USP7 is a suppressor of PCNA ubiquitination and oxidative-stress-induced mutagenesis in human cells.
Open accessAbstract
Mono-ubiquitinated PCNA activates error-prone DNA polymerases; therefore, strict regulation of PCNA mono-ubiquitination is crucial in avoiding undesired mutagenesis. In this study, we used an in vitro assay system to identify USP7 as a deubiquitinating enzyme of mono-ubiquitinated PCNA. Suppression of USP1, a previously identified PCNA deubiquitinase, or USP7 increased UV- and H2O2-induced PCNA mono-ubiquitination in a distinct and additive manner, suggesting that USP1 and USP7 make different contributions to PCNA deubiquitination in human cells. Cell-cycle-synchronization analyses revealed that USP7 suppression increased H2O2-induced PCNA ubiquitination throughout interphase, whereas USP1 suppression specifically increased ubiquitination in S-phase cells. UV-induced mutagenesis was elevated in USP1-suppressed cells, whereas H2O2-induced mutagenesis was elevated in USP7-suppressed cells. These results suggest that USP1 suppresses UV-induced mutations produced in a manner involving DNA replication, whereas USP7 suppresses H2O2-induced mutagenesis involving cell-cycle-independent processes such as DNA repair.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
This defect seems due to a role of USP7 in regulating ubiquitination of the PCNA sliding clamp.
“This defect seems due to a role of USP7 in regulating ubiquitination of the PCNA sliding clamp (Kashiwaba et al., 2015; Qian et al., 2015; Masuda et al., 2019).”
From USP7 Is a Master Regulator of Genome Stability (2020) · cites this paper as Kashiwaba et al., 2015
USP7 deubiquitinates PCNA during interphase to suppress PCNA monoubiquitination in response to oxidative and, to a lesser extent, UV-induced DNA damage.
“Most directly, USP7 has been reported to deubiquitinate PCNA during interphase of the cell cycle, to suppress PCNA monoubiquitination in response to oxidative – and to a lesser extent, UV-induced – DNA damage (Kashiwaba et al., 2015).”
From USP7 Is a Master Regulator of Genome Stability (2020) · cites this paper as Kashiwaba et al., 2015
The citation lists haven’t been fetched yet
References 39 · Cited by 71
- Authors
- Kashiwaba, S.-I., Kanao, R., Masuda, Y., Kusumoto-Matsuo, R., Hanaoka, F., & Masutani, C.
- Affiliations
- Research Institute of Environmental Medicine, Nagoya University (JP)
- Gakushuin University (JP)
- Corresponding author
- Masutani, Chikahide
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2015-12
- Volume
- 13
- Issue
- 10
- Pages
- 2072-2080
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2195280029
- Cited by
- 71
- References
- 39
- Citations by year
- 2026: 4 · 2025: 6 · 2024: 6 · 2023: 5 · 2022: 7 · 2021: 6 · 2020: 12 · 2019: 8 · 2018: 2 · 2017: 9 · 2016: 6
- FWCI
- 2.46
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Japan Society for the Promotion of Science (25241011, 15K14953, 26740017)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ubiquitin and proteasome pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
- Keywords
- USP7, USP1, PCNA, monoubiquitination, deubiquitinase, oxidative stress, UV mutagenesis, DNA replication, DNA repair, error-prone DNA polymerases, S phase, cell cycle
- MeSH terms
- Ubiquitin-Specific Peptidase 7, Cell Line, DNA Damage, Flow Cytometry, Humans, Hydrogen Peroxide, Transfection, Polymerase Chain Reaction, Mutagenesis, Mutagenesis, Site-Directed, Oxidative Stress, Proliferating Cell Nuclear Antigen, RNA, Small Interfering, Ubiquitin Thiolesterase, Ubiquitination
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
