Non-coding 7S RNA inhibits transcription via mitochondrial RNA polymerase dimerization
Open accessAbstract
The mitochondrial genome encodes 13 components of the oxidative phosphorylation system, and altered mitochondrial transcription drives various human pathologies. A polyadenylated, non-coding RNA molecule known as 7S RNA is transcribed from a region immediately downstream of the light strand promoter in mammalian cells, and its levels change rapidly in response to physiological conditions. Here, we report that 7S RNA has a regulatory function, as it controls levels of mitochondrial transcription both in vitro and in cultured human cells. Using cryo-EM, we show that POLRMT dimerization is induced by interactions with 7S RNA. The resulting POLRMT dimer interface sequesters domains necessary for promoter recognition and unwinding, thereby preventing transcription initiation. We propose that the non-coding 7S RNA molecule is a component of a negative feedback loop that regulates mitochondrial transcription in mammalian cells.
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The non-coding 7S RNA triggers POLRMT dimerization and subsequently regulates mitochondrial transcription.
“A recent study indicates that the non-coding 7S RNA triggers POLRMT dimerization and subsequently regulates mitochondrial transcription through a series of in vitro transcription and transcription pulse-chase experiments. (Zhu et al, 2022).”
From The mitochondrial long non-coding RNA lncMtloop regulates mitochondrial transcription and suppresses Alzheimer’s disease (2024) · cites this paper as Zhu et al, 2022
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Human mitochondrial RNA decay mediated by PNPase–hSuv3 complex takes place in distinct foci
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Making Proteins in the Powerhouse
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“When the non-coding 7S transcript was initially identified in the early 1970s by Giuseppe Attardi and coworkers, they demonstrated that 7S RNA levels were regulated independently of other L-strand transcripts (Aloni and Attardi, 1971; Ojala and Attardi, 1974).”
Background6S RNA: a small RNA regulator of transcription.
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TFB2 is a transient component of the catalytic site of the human mitochondrial RNA polymerase
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Conserved Sequence Box II Directs Transcription Termination and Primer Formation in Mitochondria*
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In vitro-reconstituted nucleoids can block mitochondrial DNA replication and transcription.
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“…to its role in transcription initiation, TFAM is a key factor for packaging mtDNA into nucleoprotein complexes (termed nucleoids) (Alam et al., 2003; Kaufman et al., 2007; Kukat et al., 2011), which directly influences levels of mtDNA replication and gene expression in vitro (Farge et al., 2014).”
BackgroundSelf-citationStructural basis of mitochondrial transcription
Hillen, H. S., Temiakov, D., & Cramer, P. (2018) · Nature Structural & Molecular Biology · doi:10.1038/s41594-018-0122-9 · Cited by 111 · see citing works
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BackgroundTEFM is a potent stimulator of mitochondrial transcription elongation in vitro
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“, 2018), with the loss of mtEXO leading to elevated levels of double-stranded RNA or RNA/DNA hybrids (Dhir et al., 2018; Silva et al., 2018).”
BackgroundA novel intermediate in transcription initiation by human mitochondrial RNA polymerase
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“Received: October 29, 2021 Revised: April 5, 2022 Accepted: May 5, 2022 Published: June 2, 2022 plex or RNase T1 (50 U/mL) rescues the binding of POLRMT and TFB2M at the ntations of the TFAM binding site, POLRMT + TFB2M binding site, and TSS are sing the TSS of LSP.”
InfluentialMitochondrial RNA granules are fluid condensates positioned by membrane dynamics
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“In agreement with this notion, imaging of 7S RNA by FISH revealed evenly distributed, discrete foci (with an average diameter of 140 nm) in cells treated with control siRNA (Figure 3A) (Rey et al., 2020), which in SUV3 depleted cells became 12-fold larger (1.”
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“CSB II pre-termination and run-off transcripts are i tion are dramatically upregulated, but there is a severe depletion of 7S RNA (Jiang et al., 2021).”
Self-citationRNase H1 directs origin-specific initiation of DNA replication in human mitochondria
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“Whereas RNA primers remain hybridized to the DNA template as persistent RNA/DNA hybrids (R-loops) (Posse et al., 2019), 7S RNA is not bound to the DNA template and is instead a polyadenylated single-strand species.”
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“The TFAM-DNA complex recruits POLRMT to the promoter, which positions the active site of the polymerase at the TSS (Morozov et al., 2014; Posse et al., 2014).”
