A new PKCα/β/TBX3/E-cadherin pathway is involved in PLCε-regulated invasion and migration in human bladder cancer cells
Abstract
Although PLCε has been verified to enhance bladder cancer cell invasion, the signaling pathways responsible for this remain elusive. Protein kinase C (PKCα/β), which is involved in cancer development and progression, has been demonstrated to be activated by PLCε. However, the roles of PKCα/β in PLCε-mediated bladder carcinoma cell invasion and migration have not been clearly identified. In this study, to determine what role PKCα/β plays in PLCε-mediated bladder cancer cell invasion and migration, we silenced PLCε gene by adenovirus-shPLCε in T24 and BIU-87 cells and then revealed that it significantly inhibited cell migration and invasion. Further research indicated that cell bio-function of PLCε-regulated was related with PKCα/β activity. These in vitro findings were supported by data from bladder carcinoma patient samples. In 35 case bladder cancer tumor samples, PLCε-overexpressing tumors showed significantly higher positive rates of PKCα/β membrane immunohistochemistry staining than PLCε-low-expressing tumors. Mechanistically, study further showed that PLCε knockdown gene induced E-cadherin expression and decreased TBX3 expression, both of which were dependent on PKCα/β activity. In addition, we demonstrated that treatment cells with TBX3-specific shorting hairpin RNA (shRNA) up-regulated E-cadherin expression and inhibited cell invasion/migration. Moreover, in in vivo experiment, immunohistochemistry analysis of Ad-shPLCε-infected tumor tissue showed low expression level…
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Although there are some studies indicating TBX3 is an oncogene in BLCA, the role of TBX3 in the TME and tumor progression is not fully understood.
“Although there are some studies indicating TBX3 is an oncogene in BLCA 31,32, the role of TBX3 in the TME and tumor progression is not fully understood.”
From TBX3 shapes an immunosuppressive microenvironment and induces immunotherapy resistance (2025) · cites this paper as [31]
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References 39 · Cited by 43
- Authors
- Du, H. F., Ou, L. P., Yang, X., Song, X. D., Fan, Y. R., Tan, B., Luo, C. L., & Wu, X. H.
- Affiliations
- Chongqing Medical University (CN)
- The Affiliated Yongchuan Hospital of Chongqing Medical University (CN)
- Corresponding author
- Luo, Chun Li
- Venue
- Cellular Signalling
- Publisher
- Elsevier BV
- Published
- 2014-03
- Volume
- 26
- Issue
- 3
- Pages
- 580-593
- ISSN
- 0898-6568 (ISSN-L)
- OpenAlex
- W2082355469
- Cited by
- 43
- References
- 39
- Citations by year
- 2026: 1 · 2024: 2 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 6 · 2019: 4 · 2018: 3 · 2017: 6 · 2016: 7 · 2015: 7 · 2014: 2
- FWCI
- 5.25
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cellular Signalling (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of P.R. China (81072086)
- Scientific and Technological Research Program of Chongqing Municipal Education Committee of the People's Republic of China (KJ110305)
- Topics
- Health Sciences › Medicine › Surgery › Bladder and Urothelial Cancer Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Keywords
- phospholipase C epsilon, TBX3, E-cadherin, bladder cancer, cell invasion, cell migration, T24 cells, BIU-87 cells
- MeSH terms
- Animals, Carbazoles, Cell Movement, Humans, Mice, Nude, Neoplasm Invasiveness, Neoplasm Transplantation, Transplantation, Heterologous, Cadherins, T-Box Domain Proteins, RNA Interference, RNA, Small Interfering, Cell Line, Tumor, Cell Proliferation, Mice, Protein Kinase C-alpha, Phosphoinositide Phospholipase C, Protein Kinase C beta
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