Single-Nucleus RNA-Seq Is Not Suitable for Detection of Microglial Activation Genes in Humans
Thrupp, Nicola, Sala Frigerio, Carlo, Wolfs, Leen, Skene, Nathan G., Fattorelli, Nicola, Poovathingal, Suresh, Fourne, Yannick, Matthews, Paul M. +4 more·2020·Cell Reports
Cited by (344)
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- Transcriptomic Signatures of Hippocampal Subregions and Neuronal Nuclei in Active Place Avoidance Memory MaintenanceIsaac Vingan, Shwetha Phatarpekar, Victoria Sook Keng Tung, Alejandro Ivan Hernandez, Oleg Vadimovich Evgrafov, Juan Marcos Alarcón · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
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- Voluntary Long‐Term Exercise by Female Mice Modulates Anxiety‐Like Behavior and Motor Function but Minimally Impacts Acute Oxidative Injury in the Central Nervous SystemKhanh Ta, Ruoqi Yu, Marzieh Sedighipour Chafjiri, KimAnh Trang Pioli, Aiden E. Glass, Mitchell P. Anstey, Rachel A. Dignean, Justin J. Botterill, Peter D. Pioli, John G. Howland et al. · 2025 · European Journal of Neuroscience
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- Additional file 2 of Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer’s diseaseChen, Shuo, Chang, Yuzhou, Liangping Li, Diana M. Acosta, Li, Yang, Qi Guo, Wang, Cankun, Emir Turkes, Morrison, Cody, Dominic L. Julian et al. · 2023 · Figshare
- Additional file 4 of Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS functionLuise A. Seeker, Nadine Bestard-Cuche, Sarah Jäkel, Kazakou, Nina-Lydia, Sunniva M. K. Bøstrand, Laura J. Wagstaff, Cholewa-Waclaw, Justyna, Alastair Morris Kilpatrick, David van Bruggen, Mukund Kabbe et al. · 2024 · Figshare
- Additional file 1 of Dysregulation of neuroprotective astrocytes, a spectrum of microglial activation states, and altered hippocampal neurogenesis are revealed by single-cell RNA sequencing in prion diseaseJessy A. Slota, Sajesh, Babu V., Kathy F. Frost, Sarah J. Medina, Stephanie A. Booth · 2022 · Figshare
- Additional file 1 of Ambient RNAs removal of cortex-specific snRNA-seq reveals Apoe+ microglia/macrophage after deeper cerebral hypoperfusion in miceZhang, Yuan, Tan, Jinyun, Yang, Kai, Fan, Weijian, Yu, Bo, Shi, Weihao · 2023 · Figshare
- Additional file 2 of Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS functionLuise A. Seeker, Nadine Bestard-Cuche, Sarah Jäkel, Kazakou, Nina-Lydia, Sunniva M. K. Bøstrand, Laura J. Wagstaff, Cholewa-Waclaw, Justyna, Alastair Morris Kilpatrick, David van Bruggen, Mukund Kabbe et al. · 2024 · Figshare
- Additional file 1 of Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS functionLuise A. Seeker, Nadine Bestard-Cuche, Sarah Jäkel, Kazakou, Nina-Lydia, Sunniva M. K. Bøstrand, Laura J. Wagstaff, Cholewa-Waclaw, Justyna, Alastair Morris Kilpatrick, David van Bruggen, Mukund Kabbe et al. · 2024 · Figshare
- Additional file 6 of Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS functionLuise A. Seeker, Nadine Bestard-Cuche, Sarah Jäkel, Kazakou, Nina-Lydia, Sunniva M. K. Bøstrand, Laura J. Wagstaff, Cholewa-Waclaw, Justyna, Alastair Morris Kilpatrick, David van Bruggen, Mukund Kabbe et al. · 2024 · Figshare
- Additional file 1 of Bulk brain tissue cell-type deconvolution with bias correction for single-nuclei RNA sequencing data using DeTREMNicholas K. O’Neill, Thor D. Stein, Junming Hu, Habbiburr Rehman, Joshua David Campbell, Masanao Yajima, Xiaoling Zhang, Lindsay A Farrer · 2023 · Figshare