Self-citationStructure of human mitochondrial RNA polymerase elongation complex
Schwinghammer, K., Cheung, A., Morozov, Y. I., Agaronyan, K., Temiakov, D., & Cramer, P. (2013) · Nature Structural &Molecular Biology · doi:10.1038/nsmb.2683 · Cited by 63 · cited 1× · see citing works
“Presumably, the elongating form of the enzyme adopts a conformation, together with TEFM and the DNA template, which prevents 7S RNA interactions and subsequent dimer formation (Hillen et al., 2018; Schwinghammer et al., 2013).”
POLRMT mutations impair mitochondrial transcription causing neurological disease
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Self-citationMitochondrial RNA granules are critically dependent on mtDNA replication factors Twinkle and mtSSB
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“Furthermore, bromouridine (BrU) labeling to visualize de novo transcripts (Hensen et al., 2019) revealed that while newly synthesized RNA appeared as discrete puncta in control cells (Figure 3F), no newly synthesized RNA could be detected from the 7S RNA granules in SUV3 depleted cells (Figures 3F and 3G; Video S1).”
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“After transcription initiation, TFB2M dissociates from the complex, allowing the transcription elongation factor (TEFM) to associate with POLRMT (Hillen et al., 2017a; Posse and Gustafsson, 2017; Posse et al., 2014; Sologub et al., 2009).”
Self-citationOjala, D., Crews, S., Montoya, J., Gelfand, R., & Attardi, G. (1981) · Journal of Molecular Biology · doi:10.1016/0022-2836(81)90454-x · Cited by 53 · cited 4×
“Early reports noted that 7S RNA contains a region complementary to the 30 end of 12S rRNA (Ojala et al., 1981).”
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“, 2018), with the loss of mtEXO leading to elevated levels of double-stranded RNA or RNA/DNA hybrids (Dhir et al., 2018; Silva et al., 2018).”
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“In support of this notion, others have reported that mtEXO activity depends on the length of the poly(A) tail (Razew et al., 2018; Wang et al., 2014).”
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“…appropriate size ( 189 nt) were excised from a SYBR-Gold stained 6% denaturing UreaPAGE gel and recovered by the ‘crush and soak’ method described in (Nilsen, 2013), purifying recovered RNA using a RNA clean and concentrator kit (Zymo), eluting with TE buffer supplemented with 0.16 U/mL RNase…”
InfluentialOjala, D., & Attardi, G. (1974) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.71.2.563 · Cited by 29 · cited 1×
“When the non-coding 7S transcript was initially identified in the early 1970s by Giuseppe Attardi and coworkers, they demonstrated that 7S RNA levels were regulated independently of other L-strand transcripts (Aloni and Attardi, 1971; Ojala and Attardi, 1974).”
Rażew, M., Warkocki, Z., Taube, M., Kolondra, A., Czarnocki-Cieciura, M., Nowak, E., Labedzka-Dmoch, K., Kawińska, A., Piątkowski, J., Golik, P., et al. (2018) · Nature Communications · doi:10.1038/s41467-017-02570-5 · Cited by 26 · cited 1× · see citing works
“In support of this notion, others have reported that mtEXO activity depends on the length of the poly(A) tail (Razew et al., 2018; Wang et al., 2014).”
BackgroundRNase footprinting to map sites of RNA-protein interactions.
Nilsen, T. (2014) · Cold Spring Harbor Protocols · doi:10.1101/pdb.prot080788 · Cited by 23 · cited 1×
“RNase I cleavage reactions were performed based on the protocol described (Nilsen, 2014).”
An evolutionary preserved intergenic spacer in gadiform mitogenomes generates a long noncoding RNA
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Structure of an inactive RNA polymerase II dimer
Aibara, S., Dienemann, C., & Cramer, P. (2021) · Nucleic Acids Research · doi:10.1093/nar/gkab783 · Cited by 16 · cited 1× · see citing works
“It is worth noting that a recent study proposed that the dimerization of RNA Pol II in solutionmay have the potential to inactivate transcription (Aibara et al., 2021).”
RNase H1 Regulates Mitochondrial Transcription and Translation via the Degradation of 7S RNA
Reyes, A., Rusecka, J., Tońska, K., & Zeviani, M. (2020) · Frontiers in Genetics · doi:10.3389/fgene.2019.01393 · Cited by 16 · cited 1×
“For example, in skin fibroblasts from patients with disease-causing RNASEH1 mutations, the levels of 7S RNA are upregulated, whereas other mitochondrial transcripts are depleted (Reyes et al., 2019).”
