Various calibration procedures result in optimal standardization of routinely used 25(OH)D ID-LC-MS/MS methods
Open accessAbstract
Background The variety of LC-MS/MS methods measuring total 25(OH)D used today is vast and the comparability among these methods is still not well assessed. Methods Here, we performed a comparison in samples of healthy donors between the currently routinely used 25(OH)D LC-MS/MS methods in the Netherlands and the Ghent University reference measurement procedure to address this issue (n=40). Additionally, an interlaboratory comparison in patient serum samples assessed agreement between the Dutch diagnostic methods (n=37). Results The overall correlation of the routine methods for 25(OH)D 3 with the reference measurement procedures and with the mean of all diagnostic methods was excellent (r>0.993 and r>0.989, respectively). Three out of five methods aligned perfectly with both the reference measurement procedure and the median of all methods. One of the routine methods showed a small positive bias, while another showed a small negative bias consistently in both comparisons. Conclusion The biases most probably originated from differences in calibration procedure and may be obviated by reassessing calibration of stock standards and/or calibrator matrices. In conclusion, five diagnostic centers have performed a comparison with the 25(OH)D Ghent University reference measurement procedure in healthy donor serum samples and a comparison among themselves in patient serum samples. Both analyses showed a high correlation and specificity of the routine LC-MS/MS methods, yet did reveal some small standardization issues that could not be traced back to the technical details of the different methods. Hence, this study indicates various calibration procedures can result in perfect alignment.
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Serum 25(OH)D and total testosterone were measured by isotope-dilution liquid chromatography-tandem mass spectrometry and used for statistical analyses.
“Serum levels of 25(OH)D and TT were additionally measured by well-adjusted isotope-dilution liquid chromatography–tandem mass spectrometry (ID-LC–MS/MS) methods in 2018 [18, 19]. 25(OH)D and TT measured by ID-LC–MS/MS were used for statistical analyses.”
From Effects of vitamin D supplementation on androgens in men with low testosterone levels: a randomized controlled trial (2019) · cites this paper as [18]
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Lerchbaum, E., Trummer, C., Theiler-Schwetz, V., Kollmann, M., Wölfler, M., Heijboer, A. C., Pilz, S., & Obermayer-Pietsch, B. (2019) · European Journal of Nutrition · doi:10.1007/s00394-018-1858-z · Cited by 40 · see citing works
Self-citationFanelli, F., Cantù, M., Temchenko, A., Mezzullo, M., Lindner, J. M., Peitzsch, M., Hawley, J. M., Bruce, S., Binz, P.-A., Ackermans, M. T., et al. (2022) · Clinical Chemistry and Laboratory Medicine (CCLM) · doi:10.1515/cclm-2021-1028 · Cited by 36 · see citing works
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- Authors
- Dirks, N. F., Vesper, H. W., van Herwaarden, A. E., van den Ouweland, J. M. W., Kema, I. P., Krabbe, J. G., & Heijboer, A. C.
- Affiliations
- Amsterdam UMC Location Vrije Universiteit Amsterdam (NL)
- Centers for Disease Control and Prevention (US)
- University Medical Center Groningen (NL)
- Medisch Spectrum Twente (NL)
- Corresponding author
- Heijboer, Annemieke C.
- Venue
- Clinica Chimica Acta
- Publisher
- Elsevier BV
- Published
- 2016-11
- Volume
- 462
- Pages
- 49-54
- ISSN
- 0009-8981 (ISSN-L)
- PMC
- PMC5703036
- OpenAlex
- W2511618950
- Cited by
- 40
- References
- 0
- Citations by year
- 2025: 1 · 2024: 2 · 2023: 7 · 2022: 4 · 2021: 6 · 2020: 3 · 2019: 6 · 2018: 8 · 2017: 3
- FWCI
- 3.88
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Clinica Chimica Acta (publishedVersion)
- PubMed (publishedVersion)
- Amsterdam UMC (VU Amsterdam) - Institutional Repository (publishedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (publishedVersion)
- PubMed Central (acceptedVersion)
- Pure Amsterdam UMC (submittedVersion, other-oa)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Radboud Repository (Radboud University) (submittedVersion)
- University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
- Funders
- Dutch Foundation for Quality Assessment in Medical Laboratories
- Topics
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Pathology and Forensic Medicine › Vitamin D Research Studies
- Social Sciences › Decision Sciences › Statistics, Probability and Uncertainty › Scientific Measurement and Uncertainty Evaluation
- Keywords
- 25-hydroxyvitamin D, LC-MS/MS, method standardization, calibration method, interlaboratory comparison, reference measurement procedure, measurement bias
- MeSH terms
- Calcifediol, Calibration, Chromatography, Liquid, Humans, 25-Hydroxyvitamin D 2, Tandem Mass Spectrometry, Healthy Volunteers
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