- Additional file 5 of Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS functionLuise A. Seeker, Nadine Bestard-Cuche, Sarah Jäkel, Kazakou, Nina-Lydia, Sunniva M. K. Bøstrand, Laura J. Wagstaff, Cholewa-Waclaw, Justyna, Alastair Morris Kilpatrick, David van Bruggen, Mukund Kabbe et al. · 2024 · Figshare
- Additional file 3 of Brain matters: unveiling the distinct contributions of region, age, and sex to glia diversity and CNS functionLuise A. Seeker, Nadine Bestard-Cuche, Sarah Jäkel, Kazakou, Nina-Lydia, Sunniva M. K. Bøstrand, Laura J. Wagstaff, Cholewa-Waclaw, Justyna, Alastair Morris Kilpatrick, David van Bruggen, Mukund Kabbe et al. · 2024 · Figshare
- Additional file 1 of Spatially resolved transcriptomics reveals genes associated with the vulnerability of middle temporal gyrus in Alzheimer’s diseaseChen, Shuo, Chang, Yuzhou, Liangping Li, Diana M. Acosta, Li, Yang, Qi Guo, Wang, Cankun, Emir Turkes, Morrison, Cody, Dominic L. Julian et al. · 2023 · Figshare
- Aging-associated alternative splicing programs conserved between human and mouse tissuesKeren Isaev, David A. Knowles · 2025 · bioRxiv (Cold Spring Harbor Laboratory)
- Spatial mapping of cellular and molecular plasticity in the maternal and postpartum mouse brainKeon Arbabi, Shahrzad Ghazisaeidi, Minjin Kim, Bharti Kukreja, Min Yi Feng, Spenser Pogue, Hugo Hudson, Maria Eleni Fafouti, Elizabeth L. Crouch, Liisa A.M. Galea et al. · 2026 · bioRxiv (Cold Spring Harbor Laboratory)
- The m6A writer VIRMA regulates the developmental elimination of retinal astrocytes and retinal vascular integrity maintenanceCuiting Wu, Xiaoli Wu, Min Wu, Xidan Zhou, Jie Tu, Bin Shen, Tao Zhou · 2026 · Neural Regeneration Research
- Epithelial and stromal remodelling in murine Crohn’s disease-like ileitisKatherine C. Letai, Bianca N. Islam, Paola Menghini, Carlo De Salvo, Neha S Khandekar, Bruce W Armstrong, Alka Tomar, Kimberly Curry, Theresa T. Pizarro, Paul J. Tesar et al. · 2026 · Gut Science
- Implication de nouvelles cibles microgliales, Cst7 et Clec7a/Dectin-1, dans le développement et la progression de la maladie d'AlzheimerAnne-Laure Hemonnot-Girard · 2021 · theses.fr (ABES)
- Mechanisms controlling microglial colonization of the forebrainCécile Bridlance · 2023 · theses.fr (ABES)
- Post‐Mortem Delay Induces Distinct Transcriptional and Morphological Changes in Microglia With Age‐Specific PatternsStanislav O. Kozlov, Eduard Schmidt, Heidi Theis, Elena De Domenico, Marc Beyer, Annett Halle · 2026 · Glia
- Influence des neurones sensoriels sur la croissance tumorale et sur la réponse immunitaire anti-tumorale associéeUgo Mardelle · 2024
- Comprehensive single cell profiling of ageing glial cells reveals impaired Wnt signalling and Jun transcription factors regulating cortical astrocytesMaroussia Hennes, Manja Thorwirth, Chu Lan Lao, Jeffrey A. Stogsdill, Paola Arlotta, Judith Fischer-Sternjak, Maria Lucia Richter, Magdalena Götz · 2026 · bioRxiv (Cold Spring Harbor Laboratory)
- Single-cell transcriptomics of human brain disordersKeon Arbabi, Giuseppe Carito, Tain Luquez, Michael Wainberg · 2026 · Molecular Psychiatry
- Immunization with Mycobacterium vaccae attenuates age-associated transcriptional remodeling and microglial reactivity in the rat hippocampusSteven P. Guerin, Kevin Sanchez, Arshia Verma, Nihal A. Salem, Roy Dayne Mayfield, Laura K. Fonken · 2026 · Journal of Neuroimmunology
- Adapted Smart-seq3xpress Facilitates Selective Microglial Transcriptomic Profiling From Frozen Brain TissueDominika Dostalova, Pavel Abaffy, Eva Rohlová, Ján Kriška, Tomáš Knotek, Jana Turečková, Denisa Kirdajová, Miroslava Anděrová, Lukáš Valihrach · 2026 · Cellular and Molecular Neurobiology