Noncoding RNAs Set the Stage for RNA Polymerase II Transcription.
Studniarek, C., Egloff, S., & Murphy, S. (2020) · Trends in Genetics · doi:10.1016/j.tig.2020.09.013 · Cited by 10 · cited 1× · see citing works
“Recently, non-coding RNA molecules in the nuclear genome have shown diverse functions such as regulating genome organization and gene expression (Studniarek et al., 2021; Yao et al., 2019).”
Faithful and highly efficient RNA synthesis in isolated mitochondria from rat liver
Cantatore, P., Polosa, P. L., Mustich, A., Petruzzella, V., & Gadaleta, M. (1988) · Current Genetics · doi:10.1007/bf00521272 · Cited by 10 · cited 1×
“7S RNA transcript levels and those of other mitochondrial transcripts, hinting that 7S RNA could act as a negative regulator of transcription, although the underlying mechanisms were unknown (Cantatore et al., 1988).”
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7 of the 70 references couldn’t be matched to a known paper.
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Mechanisms and regulation of human mitochondrial transcription
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Self-citationEmerging functions of mitochondria-encoded noncoding RNAs
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The human mitochondrial mRNA structurome reveals mechanisms of gene expression
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Spaceflight alters host-gut microbiota interactions
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LncRNA–Protein Interactions: A Key to Deciphering LncRNA Mechanisms
Wang, Z., Aftab, M., Dong, Z., Jiang, Y., & Liu, K. (2025) · Biomolecules · doi:10.3390/biom15060881 · Cited by 20 · see citing works
“By using decomposition and negative control methods, researchers were able to map the density of 7S RNA, which appears to bind and regulate the wrapping of POLRMT during dimerization [272].”
Ribonucleotide synthesis by NME6 fuels mitochondrial gene expression
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No role for nuclear transcription regulators in mammalian mitochondria?
Rubalcava-Gracia, D., García-Villegas, R., & Larsson, N. (2022) · Molecular Cell · doi:10.1016/j.molcel.2022.09.010 · Cited by 25 · see citing works
Emerging Mechanisms of Human Mitochondrial Translation Regulation
Brischigliaro, M., Ahn, A., Hong, S.-W., Fontanesi, F., & Barrientos, A. (2025) · TIBS -Trends in Biochemical Sciences. Regular ed · doi:10.1016/j.tibs.2025.03.007 · Cited by 19 · see citing works
Research progresses on mitochondrial-targeted biomaterials for bone defect repair
Wang, S., Liu, J., Zhou, L., Xu, H., Zhang, D., Zhang, X., Wang, Q., & Zhou, Q. H. (2024) · Regenerative Biomaterials · doi:10.1093/rb/rbae082 · Cited by 18 · see citing works
Xiong, W., Xu, K., Sun, J. K.-L., Liu, S., Zhao, B., Shi, J., Herrup, K., Chow, H.-M., Lu, L., & Li, J. (2024) · The EMBO Journal · doi:10.1038/s44318-024-00270-7 · Cited by 17 · see citing works
“(Zhu et al, 2022).”
Mechanisms and pathologies of human mitochondrial DNA replication and deletion formation
Gomes, T. M. B., Vincent, A. E., Menger, K. E., Stewart, J. B., & Nicholls, T. J. J. (2024) · Biochemical Journal · doi:10.1042/bcj20230262 · Cited by 19 · see citing works
Quality control of mitochondrial nucleoids
Liu, H., Zhuang, H., & Feng, D. (2025) · Trends in Cell Biology · doi:10.1016/j.tcb.2025.02.005 · Cited by 20 · see citing works
ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing
Clemente, P., Calvo‐Garrido, J., Pearce, S. F., Schober, F. A., Shigematsu, M., Siira, S. J., Laine, I., Spåhr, H., Steinmetzger, C., Petzold, K., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-33368-9 · Cited by 21 · see citing works
Bi, Y., Yu, L., Deng, Q., Kong, L., Guo, F., Zhang, Y., Wang, R., Chen, P. R., Liu, J., & Fan, X. (2025) · Nature Chemistry · doi:10.1038/s41557-025-01946-1 · Cited by 15 · see citing works
“5 and 6).”
