In Vivo HIV-1 Cell-to-Cell Transmission Promotes Multicopy Micro-compartmentalized Infection
Open accessAbstract
HIV-1 infection is enhanced by adhesive structures that form between infected and uninfected T cells called virological synapses (VSs). This mode of transmission results in the frequent co-transmission of multiple copies of HIV-1 across the VS, which can reduce sensitivity to antiretroviral drugs. Studying HIV-1 infection of humanized mice, we measured the frequency of co-transmission and the spatiotemporal organization of infected cells as indicators of cell-to-cell transmission in vivo. When inoculating mice with cells co-infected with two viral genotypes, we observed high levels of co-transmission to target cells. Additionally, micro-anatomical clustering of viral genotypes within lymphoid tissue indicates that viral spread is driven by local processes and not a diffuse viral cloud. Intravital splenic imaging reveals that anchored HIV-infected cells induce arrest of interacting, uninfected CD4(+) T cells to form Env-dependent cell-cell conjugates. These findings suggest that HIV-1 spread between immune cells can be anatomically localized into infectious clusters.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Cell-to-cell infection enhances co-transmission of two different strains of HIV-1 in vitro and in vivo; cell-cell contact can increase multiplicity of infection and diversity of HIV.
“Law et al. have shown that cell-to-cell infection enhances co-transmission of two different strains of HIV-1 in vitro and in vivo [129]. This is well consistent with our results obtained using cell lines and two reporter vectors, inGFPt and in-mCherry [131], suggesting that cell-cell contact can increase multiplicity of infection and diversity of HIV.”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [129]
HIV-infected T cells in humanized mice circulate and migrate to lymph nodes and spleen, interact with uninfected cells, arrest motility to establish contact-dependent transfer, then move apart forming long tethering membrane protrusions.
“Studies in humanized mice demonstrate that HIV-infected T cells retain the ability to circulate and migrate to the lymph nodes and spleen, where they can interact with uninfected cells, arrest their motility to establish contact-dependent transfer of HIV, and then move apart from each other with the formation of long tethering membrane protrusions [109,129].”
From HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread (2022) · cites this paper as [129]
Cites
Rudnicka, D., Feldmann, J., Porrot, F., Wietgrefe, S., Guadagnini, S., Prévost, M.-C., Estaquier, J., Haase, A. T., Sol-Foulon, N., & Schwartz, O. (2009) · Journal of Virology · doi:10.1128/jvi.00282-09 · Cited by 229 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
HIV-1 and HTLV-1 Transmission Modes: Mechanisms and Importance for Virus Spread
Kalinichenko, S., Komkov, D., & Mazurov, D. (2022) · Viruses · doi:10.3390/v14010152 · Cited by 68 · see citing works
“Studies in humanized mice demonstrate that HIV-infected T cells retain the ability to circulate and migrate to the lymph nodes and spleen, where they can interact with uninfected cells, arrest their motility to establish contact-dependent transfer of HIV, and then move apart from each other with the formation of long…”
Background
- Authors
- Law, K. M., Komarova, N. L., Yewdall, A. W., Lee, R. K., Herrera, O. L., Wodarz, D., & Chen, B. K.
- Affiliations
- Icahn School of Medicine at Mount Sinai (US)
- University of California, Irvine (US)
- Mount Sinai Hospital (US)
- Corresponding author
- Chen, Benjamin K.
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2016-06
- Volume
- 15
- Issue
- 12
- Pages
- 2771-2783
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2412450517
- Cited by
- 101
- References
- 1
- Citations by year
- 2026: 5 · 2025: 7 · 2024: 4 · 2023: 6 · 2022: 10 · 2021: 12 · 2020: 14 · 2019: 14 · 2018: 19 · 2017: 7 · 2016: 3
- FWCI
- 9.93
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institutes of Health
- National Institute on Drug Abuse (F31DA036425)
- National Institute of General Medical Sciences
- National Institute of Allergy and Infectious Diseases (R01AI093998, R01AI074420)
- Topics
- Life Sciences › Immunology and Microbiology › Virology › HIV Research and Treatment
- Health Sciences › Medicine › Infectious Diseases › HIV/AIDS Research and Interventions
- Life Sciences › Immunology and Microbiology › Immunology › T-cell and Retrovirus Studies
- Keywords
- HIV-1, cell-to-cell transmission, virological synapse, co-transmission, humanized mice, viral genotypes, CD4+ T cells, intravital imaging, lymphoid tissue, antiretroviral resistance, viral spread
- MeSH terms
- Animals, Cell Movement, Cell-Free System, Cells, Cultured, Humans, Spleen, CD4-Positive T-Lymphocytes, HIV-1, HIV Infections, Tropism, Gene Dosage, Receptors, CCR5, Mice, env Gene Products, Human Immunodeficiency Virus, Immunological Synapses
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
