The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Cohesin’s DNA Exit Gate Is Distinct from Its Entrance Gate and Is Regulated by Acetylation
Open accessAbstract
Sister chromatid cohesion is mediated by entrapment of sister DNAs by a tripartite ring composed of cohesin's Smc1, Smc3, and α-kleisin subunits. Cohesion requires acetylation of Smc3 by Eco1, whose role is to counteract an inhibitory (antiestablishment) activity associated with cohesin's Wapl subunit. We show that mutations abrogating antiestablishment activity also reduce turnover of cohesin on pericentric chromatin. Our results reveal a "releasing" activity inherent to cohesin complexes transiently associated with Wapl that catalyzes their dissociation from chromosomes. Fusion of Smc3's nucleotide binding domain to α-kleisin's N-terminal domain also reduces cohesin turnover within pericentric chromatin and permits establishment of Wapl-resistant cohesion in the absence of Eco1. We suggest that releasing activity opens the Smc3/α-kleisin interface, creating a DNA exit gate distinct from its proposed entry gate at the Smc1/3 interface. According to this notion, the function of Smc3 acetylation is to block its dissociation from α-kleisin. The functional implications of regulated ring opening are discussed.
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Cites
Mediator and cohesin connect gene expression and chromatin architecture
Kagey, M. H., Newman, J. J., Bilodeau, S., Zhan, Y., Orlando, D. A., van Berkum, N. L., Ebmeier, C. C., Goossens, J., Rahl, P. B., Levine, S. S., et al. (2010) · Nature · doi:10.1038/nature09380 · Cited by 1,939
Cohesins: Chromosomal Proteins that Prevent Premature Separation of Sister Chromatids
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Self-citationCohesin mediates transcriptional insulation by CCCTC-binding factor
Wendt, K. S., Yoshida, K., Itoh, T., Bando, M., Koch, B., Schirghuber, E., Tsutsumi, S., Nagae, G., Ishihara, K., Mishiro, T., et al. (2008) · Nature · doi:10.1038/nature06634 · Cited by 1,193
Sister-chromatid separation at anaphase onset is promoted by cleavage of the cohesin subunit Scc1
Uhlmann, F., Lottspeich, F., & Nasmyth, K. (1999) · Nature · doi:10.1038/21831 · Cited by 943
Self-citationCohesins Functionally Associate with CTCF on Mammalian Chromosome Arms
Parelho, V., Hadjur, S., Spivakov, M., Leleu, M., Sauer, S., Gregson, H. C., Jarmuz, A., Canzonetta, C., Webster, Z., Nesterova, T., et al. (2008) · Cell · doi:10.1016/j.cell.2008.01.011 · Cited by 900
Guacci, V., Koshland, D., & Strunnikov, A. (1997) · Cell · doi:10.1016/s0092-8674(01)80008-8 · Cited by 828
Cleavage of Cohesin by the CD Clan Protease Separin Triggers Anaphase in Yeast
Uhlmann, F., Wernic, D., Poupart, M.-A., Koonin, E. V., & Nasmyth, K. (2000) · Cell · doi:10.1016/s0092-8674(00)00130-6 · Cited by 798
Self-citationMultisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication
Nash, P., Tang, X., Orlicky, S., Chen, Q., Gertler, F. B., Mendenhall, M. D., Sicheri, F., Pawson, T., & Tyers, M. (2001) · Nature · doi:10.1038/35107009 · Cited by 759
Cohesin’s binding to chromosomes depends on a separate complex consisting of Scc2 and Scc4 proteins
Ciosk, R., Shirayama, M., Shevchenko, A., Tanaka, T., Toth, A., Shevchenko, A., & Nasmyth, K. (2000) · Molecular Cell · doi:10.1016/s1097-2765(00)80420-7 · Cited by 755
Self-citationMolecular Architecture of SMC Proteins and the Yeast Cohesin Complex
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Cited by
Wutz, G., Várnai, C., Nagasaka, K., Cisneros, D. A., Stocsits, R. R., Tang, W., Schoenfelder, S., Jessberger, G., Muhar, M., Hossain, M. J., et al. (2017) · The EMBO Journal · doi:10.15252/embj.201798004 · Cited by 864
The structural basis for cohesin–CTCF-anchored loops
Li, Y., Haarhuis, J. H. I., Sedeño Cacciatore, Á., Oldenkamp, R., van Ruiten, M. S., Willems, L., Teunissen, H., Muir, K. W., de Wit, E., Rowland, B. D., et al. (2020) · Nature · doi:10.1038/s41586-019-1910-z · Cited by 459
Wapl is an essential regulator of chromatin structure and chromosome segregation
Tedeschi, A., Wutz, G., Huet, S., Jaritz, M., Wuensche, A., Schirghuber, E., Davidson, I. F., Tang, W., Cisneros, D. A., Bhaskara, V., et al. (2013) · Nature · doi:10.1038/nature12471 · Cited by 359
WAPL maintains a cohesin loading cycle to preserve cell-type-specific distal gene regulation
Liu, N. Q., Maresca, M., van den Brand, T., Braccioli, L., Schijns, M. M. G. A., Teunissen, H., Bruneau, B. G., Nora, E. P., & de Wit, E. (2021) · Nature Genetics · doi:10.1038/s41588-020-00744-4 · Cited by 162
Cohesin and its regulation: on the logic of X-shaped chromosomes
Haarhuis, J. H. I., Elbatsh, A. M. O., & Rowland, B. D. (2014) · Developmental Cell · doi:10.1016/j.devcel.2014.09.010 · Cited by 147
Metadata
- Authors
- Chan, K.-L., Roig, M. B., Hu, B., Beckouët, F., Metson, J., & Nasmyth, K.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2012-08
- Volume
- 150
- Issue
- 5
- Pages
- 961-974
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2003101935
- Cited by
- 253
- References
- 50
- FWCI
- 8.46
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Chan, K.-L., Roig, M. B., Hu, B., Beckouët, F., Metson, J., & Nasmyth, K. (2012). Cohesin’s DNA Exit Gate Is Distinct from Its Entrance Gate and Is Regulated by Acetylation. Cell. https://doi.org/10.1016/j.cell.2012.07.028
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