Super-resolution microscopy of mitochondrial mRNAs
Stoldt, S., Maass, F., Weber, M., Dennerlein, S., Ilgen, P., Gärtner, J., Canfes, A., Schweighofer, S. V., Jans, D., Rehling, P., et al. (2025) · bioRxiv · doi:10.1038/s41467-025-61577-5 · Cited by 13 · see citing works
Central dogma rates in human mitochondria.
McShane, E., & Churchman, L. S. (2024) · Human Molecular Genetics · doi:10.1093/hmg/ddae036 · Cited by 11 · see citing works
“Many of these molecules are likely in an inactive state induced by dimerization via the non-coding RNA (7S RNA) [27].”
The human mitochondrial mRNA structurome reveals mechanisms of gene expressionPreprint
Moran, J. C., Brivanlou, A., Brischigliaro, M., Fontanesi, F., Rouskin, S., & Barrientos, A. (2023) · bioRxiv · doi:10.1101/2023.10.31.564750 · Cited by 11 · see citing works
“The L-strand primary transcript contains 1 mRNA that encodes subunit 6 of NADH dehydrogenase (ND6), 8 tRNAs, and non-coding RNAs (ncRNAs) like 7S ncRNA that regulates transcription initiation 11 .”
BackgroundSpatiotemporal Distribution and Function of Mitochondria in Oocytes
Kang, X., Yan, L.-Y., & Wang, J. (2023) · Reproductive Sciences · doi:10.1007/s43032-023-01331-8 · Cited by 10 · see citing works
“Recent studies proposed that a non-coding 7S RNA molecule is part of a negative feedback loop that regulates mitochondrial transcription in mammalian cells [22].”
Li, X., Liu, L., Feng, D., Shi, Y., Huang, L., Yu, M., Fu, X., Deng, L., Nie, X., Chen, C., et al. (2025) · Journal of Medicinal Chemistry · doi:10.1021/acs.jmedchem.5c00130 · Cited by 8 · see citing works
Self-citationYun, J., Shan, J., & Li, J. (2025) · International Urology and Nephrology · doi:10.1007/s11255-025-04651-7 · Cited by 7 · see citing works
“…and transcription, TFAM col-laborates with other transcription factors, such as mitochondrial RNA polymerase (POLRMT), to form transcription initiation complexes that ensure proper mtDNA replication and transcription, thereby supporting the production of essential mitochondrial proteins [59].”
Yan, C.-J., Yu, J., Lyu, H., Xiao, S., Guo, D., Zhang, Q., Zhang, R., Tang, J.-F., Song, Z.-Y., & Zhou, C.-F. (2025) · Biomolecules · doi:10.3390/biom15101379 · Cited by 6
“The 7S RNA is transcribed from the non-coding D-loop region of mitochondrial DNA [52].”
New insights into the relationship of mitochondrial metabolism and atherosclerosis
Wang, Z., Sun, W., Zhang, K., Ke, X., & Wang, Z. (2024) · Cellular Signalling · doi:10.1016/j.cellsig.2024.111580 · Cited by 6 · see citing works
Human mtDNA-Encoded Long ncRNAs: Knotty Molecules and Complex Functions
Bruni, F. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25031502 · Cited by 5 · see citing works
“In turn, this configuration would prevent the enzyme from interacting with the promoter, burying its DNA binding motifs [85].”
BackgroundXu, P., Yang, T., Kundnani, D. L., Sun, M., Marsili, S., Gombolay, A. L., Jeon, Y., Newnam, G., Balachander, S., Bazzani, V., et al. (2023) · Nucleic Acids Research · doi:10.1093/nar/gkad1204 · Cited by 6 · see citing works
“Moreover, some LSP transcripts are prematurely terminated, serve as primers for heavy-strand replication, and form persistent RNA / DNA hybrids ( 50–52 ).”
BackgroundSUV3 Helicase and Mitochondrial Homeostasis
Chen, P.-L. (2023) · International Journal of Molecular Sciences · doi:10.3390/ijms24119233 · Cited by 6 · see citing works
“made an exciting discovery linking mtExo to regulating mitochondrial transcription initiation [77].”
