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Cited by 461Wastewater Treatment and Nitrogen RemovalMicrobial bioremediation and biosurfactantsWastewater Treatment and Reuse

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Bioaugmentation and its application in wastewater treatment: A review

Herrero, M., Stuckey, D.C.·2015·Chemosphere
DOI 10.1016/j.chemosphere.2014.10.033
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Abstract

Bioaugmentation (the process of adding selected strains/mixed cultures to wastewater reactors to improve the catabolism of specific compounds, e.g. refractory organics, or overall COD) is a promising technique to solve practical problems in wastewater treatment plants, and enhance removal efficiency. The potential of this option can now be enhanced in order to take advantage of important advances in the fields of microbial ecology, molecular biology, immobilization techniques and advanced bioreactor design. Reports on bioaugmentation in WWT show the difficulties in evaluating the potential parameters involved, leading frequently to inconclusive outcomes. Many studies have been carried out on the basis of trial-and-error approaches, and it has been reported that reactors bioaugmented with pure cultures often fail to perform as well as the pure cultures under laboratory conditions. As an interesting technical challenge, the feasibility of bioaugmentation should ultimately be assessed by data from field implementation, and this review highlights several promising areas to explore in the future.

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Research works printed but not linked: 66

  1. Ayala-del-Río, 2004, Correspondence between community structure and function during succession in phenol- and phenol-plus-trichloroethene-fed sequencing batch reactors, Appl. Environ. Microbiol., 70, 4950, 10.1128/AEM.70.8.4950-4960.2004 · DOI
  2. Barber, 1999, The use of the anaerobic baffled reactor (ABR) for wastewater treatment: a review, Water Res., 33, 1559, 10.1016/S0043-1354(98)00371-6 · DOI
  3. Bartroli, 2011, Bioaugmentation as a tool for improving the start-up and stability of a pilot-scale partial nitrification biofilm airlift reactor, Bioresour. Technol., 102, 4370, 10.1016/j.biortech.2010.12.084 · DOI
  4. Bhattad, U.H., Maki, J.S., Struble, C.A., Schauer-Gimenez, A.E., Zitomer, D.H., 2010. Culture conditions and cryoprotectant addition influences methanogenic activity after freeze-drying in air. In: Proceedings of International Water Association (IWA) 12th World Congress on Anaerobic Digestion, Guadalajara, Mexico, October 31st–November 4th, 5p.
  5. Boon, 2000, Bioaugmentation of activated sludge by an indigenous 3-chloroaniline-degrading Comamonas testosteroni strain, I2gfp, Appl. Environ. Microbiol., 66, 2906, 10.1128/AEM.66.7.2906-2913.2000 · DOI
  6. Boon, 2002, Bioaugmenting bioreactors for the continuous removal of 3-chloroaniline by a slow release approach, Environ. Sci. Technol., 36, 4698, 10.1021/es020076q · DOI
  7. Bouchez, 2000, Ecological study of a bioaugmentation failure, Environ. Microbiol., 2, 179, 10.1046/j.1462-2920.2000.00091.x · DOI
  8. Brar, 2010, Engineered nanoparticles in wastewater and wastewater sludge – evidence and impacts, Waste Manage., 30, 504, 10.1016/j.wasman.2009.10.012 · DOI
  9. Curtis, 2003, Theoretical ecology for engineering biology, Environ. Sci. Technol., 37, 64A, 10.1021/es0323493 · DOI
  10. Dejonghe, 2001, Bioaugmentation of soils by increasing microbial richness: missing links, Environ. Microbiol., 3, 649, 10.1046/j.1462-2920.2001.00236.x · DOI
  11. Díaz, 2010, Application of flow cytometry to industrial microbial bioprocesses, Biochem. Eng. J., 48, 385, 10.1016/j.bej.2009.07.013 · DOI
  12. Duran, 2006, Bioaugmenting anaerobic digestion of biosolids with selected strains of Bacillus, Pseudomonas, and Actinomycetes species for increased methanogenesis and odor control, Appl. Microbiol. Biotechnol., 73, 960, 10.1007/s00253-006-0548-6 · DOI
