The DOI matches its Crossref record; 42 of the 42 references it cites were found in a registry or an index.
Genome Engineering with CRISPR-Cas9 in the Mosquito Aedes aegypti
Open accessAbstract
The mosquito Aedes aegypti is a potent vector of the chikungunya, yellow fever, and dengue viruses, responsible for hundreds of millions of infections and over 50,000 human deaths per year. Mutagenesis in Ae. aegypti has been established with TALENs, ZFNs, and homing endonucleases, which require the engineering of DNA-binding protein domains to provide genomic target sequence specificity. Here, we describe the use of the CRISPR-Cas9 system to generate site-specific mutations in Ae. aegypti. This system relies on RNA-DNA base-pairing to generate targeting specificity, resulting in efficient and flexible genome-editing reagents. We investigate the efficiency of injection mix compositions, demonstrate the ability of CRISPR-Cas9 to generate different types of mutations via disparate repair mechanisms, and report stable germline mutations in several genomic loci. This work offers a detailed exploration into the use of CRISPR-Cas9 in Ae. aegypti that should be applicable to non-model organisms previously out of reach of genetic modification.
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Cites
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The new frontier of genome engineering with CRISPR-Cas9
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Cited by
Bidaye, S. S., Bockemühl, T., & Büschges, A. (2018) · Journal of Neurophysiology · doi:10.1152/jn.00658.2017 · Cited by 152
Insert, remove or replace: A highly advanced genome editing system using CRISPR/Cas9
Ceasar, S. A., Rajan, V., Prykhozhij, S. V., Berman, J. N., & Ignacimuthu, S. (2016) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2016.06.009 · Cited by 151
(2023) · doi:10.7554/elife.80489 · Cited by 34
Metadata
- Authors
- Kistler, K. E., Vosshall, L. B., & Matthews, B. J.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2015-04
- Volume
- 11
- Issue
- 1
- Pages
- 51-60
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2155775409
- Cited by
- 403
- References
- 42
- FWCI
- 15.44
- Type
- journal article
- Language
- en
- Open access
- gold
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