BackgroundHuman mitochondrial RNA polymerase structures reveal transcription start site and slippage mechanism
Shen, J., Goovaerts, Q., Ajjugal, Y., De Wijngaert, B., Das, K., & Patel, S. S. (2025) · Molecular Cell · doi:10.1016/j.molcel.2025.07.002 · Cited by 5 · see citing works
Wang, X., Memon, A., Hedelius, A., Grundberg, A., Sundquist, J., & Sundquist, K. (2023) · Journal of Affective Disorders · doi:10.1016/j.jad.2023.12.053 · Cited by 5
Association of Circulating Long Noncoding 7S RNA with Deep Vein Thrombosis
Wang, X., Memon, A., Hedelius, A., Grundberg, A., Elf, J., Svensson, P., Sundquist, J., & Sundquist, K. (2023) · Seminars in Thrombosis and Hemostasis · doi:10.1055/s-0043-1772705 · Cited by 5
Mitochondrial Transplantation Therapy for Ischemic Stroke: Progress and Challenges
Zhao, Z.-H., Yang, X.-L., Lu, Z., Li, T., Zhao, L.-L., Li, Y., Dang, M., Zhang, G.-L., Chen, K., & Fan, H. (2026) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-026-01682-1 · Cited by 3 · see citing works
“It is well known that mitochondrial DNA (mtDNA) are primarily involved in oxidative phosphorylation (OXPHOS), a crucial process for maintaining cellular energy metabolism (Zhu et al. 2022; Rackham And Filipovska 2022).”
POLRMT overexpression increases mtDNA transcription without affecting steady-state mRNA levels
Miranda, M., Mesaros, A., Scrima, N., Pérard, L., Kuznetsova, I., Purrio, M., Atanassov, I., Filipovska, A., Mourier, A., Larsson, N., et al. (2025) · Life Science Alliance · doi:10.26508/lsa.202302563 · Cited by 4 · see citing works
Wang, R., Chen, B., Pan, Y., Wang, M., Xiao, Y., Shi, D., Wen, Y.-L., Ouyang, Y., Chen, X.-F., Li, Y., et al. (2025) · Life Science · doi:10.1016/j.lfs.2025.123876 · Cited by 4 · see citing works
Transcription arrest induces formation of RNA granules in mitochondria
Hansen, K. G., Baxter-Koenigs, A. R., Weiss, C. A. M., McShane, E., & Churchman, L. S. (2025) · Life Science Alliance · doi:10.26508/lsa.202403082 · Cited by 4
“Rather, we introduce SUV3 now as a condition where we observe RNA granules similar to the EtBr and IMT1B treatments, consistent with its known role in transcription regulation: “Recently, it has been found that downregulation of SUV3 leads to reduced transcription (Zhu et al. 2022).”
Tahira, A. C., Gomes, M. P. B., Freire, M. H. O., Muxfeldt, M., Prosdocimi, F., Passos, Y. M., Amaral, M. S., Valadão, L. P. F., Rangel, L. P., Silva, J. L., et al. (2025) · RNA Biology · doi:10.1080/15476286.2025.2508107 · Cited by 5 · see citing works
“7S RNAs are abundant non-coding cytosolic/ mitochondrial polyadenylated RNAs predicted to regulate transcription initiation in mammalian mitochondria [60].”
Matsuda, S., Nakayama, M., Do, Y., Ishiuchi, T., Yagi, M., Wanrooij, S., Nakada, K., Wei, F.-Y., Ichiyanagi, K., Sasaki, H., et al. (2025) · Communications Biology · doi:10.1038/s42003-025-07645-4 · Cited by 4 · see citing works
The initiation of mitochondrial DNA replication
Liu, Y., Liu, H., Zhang, F., & Xu, H. (2024) · Biochemical Society Transactions · doi:10.1042/bst20230952 · Cited by 4 · see citing works
Bacterial small regulatory RNAs
Papenfort, K. (2026) · Nature Reviews Microbiology · doi:10.1038/s41579-026-01353-4 · Cited by 4 · see citing works
Loop-mediated regulation and base flipping drive RNA cleavage by human mitochondrial PNPase
Unseld, O., Das, H., & Hällberg, B. M. (2025) · Nucleic Acids Research · doi:10.1093/nar/gkaf1296 · Cited by 3 · see citing works
“…(hPNPase) is localized to the mitochondria, where it plays critical roles in maintaining RNA homeostasis through the degradation of mitochondrial RNA antisense transcripts, R-loop processing, and transcription inhibition relief together with the human mitochondrial helicase hSuv3 [ 5–9 ].”