  13. El Fantroussi, 2005, Is bioaugmentation a feasible strategy for pollutant removal and site remediation?, Curr. Opin. Microbiol., 8, 268, 10.1016/j.mib.2005.04.011 · DOI
  14. Fernandez, 2000, Flexible community structure correlates with stable community function in methanogenic bioreactor communities perturbed by glucose, Appl. Environ. Microbiol., 66, 4058, 10.1128/AEM.66.9.4058-4067.2000 · DOI
  15. Fouratt, 2003, Application of temperature gradient gel electrophoresis to the characterization of a nitrifying bioaugmentation product, FEMS Microbiol. Ecol., 43, 277, 10.1111/j.1574-6941.2003.tb01068.x · DOI
  16. Fu, 2009, A bioaugmentation failure caused by phage infection and weak biofilm formation ability, J. Environ. Sci., 21, 1153, 10.1016/S1001-0742(08)62396-7 · DOI
  17. George, 2010, Extraction of monoclonal antibodies (IgG1) using anionic and anionic/nonionic reverse micelles, Biotechnol. Prog., 26, 1352, 10.1002/btpr.453 · DOI
  18. Guo, 2010, Application of bioaugmentation in the rapid start-up and stable operation of biological processes for municipal wastewater treatment at low temperatures, Bioresour. Technol., 101, 6622, 10.1016/j.biortech.2010.03.093 · DOI
  19. Hai, 2011, Bioaugmented membrane bioreactor (MBR) with a GAC-packed zone for high rate textile wastewater treatment, Water Res., 45, 2199, 10.1016/j.watres.2011.01.013 · DOI
  20. Hu, 2008, Biotreatment of ρ-nitrophenol and nitrobenzene in mixed wastewater through selective bioaugmentation, Bioresour. Technol., 99, 4529, 10.1016/j.biortech.2007.08.039 · DOI
  21. Ingber, 2010, From cellular mechanotransduction to biologically inspired engineering, Ann. Biomed. Eng., 38, 1148, 10.1007/s10439-010-9946-0 · DOI
  22. Jorgensen, 1999, Thioploca spp.: filamentous sulfur bacteria with nitrate vacuoles, FEMS Microbiol. Ecol., 28, 301, 10.1016/S0168-6496(98)00122-6 · DOI
  23. Lasken, 2012, Genomic sequencing of uncultured microorganisms from single cells, Nat. Rev. Microbiol., 10, 631, 10.1038/nrmicro2857 · DOI
  24. Lawrence, 1970, Unified basis for biological treatment design and operation, J. Sanit. Eng. Div. Am. Soc. Civ. Eng., 96, 757, 10.1061/JSEDAI.0001126 · DOI
  25. Lenz, 2009, Bioaugmentation of UASB reactors with immobilized Sulfurospirillum barnesii for simultaneous selenate and nitrate removal, Appl. Microbiol. Biotechnol., 83, 377, 10.1007/s00253-009-1915-x · DOI
  26. Logares, 2012, Environmental microbiology through the lens of high-throughput DNA sequencing: synopsis of current platforms and bioinformatics approaches, J. Microbiol. Methods, 91, 106, 10.1016/j.mimet.2012.07.017 · DOI
  27. Ma, 2009, Application of bioaugmentation to improve the activated sludge system into the contact oxidation system treating petrochemical wastewater, Bioresour. Technol., 100, 597, 10.1016/j.biortech.2008.06.066 · DOI
  28. Maphosa, 2010, Exploiting the ecogenomics toolbox for environmental diagnostics of organohalide-respiring bacteria, Trends Biotechnol., 28, 308, 10.1016/j.tibtech.2010.03.005 · DOI
  29. McClure, 1991, Genetic engineering for wastewater treatment, J. Inst. Water Environ. Manage., 5, 608, 10.1111/j.1747-6593.1991.tb00678.x · DOI
  30. Mertens, 2005, Stereospecific effect of hexachlorocyclohexane on activity and structure of soil methanotrophic communities, Environ. Microbiol., 7, 660, 10.1111/j.1462-2920.2005.00735.x · DOI
  31. Mohan, 2005, Bioaugmentation of an anaerobic sequencing batch biofilm reactor (AnSBBR) with immobilized sulphate reducing bacteria (SRB) for the treatment of sulphate bearing chemical wastewater, Process Biochem., 40, 2849, 10.1016/j.procbio.2004.12.027 · DOI
  32. Morris, 2014, Methyl coenzyme M reductase (mcrA) gene abundance correlates with activity measurements of methanogenic H2/CO2 enriched anaerobic biomass, Microb. Biotechnol., 7, 77, 10.1111/1751-7915.12094 · DOI
  33. Muttray, 2001, Population dynamics and metabolic activity of Pseudomonas abietaniphila BKME-9 within pulp mill wastewater microbial communities assayed by competitive PCR and RT-PCR, FEMS Microbiol. Ecol., 38, 21, 10.1111/j.1574-6941.2001.tb00878.x · DOI
