The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis
Abstract
The polysaccharides (PKP-E) extracted from the pinecones of Pinus koraiensis were studied, which was fractionated using DEAE-52 cellulose and Sephadex G-100. Four novel polysaccharide fractions were obtained, which were PKP-E-1-1, -1-2, -2-1, and -2-2, respectively. The structural features were characterized using HPGPC, monosaccharide composition analysis, Congo red test, periodate oxidation, Smith degradation, FTIR and NMR spectroscopy. The results showed the 4 purified fractions were non-triple helical structured heteropolysaccharides and composed of l-rhamnose, l-arabinose, d-mannose, d-glucose, and d-galactose. The fractions were mainly linked by 1→6 or 1→ glycosidic bonds and the backbone of 4 fractions was probably composed of→2, 6)-β-d-Man-(1→ and α-d-GalpA-(1→), which resembles pectin. Moreover, the antioxidant activities of the polysaccharides were measured by scavenging radical capacity tests. The PKP-E-2-1 was the most stable and active fraction, and the respective IC50 for the hydroxyl and ABTS·+ radicals were 3.0 and 23.6 mg/mL.
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Metadata
- Authors
- Zhang, H., Zou, P., Zhao, H., Qiu, J., Regenstein, J. M., & Yang, X.
- Venue
- Carbohydrate Polymers
- Publisher
- Elsevier BV
- Published
- 2021-01
- Volume
- 251
- Pages
- 117078
- ISSN
- 0144-8617 (ISSN-L)
- OpenAlex
- W3085547007
- Cited by
- 258
- References
- 29
- FWCI
- 15.50
- Type
- journal article
- Language
- en
- Open access
- closed
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- Zhang, H., Zou, P., Zhao, H., Qiu, J., Regenstein, J. M., & Yang, X. (2021). Isolation, purification, structure and antioxidant activity of polysaccharide from pinecones of Pinus koraiensis. Carbohydrate Polymers. https://doi.org/10.1016/j.carbpol.2020.117078
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