The NLR Protein NLRP6 Does Not Impact Gut Microbiota Composition
Open accessAbstract
NLRP6 is a Nod-like receptor expressed in the intestinal epithelium. Previous studies reported a protective role for NLRP6 against intestinal injury and colitis-associated carcinogenesis via the regulation and establishment of a healthy microbiota. However, these results were not obtained using littermate animals, leaving the possibility that the pro-colitogenic microbiota phenotype associated with knockout (KO) mice was stochastically acquired and genotype independent. Here, we analyzed the microbiota at three intestinal locations from Nlrp6 −/− and wild-type (WT) littermates, either co-caged or individually caged after weaning. Our results demonstrate that NLRP6 does not significantly influence the intestinal microbiota at homeostasis, and they support a previously reported sex-biased microbial community structure. Moreover, WT and Nlrp6 −/− littermate mice displayed comparable sensitivity to dextran sulfate sodium (DSS)-induced colitis, although increased sensitivity was noted in KO females. Our results clarify the role of NLRP6 in microbiota and colitis control, and they highlight the importance of analyzing littermate animals in such studies.
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In different facilities, mice lacking ASC, NLRP6, or NLRP3 fail to show any detectable differences in the composition of their fecal flora compared to wild-type controlled littermates, either co-housed or individually housed, contrary to what has been described in other facilities.
“In fact, in different facilities, mice lacking ASC, NLRP6 (199), or NLRP3 (200) fail to show any detectable differences in the composition of their fecal flora compared to wild-type controlled littermates (199), either co-housed or individually housed, contrary to what has been described in other facilities (194).”
From The gut-brain axis: how microbiota and host inflammasome influence brain physiology and pathology (2020) · cites this paper as [200]
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References 22 · Cited by 98
- Authors
- Lemire, P., Robertson, S. J., Maughan, H., Tattoli, I., Streutker, C. J., Platnich, J. M., Muruve, D. A., Philpott, D. J., & Girardin, S. E.
- Affiliations
- University of Toronto (CA)
- Ronin Institute (US)
- St. Michael's Hospital (CA)
- University of Calgary (CA)
- Corresponding author
- Robertson, Susan J.
- ORCID
- Venue
- Cell Reports
- Publisher
- Elsevier BV
- Published
- 2017-12
- Volume
- 21
- Issue
- 13
- Pages
- 3653-3661
- ISSN
- 2211-1247 (ISSN-L)
- OpenAlex
- W2781320385
- Cited by
- 98
- References
- 22
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 9 · 2023: 13 · 2022: 10 · 2021: 10 · 2020: 16 · 2019: 23 · 2018: 10
- FWCI
- 5.47
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Licence
- Locations
- Cell Reports (publishedVersion, cc-by-nc-nd)
- PubMed (publishedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- Canadian Institutes for Health Research (THC-13523)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Inflammatory Bowel Disease
- Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
- Keywords
- NLRP6, NOD-like receptors, gut microbiota, intestinal epithelium, colitis, DSS-induced colitis, sex differences, knockout mice, microbiota composition, colitis-associated carcinogenesis
- MeSH terms
- Gastrointestinal Microbiome, Animals, Colitis, Female, Genotype, Male, Mice, Inbred C57BL, Receptors, Cell Surface, Dextran Sulfate, Mice, Knockout, Microbiota
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