Bicaudal d family adaptor proteins control the velocity of Dynein-based movements
Open accessAbstract
Cargo transport along microtubules is driven by the collective function of microtubule plus- and minus-end-directed motors (kinesins and dyneins). How the velocity of cargo transport is driven by opposing teams of motors is still poorly understood. Here, we combined inducible recruitment of motors and adaptors to Rab6 secretory vesicles with detailed tracking of vesicle movements to investigate how changes in the transport machinery affect vesicle motility. We find that the velocities of kinesin-based vesicle movements are slower and more homogeneous than those of dynein-based movements. We also find that Bicaudal D (BICD) adaptor proteins can regulate dynein-based vesicle motility. BICD-related protein 1 (BICDR-1) accelerates minus-end-directed vesicle movements and affects Rab6 vesicle distribution. These changes are accompanied by reduced axonal outgrowth in neurons, supporting their physiological importance. Our study suggests that adaptor proteins can modulate the velocity of dynein-based motility and thereby control the distribution of transport carriers.
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The presence of the N-terminal FKBP domain in the Rab6a construct has no detectable effects on the behavior of this marker.
“The presence of the N-terminal FKBP domain in the Rab6a construct has no detectable effects on the behavior of this marker (Schlager et al., 2014; Serra-Marques et al., 2020).”
From A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle (2023) · cites this paper as Schlager et al., 2014
Figure 1 and the section on kinesin-driven Rab6 movement show that the authors used and imaged FKBP-tagged Rab6 vesicles. Our copy does not report a comparison with Rab6 lacking FKBP or state that the N-terminal FKBP domain has no detectable effect on marker behavior (searched for FKBP-tag effects and tagged-versus-untagged controls). (LLM, conf=0.72)
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Research works printed but not linked: 3
- Allan, V.J. (2011). Cytoplasmic dynein. Biochem. Soc. Trans. 39, 1169-1178.
- Jolly, A.L., and Gelfand, V.I. (2011). Bidirectional intracellular transport: utility and mechanism. Biochem. Soc. Trans. 39, 1126-1130.
- Lipka, J., Kuijpers, M., Jaworski, J., and Hoogenraad, C.C. (2013). Mutations in cytoplasmic dynein and its regulators cause malformations of cortical devel- opment and neurodegenerative diseases. Biochem. Soc. Trans. 41, 1605- 1612.
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Self-propelling vesicles define glycolysis as the minimal energy machinery for neuronal transport
Hinckelmann, M.-V., Virlogeux, A., Niehage, C., Poujol, C., Choquet, D., Hoflack, B., Zala, D., & Saudou, F. (2016) · Nature Communications · doi:10.1038/ncomms13233 · Cited by 103 · see citing works
The Rab6-regulated KIF1C kinesin motor domain contributes to Golgi organization
Lee, P. L., Ohlson, M. B., & Pfeffer, S. R. (2015) · eLife · doi:10.7554/elife.06029 · Cited by 65 · see citing works
A live-cell marker to visualize the dynamics of stable microtubules throughout the cell cycle
Jansen, K. I., Iwanski, M. K., Burute, M., & Kapitein, L. C. (2023) · Journal of Cell Biology · doi:10.1083/jcb.202106105 · Cited by 39 · see citing works
- Authors
- Schlager, M. A., Serra-Marques, A., Grigoriev, I., Gumy, L. F., Esteves da Silva, M., Wulf, P. S., Akhmanova, A., & Hoogenraad, C. C.
- Affiliations
- Erasmus MC (NL)
- Utrecht University (NL)
- Corresponding author
- Akhmanova, Anna
- Hoogenraad, Casper C.
- ORCID
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2014-09
- Volume
- 8
- Issue
- 5
- Pages
- 1248-1256
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2017440930
- Cited by
- 115
- References
- 0
- Citations by year
- 2026: 5 · 2025: 5 · 2024: 10 · 2023: 8 · 2022: 12 · 2021: 9 · 2020: 11 · 2019: 14 · 2018: 10 · 2017: 12 · 2016: 9 · 2015: 9 · 2014: 1
- FWCI
- 4.77
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Utrecht University Repository (Utrecht University) (submittedVersion, other-oa)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and Kidney Cyst Diseases
- Keywords
- Bicaudal-D, dynein, kinesin, vesicle trafficking, adaptor proteins, axon growth, neuronal transport
- MeSH terms
- Animals, Axonal Transport, Cells, Cultured, Dyneins, HeLa Cells, Humans, Microtubule-Associated Proteins, Neurons, Protein Binding, Kinesins, Protein Transport, Adaptor Proteins, Vesicular Transport, Rats
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