Self-citationSomai, S., Aloh, C. H., King, D. E., & Copeland, W. C. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms262110275 · Cited by 3 · see citing works
“Although the exact function of the D-loop is currently unknown, several studies have proposed that the D-loop might play a role in nucleoid organization and may be involved in the regulation of mtDNA replication [18].”
Decoding Mitochondrial–Nuclear (epi)genome Interactions: The Emerging Role of ncRNAs
Nguyen, J., Le, Q., Win, P. W., Hill, K. A., Singh, S. M., & Castellani, C. A. (2023) · Epigenomics · doi:10.2217/epi-2023-0322 · Cited by 3 · see citing works
Mammalian mtDNA gene expression: Concepts learned from in vivo models.
Rubalcava-Gracia, D., & Larsson, N. (2026) · Biochimica et biophysica acta. Molecular cell research · doi:10.1016/j.bbamcr.2026.120158 · Cited by 2 · see citing works
Asymmetric dimeric assembly of Suv3 helicase facilitates processive RNA unwinding
Patra, M., Jain, M., Li, Y., Chen, Y.-P., Golzarroshan, B., & Yuan, H. S. (2026) · Nature Communications · doi:10.1038/s41467-026-71901-2 · Cited by 2 · see citing works
“This dimeric organization is also functional essential, as key activities, such asG-quadruplex RNA resolution and 7S RNA targeting, strictly depend on Suv3 dimerization 14,65 .”
Tiny genome with big impact: mitochondrial DNA in cardiovascular health.
Yang, Y., Li, J., Qian, L., Xiong, Y.-Y., & Yu, Y. (2025) · Journal of genetics and genomics = Yi chuan xue bao · doi:10.1016/j.jgg.2025.12.009 · Cited by 2 · see citing works
Cellular metabolic state controls mitochondrial RNA kineticsPreprint
Reardon, S. D., Bautista, C., Cole, S., Cicero, J., & Mishanina, T. V. (2025) · bioRxiv · doi:10.1101/2025.05.13.653903 · Cited by 2 · see citing works
“A recently characterized mitochondrial non-coding RNA, the 7S RNA, has been shown to directly inhibit POLRMT in cells and in vitro 27 .”
ncRNA regulation of mitochondrial transcription
Zlotorynski, E. (2022) · Nature reviews. Molecular cell biology · doi:10.1038/s41580-022-00509-3 · Cited by 2
Mitochondrial RNA polyadenylation: Defining RNA fate through 3' end regulation.
Muellner-Wong, L. S., Carter, W., Freyer, C., & Wredenberg, A. (2026) · Biochimica et biophysica acta. Molecular cell research · doi:10.1016/j.bbamcr.2026.120207 · Cited by 1 · see citing works
Tang, G.-X., Yan, J.-T., Li, M.-L., Zhou, C., Wang, J., Chen, S.-B., Huang, Z.-S., & Tan, J.-H. (2026) · Advancement of science · doi:10.1002/advs.202523462 · Cited by 1
“In addition, mtDNA encodes various noncoding RNAs (e.g., 7S RNA and lncND5) [ 2, 3 ].”
The Role of Long Non-Coding RNA in Anxiety Disorders: A Literature Review
López-Rocha, L. D., Ruíz-Hernández, A., Martínez-Coronilla, G., Leija-Montoya, A. G., Peña-Peña, M., Sánchez‐Muñoz, F., Campoy, U. R., & González-Ramírez, J. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms26115042 · Cited by 3 · see citing works
“The mitochondrial lncRNA 7S is known as a gene regulator capable of controlling mitochondrial transcription [70].”
A phenotypic drug discovery approach by latent interaction in deep learning
Yu, T.-W. (2024) · Royal Society Open Science · doi:10.1098/rsos.240720 · Cited by 1 · see citing works
Research progress and prospect in mitochondrial-targeted therapy
Wang, T.-Y., Wang, Y.-H., Koivisto, O., Cui, Y.-B., & Zhang, H.-B. (2026) · Biomedical Technology · doi:10.1016/j.bmt.2026.100183
Sharkey, R. E., Schroeder, C. M., Deng, X.-Y., Smith, J., Hernandez, A. J., & Gao, Y. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.113611
“While PolRMT dimerization via 7S RNA inhibits transcription (34), a different interface was found for dimerization of the apo protein (23), and it is unknown if a higher-order organization of both promoters may involve transcriptionally productive PolRMT dimerization.”
