024: 44 · 2023: 6
- FWCI
- 3.22
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Cancer Letters (publishedVersion, cc-by-nc)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Physical Sciences › Mathematics › Modeling and Simulation › Mathematical Biology Tumor Growth
- Keywords
- patient-derived organoids, tumor organoids, organoid morphology, high-throughput drug screening, drug sensitivity, tumor microenvironment, co-culture model, cancer-associated fibroblasts, endothelial cells, immune cells, microbiota
- MeSH terms
- Drug Evaluation, Preclinical, Humans, Neoplasms, Organoids, Prospective Studies, Endothelial Cells, Tumor Microenvironment
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