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Cited by 44Liver physiology and pathologyDrug-Induced Hepatotoxicity and ProtectionPlant-derived Lignans Synthesis and Bioactivity

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Transcriptomic analyses reveal the molecular mechanisms of schisandrin B alleviates CCl4-induced liver fibrosis in rats by RNA-sequencing

Zhang, Hai, Chen, Qingshan, Dahan, Arik, Xue, Jiyang, Wei, Liwen, Tan, Weifeng, Zhang, Guoqing·2019·Chemico-Biological Interactions
DOI 10.1016/j.cbi.2019.05.041
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Abstract

Liver fibrosis is a progression of chronic liver disease with lacks effective therapies at present. Schisandrin B (Sch B), a bioactive compound extracted from the traditional Chinese medicine Schisandra chinensis, was reported to benefit liver diseases. This study aimed to investigate the therapeutic effects and molecular mechanisms of Sch B against CCl 4 -induced liver fibrosis in rats. RNA sequencing and transcriptome analysis were performed collaboratively, including analysis of differential gene expression, gene ontology (GO) analysis, pathway analysis and pathway-act-network analysis. The results demonstrated that Sch B effectively alleviated CCl 4 -induced liver damage and fibrosis in rats, as evidenced by improved liver function and decreased extracellular matrix deposition. Furthermore, 4440 (1878 up-regulated, 2562 down-regulated) genes in the model group versus (vs) normal group, 4243 (2584 up-regulated, 1659 down-regulated) genes in Sch B-treated group vs model group were identified as differentially expressed genes (DEGs). Subsequently, GO analysis revealed that DEGs were mainly enriched in metabolism, oxidation-reduction, endoplasmic reticulum stress and apoptosis-related biological processes. Pathway analysis suggested that Sch B up-regulated cytochrome P450 drug metabolism, PPAR signaling pathways, and down-regulated glutathione metabolism pathways. In addition, the regulatory patterns of Sch B on key genes and pathways were also confirmed. In conclusion, our s…

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Metadata

Authors
Zhang, H., Chen, Q., Dahan, A., Xue, J., Wei, L., Tan, W., & Zhang, G.
Affiliations
  • Tongji University (CN)
  • Second Military Medical University (CN)
  • Ben-Gurion University of the Negev (IL)
Corresponding author
  • Zhang, Hai
  • Tan, Weifeng
  • Zhang, Guoqing
ORCID
Venue
Chemico-Biological Interactions
Publisher
Elsevier BV
Published
2019-08
Volume
309
Pages
108675
ISSN
0009-2797 (ISSN-L)
DOI
10.1016/j.cbi.2019.05.041
OpenAlex
W2946874767
Cited by
44
References
1
Citations by year
2026: 7 · 2025: 9 · 2024: 4 · 2023: 7 · 2022: 7 · 2021: 6 · 2020: 3 · 2019: 1
FWCI
2.71
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Chemico-Biological Interactions (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (81773797)
  • Fundamental Research Funds for the Central Universities (1515219051)
  • Second Military Medical University (2018QN17)
Topics
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant-derived Lignans Synthesis and Bioactivity
Keywords
schisandrin B, liver fibrosis, carbon tetrachloride, Schisandra chinensis, differentially expressed genes, extracellular matrix deposition, endoplasmic reticulum stress, apoptosis, PPAR signaling, glutathione metabolism, hepatoprotection, traditional Chinese medicine
MeSH terms
Animals, Carbon Tetrachloride, Liver, Liver Cirrhosis, Male, Medicine, Chinese Traditional, Polycyclic Compounds, RNA, Signal Transduction, Down-Regulation, Up-Regulation, Rats, Wistar, Apoptosis, Sequence Analysis, RNA, Lignans, Gene Expression Profiling, Schisandra, Cyclooctanes, Rats, Transcriptome, Endoplasmic Reticulum Stress

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