The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Tests for the mechanism of starch biosynthesis: de novo synthesis or an amylogenin primer synthesis
Abstract
Studies in 1940 on potato phosphorylase reaction with starch found that d-glucopyranose from α-d-glucopyranosyl-1-phosphate was added to the nonreducing-ends of starch chains. This led to the hypothesis that the biosynthesis of starch required a preformed primer. Later it was found that phosphorylase was exclusively a degradative enzyme in vivo and that starch-synthase was the enzyme that reacted with ADPGlc to biosynthesize starch. Amylogenin, a putative self-glycosylated protein, was postulated to be the primer, although it was never demonstrated or found. In the present study, three reactions were performed in sequence with a highly purified potato starch-synthase to determine whether an amylogenin primer was present and required or whether the biosynthesis was de novo. Reaction 1 was performed by adding 2.0mM ADPGlc to synthesize the putative primer to a possible amylogenin in the preparation; in Reaction 2, 10mM ADP-[(14)C]Glc was added; and in Reaction 3, 10mM nonlabeled ADPGlc was added. After the isolation, reduction, and acid hydrolysis of the products of Reactions 2 and 3, (14)C-d-glucitol was obtained from Reaction 2 and was decreased by Reaction 3. The formation of (14)C-d-glucitol and its decrease showed that an amylogenin, protein primer was not involved in starch biosynthesis and the synthesis is de novo by the addition of d-glucose to the reducing-ends of growing starch chains.
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“In contrast, the mechanism of potato starch biosynthesis appeared to involve de novo synthesis, not an amylogenin primer (568).”
From Biological roles of glycans (2017) · cites this paper as [568]
Citations
Cites
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Cited by
Sorted by citations
Varki, A. (2017) · Glycobiology · doi:10.1093/glycob/cww086 · Cited by 2,635
“In contrast, the mechanism of potato starch biosynthesis appeared to involve de novo synthesis, not an amylogenin primer (568).”
Background
Metadata
- Authors
- Mukerjea, R., & Robyt, J. F.
- Affiliations
- Iowa State University (US)
- Venue
- Carbohydrate Research
- Publisher
- Elsevier BV
- Published
- 2013-05
- Volume
- 372
- Pages
- 55-59
- ISSN
- 0008-6215 (ISSN-L)
- OpenAlex
- W1995590464
- Cited by
- 4
- References
- 26
- Citations by year
- 2025: 1 · 2021: 1 · 2016: 1 · 2013: 1
- FWCI
- 0.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Carbohydrate Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Glycogen Storage Diseases and Myoclonus
- Health Sciences › Nursing › Nutrition and Dietetics › Food composition and properties
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Polysaccharides and Plant Cell Walls
- Keywords
- starch biosynthesis, ADP-glucose, starch phosphorylase, potato
- MeSH terms
- Glucose, Glycoproteins, Hydrolysis, Plant Proteins, Solanum tuberosum, Starch, Starch Synthase, Intramolecular Transferases
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