- Transcriptomic signatures of hippocampal active place avoidance memory maintenanceIsaac Vingan, Shwetha Phatarpekar, Victoria Sook Keng Tung, Alejandro Ivan Hernandez, Oleg Vadimovich Evgrafov, Juan Marcos Alarcón · 2026 · Frontiers in Cellular Neuroscience
- A GPCR atlas across human microglial states in Alzheimer's disease: Insights from snRNA-seq and hiPSC modelsBing Zhang, Ran Zhao, Xiao-Yan Li, Zi-Han Wei, Dong-Mei Wang, Mei-Hong Lu · 2026 · Experimental Neurology
- Single-cell fixed RNA profiling of the osteosarcoma clinical Spectrum identifies ADAMTS12 and RNASE1 as key drivers of the metastatic nicheNguyen Van Khanh, Trần Trung Dũng, Hoang Hai, Nguyen Tran Quang Sang, Tran Duc Thanh, Dang Minh Quang, Nguyen Thi Ha, Nguyen Bui Tam Chi, Pham Tuan Anh, Pham Minh Duc et al. · 2026 · Journal of bone oncology
- Forkhead box versus NF-κB hippocampal snRNA-seq profiles distinguish anti-Drebrin- and anti-GAD65-positive encephalitisKaren M. J. van Loo, Daniel S. Galvis-Montes, Annika Breuer, Juliane L Berns, Chiara A. Hummel, Tobias Res Baumgartner, Moritz Freyberg, Katharina M Mair, Jan Bauer, Theodor Rüber et al. · 2026 · Journal of Neuroinflammation
- Neuroinflammation as molecular landscape of post-operative delirium revealed by live human brain multi-omics profilingTakaya Ishii, Tao Wang, Kazuki Shibata, Shota Nishitani, Takehiko Yamanashi, Nadia E. Wahba, Tomoteru Seki, Kaitlyn J. Thompson, Kyosuke Yamanishi, Tsuyoshi Nishiguchi et al. · 2026 · Molecular Psychiatry
- Single-nucleus transcriptomic atlas of the goat lung characterizes cell types associated with Pasteurella multocida infectionQi An, Lianjie Liao, Haoju Pan et al. · 2026 · Microbial Pathogenesis
- The G-protein coupled receptor GPR34 promotes the homeostatic state of microglia and restrains the disease-associated microglial response in an Alzheimer’s disease modelAna Geller, Min Jee Kwon, Sean Simmons, Qihong Xu, William E. Martenis, Jordan L. Doman, Sahana Natarajan, Katherine J. Stalnaker, Jixiang Zhang, Xian Adiconis et al. · 2026 · eLife
- The G-protein coupled receptor GPR34 promotes the homeostatic state of microglia and restrains the disease-associated microglial response in an Alzheimer’s disease modelAna Geller, Min Jee Kwon, Sean Simmons, Qihong Xu, William E. Martenis, Jordan L. Doman, Sahana Natarajan, Katherine J. Stalnaker, Jixiang Zhang, Xian Adiconis et al. · 2026 · eLife
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- ROS-xCT metabolic coupling defines an astrocyte subtype that drives glutamate excitotoxicity during Alzheimer's disease progressionXueman Xie, Wenjing Liu, Longchao Chen, Jin Chen, Yiling Jiang, Qin Liu, Shouwen Chen · 2026 · Life Sciences
- Spreading alpha-synuclein oligomers trigger astrocyte/microglial changes and astrocyte-glutamatergic neuron system dysfunction in an age-related mannerJaehyun LeeBae, Verena Bopp, Alicia Goreth, Veselin Grozdanov, Zoé Engels, Julia K. Kühlwein, Ermanno Gazzola, Daniel Rombach, Laura Meier, Leda Dimou et al. · 2026 · Molecular Neurodegeneration Advances
- Cystatin F as a neuroimmune effector in CNS inflammation and neurodegenerationKaitlin Murray, Kellie Fernandez, Thomas E. Lane · 2026 · Frontiers in Cell and Developmental Biology
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- The microglial lineage: from molecular states to context-dependent functionsCuihua Li, Zhisheng Wu, Xianyuan Xiang, Hanjie Li · 2026 · Cell Insight