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Directed enzyme evolution: climbing fitness peaks one amino acid at a time
Open accessAbstract
Directed evolution can generate a remarkable range of new enzyme properties. Alternate substrate specificities and reaction selectivities are readily accessible in enzymes from families that are naturally functionally diverse. Activities on new substrates can be obtained by improving variants with broadened specificities or by step-wise evolution through a sequence of more and more challenging substrates. Evolution of highly specific enzymes has been demonstrated, even with positive selection alone. It is apparent that many solutions exist for any given problem, and there are often many paths that lead uphill, one step at a time.
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Cites
Enzyme promiscuity: evolutionary and mechanistic aspects
Khersonsky, O., Roodveldt, C., & Tawfik, D. S. (2006) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2006.08.011 · Cited by 641
Cited by
Directed evolution drives the next generation of biocatalysts
Turner, N. J. (2009) · Nature Chemical Biology · doi:10.1038/nchembio.203 · Cited by 785
Enzymes and Bioconversions of Industrial, Pharmaceutical, and Biotechnological Significance
Sanchez, S., & Demain, A. L. (2011) · Organic Process Research & Development · doi:10.1021/op100302x · Cited by 189
Weber, N., Halpin, C., Hannah, L. C., Jez, J. M., Kough, J., & Parrott, W. (2012) · Plant Physiology · doi:10.1104/pp.112.204271 · Cited by 78
Explorations and Applications of Enzyme-linked Bioremediation of Synthetic Dyes
Ogola, H. J. O., Ashida, H., Ishikawa, T., & Sawa, Y. (2015) · Advances in Bioremediation of Wastewater and Polluted Soil · doi:10.5772/60753 · Cited by 5
Metadata
- Authors
- Tracewell, C. A., & Arnold, F. H.
- Venue
- Current Opinion in Chemical Biology
- Publisher
- Elsevier BV
- Published
- 2009-02
- Volume
- 13
- Issue
- 1
- Pages
- 3-9
- ISSN
- 1367-5931 (ISSN-L)
- OpenAlex
- W2059850101
- Cited by
- 321
- References
- 1
- FWCI
- 1.70
- Type
- journal article
- Language
- en
- Open access
- green
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