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Cited by 214Genomics and Chromatin DynamicsOlfactory and Sensory Function StudiesChromosomal and Genetic Variations

Enhancer Interaction Networks as a Means for Singular Olfactory Receptor Expression

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Markenscoff-Papadimitriou, Eirene, Allen, William E., Colquitt, Bradley M., Goh, Tracie, Murphy, Karl K., Monahan, Kevin, Mosley, Colleen P., Ahituv, Nadav +1 more·2014·Cell
DOI 10.1016/j.cell.2014.09.033PMC PMC4243057
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Abstract

The transcriptional activation of one out of ?2800 olfactory receptor (OR) alleles is a poorly understood process. Here, we identify a plethora of putative OR enhancers and study their in vivo activity in olfactory neurons. Distinguished by an unusual epigenetic signature, candidate OR enhancers are characterized by extensive interchromosomal interactions associated with OR transcription and share a similar pattern of transcription factor footprints. In particular, we establish the role of the transcription factor Bptf as a facilitator of both enhancer interactions and OR transcription. Our observations agree with the model whereby OR transcription occurs in the context of multiple interacting enhancers. Disruption of these interchromosomal interactions results in weak and multigenic OR expression, suggesting that the rare coincidence of numerous enhancers over a stochastically chosen OR may account for the singularity and robustness in OR transcription.

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  • In an enhancer chain, not all enhancers need to contact the gene promoter directly; the signal could be passed along the chain.

    “In this mechanism, not all of the enhancers need to contact the gene promoter directly; rather, the signal could be passed along the chain.”

    From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [488]

    The Results section, “Extensive cis and trans interactions between potential OR enhancers in OSNs” (page unavailable), reports frequent enhancer–enhancer contacts, and the Discussion proposes that multiple enhancers contribute to OR transcription. The paper does not establish or explicitly propose that a signal passes along an enhancer chain so that some enhancers need not contact the promoter directly. (LLM, conf=0.78)

    Experimental — accuracy not yet measured

  • There is evidence for enhancer-enhancer interactions that create an enhancer chain or network.

    “there is also evidence for enhancer–enhancer interactions that create an enhancer “chain” or network [486–489].”

    From Transcriptional enhancers and their communication with gene promoters (2021) · cites this paper as [488]

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Authors
Markenscoff-Papadimitriou, E., Allen, W. E., Colquitt, B. M., Goh, T., Murphy, K. K., Monahan, K., Mosley, C. P., Ahituv, N., & Lomvardas, S.
Affiliations
  • University of California, San Francisco (US)
  • Stanford Medicine (US)
Corresponding author
Lomvardas, Stavros
ORCID
Venue
Cell
Publisher
Elsevier BV
Published
2014-10
Volume
159
Issue
3
Pages
543-557
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2014.09.033
PMC
PMC4243057
OpenAlex
W1970956961
Cited by
214
References
65
Citations by year
2026: 8 · 2025: 18 · 2024: 16 · 2023: 20 · 2022: 14 · 2021: 24 · 2020: 21 · 2019: 26 · 2018: 19 · 2017: 17 · 2016: 14 · 2015: 16 · 2014: 1
FWCI
6.56
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • eScholarship (California Digital Library) (submittedVersion)
Funders
  • National Institutes of Health
  • National Institute on Drug Abuse (R01DA036985, R01DA030320, R01DA036894)
  • National Institute on Deafness and Other Communication Disorders (R01DC013560)
  • National Institute of General Medical Sciences (P30GM138441)
  • National Institute of Diabetes and Digestive and Kidney Diseases (R01DK090382)
  • National Institute of Neurological Disorders and Stroke (R01NS079231)
  • National Center for Research Resources (P41RR019664)
  • Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD059862)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Neuroscience › Sensory Systems › Olfactory and Sensory Function Studies
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
Keywords
olfactory receptor genes, enhancer interactions, interchromosomal interactions, BPTF, transcription factor footprints, epigenetic signatures, olfactory sensory neurons, transcriptional activation
MeSH terms
Bromodomain Containing Proteins, Animals, Enhancer Elements, Genetic, Nerve Tissue Proteins, Nucleoproteins, Transcription Factors, Zebrafish, Transcriptional Activation, Olfactory Receptor Neurons, Receptors, Odorant, Animals, Genetically Modified, Antigens, Nuclear, Mice

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