InfluentialZhan, Y., Liang, G.-Y., Wang, J., Cen, X.-F., Zhang, N., Zhao, N., Xing, Y., Li, L.-L., Yu, T., Dong, W.-S., et al. (2026) · Research · doi:10.34133/research.1400
“…epi-transcrip-tome has accelerated the exploration of mtRNA modification [ 11 ] and linked mtRNA modification to metabolic plasticity [ 12 , 13 ], the precise molecular mechanisms underlying the involvement of nuclear genome-derived RNA (encoding mitochondrial protein) metabolism in…”
Pathogenic POLRMT variants in mice impair mtDNA transcription and affect perinatal survival
Alsina, D., Rubalcava-Gracia, D., Bubb, K., García-Villegas, R., Végvári, Á., Filograna, R., Rosenberger, F., Koolmeister, C., & Larsson, N.-G. (2026) · JCI Insight · doi:10.1172/jci.insight.199182
“To activate POLRMT, the mtEXO complex degrades the 7S RNA, thereby relieving the inhibition and allowing transcription to resume (15).”
MTERF1 loss buffers against pathogenic mtDNA deletions through transcriptional regulation
Kavlashvili, T., Choi, E., Cui, R.-B., Landoni, J., Zhang, S., Isaac, S. R., Churchman, S., Manley, S., Thompson, C. B., & Sfeir, A. (2026) · bioRxiv · doi:10.64898/2026.07.16.738964
Higher-order structural organization of mitochondrial metabolism
Kafetzopoulos, G., Döbler, J., & Kastritis, P. L. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.113174
“Specific mutations in the polymerase abolish dimerization (245).”
Shakey, R. E., Schroeder, C. M., Deng, X.-Y., Smith, J., Hernandez, A. J., & Gao, Y. (2026) · bioRxiv · doi:10.64898/2026.04.09.717523
“Furthermore, the non-coding 7S RNA was recently shown to stimulate formation of a transcriptionally-inactive PolRMT dimer (22).”
RNA-STR/SNP: A mirror of forensic DNA-STR/SNP.
Liu, Z.-Y., Liu, J.-J., Wu, R., Zhao, K., Yang, X., Xie, J.-M., Wang, R., Wu, E.-L., Chen, W.-H., Cong, B., et al. (2026) · Forensic Science International: Genetics · doi:10.1016/j.fsigen.2026.103489
Subcellular Stress Markers in Epithelial Ovarian Cancer
Wappler-Guzzetta, E. A., Margittai, É., Veszelyi, K., Pickard, S. C., Merwin, C., Molvarec, A., & Czegle, I. (2025) · International Journal of Molecular Sciences · doi:10.3390/ijms27010342
“On the other hand, transcription inhibition was described with 7S RNA, which specifically inhibits POLRMT activity [36].”
Identification of determinants for variability in mitochondrial biochemical complex activities
de Courtade, S. M. B., Eikenes, M., Sheng, Y., Nyman, T. A., Bliksrud, Y. T., Scheffler, K., & Eide, L. (2025) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2025.149553
- Authors
- Zhu, X., Xie, X., Das, H., Tan, B. G., Shi, Y., Al-Behadili, A., Peter, B., Motori, E., Valenzuela, S., Posse, V., Gustafsson, C. M., Hällberg, B. M., & Falkenberg, M.
- Affiliations
- University of Gothenburg (SE)
- Karolinska Institutet (SE)
- University of Cologne (DE)
- Corresponding author
- Hällberg, B. Martin
- Falkenberg, Maria
- ORCID
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 2022-06
- Volume
- 185
- Issue
- 13
- Pages
- 2309-2323.e24
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W4281995370
- Cited by
- 69
- References
- 70
- Citations by year
- 2026: 14 · 2025: 20 · 2024: 19 · 2023: 11 · 2022: 5
- FWCI
- 4.49
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Cell (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Kölner Universitäts PublikationsServer (Universität zu Köln) (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › RNA and protein synthesis mechanisms
- Keywords
- 7S RNA, mitochondrial RNA polymerase, RNA-protein interactions, transcriptional regulation, mitochondrial transcription, non-coding RNA, negative feedback loop, transcription initiation, oxidative phosphorylation
- MeSH terms
- RNA, Mitochondrial, Animals, DNA, Mitochondrial, Humans, Mammals, RNA, DNA-Directed RNA Polymerases, Transcription, Genetic, Signal Recognition Particle, Dimerization, RNA, Small Cytoplasmic, Mitochondrial Proteins
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