  34. Nebe-von Caron, 2000, Analysis of bacterial function by multicolour fluorescence flow cytometry and single cell sorting, J. Microbiol. Methods, 42, 97, 10.1016/S0167-7012(00)00181-0 · DOI
  35. Ochman, 2000, Lateral gene transfer and the nature of bacterial innovation, Nature, 405, 299, 10.1038/35012500 · DOI · Paper page
  36. Ohandja, 2007, Biodegradation of PCE in a hybrid membrane aerated biofilm reactor, J. Environ. Eng., 133, 20, 10.1061/(ASCE)0733-9372(2007)133:1(20) · DOI
  37. Park, 2008, Bioaugmentation of cyanide-degrading microorganisms in a full-scale cokes wastewater treatment facility, Bioresour. Technol., 99, 2092, 10.1016/j.biortech.2007.03.027 · DOI
  38. Patureau, 2001, Combined phosphate and nitrogen removal in a sequencing batch reactor using the aerobic denitrifier, Microvirgula aerodenitrificans, Water Res., 35, 189, 10.1016/S0043-1354(00)00244-X · DOI
  39. Plaza, 2001, Impact of seeding with nitrifying bacteria on nitrification process efficiency, Water Sci. Technol., 43, 155, 10.2166/wst.2001.0038 · DOI
  40. Qu, 2009, Population dynamics in bioaugmented membrane bioreactor for treatment of bromoamine acid wastewater, Bioresour. Technol., 100, 244, 10.1016/j.biortech.2008.06.023 · DOI
  41. Rostron, 2001, Nitrification of high strength ammonia wastewater: comparative study of immobilization media, Water Res., 35, 1169, 10.1016/S0043-1354(00)00365-1 · DOI
  42. Saravanane, 2001, Bioaugmentation and treatment of cephalexin drug-based pharmaceutical effluent in an up-flow anaerobic fluidized bed system, Bioresour. Technol., 76, 279, 10.1016/S0960-8524(00)00121-8 · DOI
  43. Schauer-Gimenez, 2010, Bioaugmentation for improved recovery of anaerobic digesters after toxicant exposure, Water Res., 44, 3555, 10.1016/j.watres.2010.03.037 · DOI
  44. Shan, 2009, Application of nanomaterials in environmental science and engineering: review, Pract. Period Hazard. Toxic Radioact. Waste Manage., 13, 110, 10.1061/(ASCE)1090-025X(2009)13:2(110) · DOI
  45. Singer, 2005, Perspectives and vision for strain selection in bioaugmentation, Trends Biotechnol., 23, 74, 10.1016/j.tibtech.2004.12.012 · DOI
  46. Sloan, 2006, Quantifying the roles of immigration and chance in shaping prokaryote community structure, Environ. Microbiol., 8, 732, 10.1111/j.1462-2920.2005.00956.x · DOI
  47. Stephenson, 1992, Bioaugmentation for enhancing biological wastewater treatment, Biotechnol. Adv., 10, 549, 10.1016/0734-9750(92)91452-K · DOI
  48. Tale, 2011, Methanogen community structure–activity relationship and bioaugmentation of overloaded anaerobic digesters, Water Res., 45, 5249, 10.1016/j.watres.2011.07.035 · DOI
  49. Taly, 2007, Droplets as microreactors for high-throughput biology, ChemBioChem, 8, 263, 10.1002/cbic.200600425 · DOI
  50. Thompson, 2005, Bioaugmentation for bioremediation: the challenge of strain selection, Environ. Microbiol., 7, 909, 10.1111/j.1462-2920.2005.00804.x · DOI
  51. Top, 2002, Catabolic mobile genetic elements and their potential use in bioaugmentation of polluted soils and waters, FEMS Microbiol. Ecol., 42, 199, 10.1111/j.1574-6941.2002.tb01009.x · DOI
  52. Tsutsui, 2010, Transfer of plasmid pJP4 from Escherichia coli and Pseudomonas putida to bacteria in activated sludge developed under different sludge retention times, J. Biosci. Bioeng., 110, 684, 10.1016/j.jbiosc.2010.07.009 · DOI
  53. Van der Gast, 2002, Selection of microbial consortia for treating metal working fluids, J. Ind. Microbiol. Biotechnol., 29, 20, 10.1038/sj.jim.7000271 · DOI
  54. Van der Gast, 2004, Temporal dynamics and degradation activity of a bacterial inoculum for treating waste metal-working fluid, Environ. Microbiol., 6, 254, 10.1111/j.1462-2920.2004.00566.x · DOI
  55. Van Veen, 1997, Fate and activity of microorganisms introduced into soil, Microbiol. Mol. Biol. Rev., 61, 121, 10.1128/.61.2.121-135.1997 · DOI
  56. Verstraete, 2007, Microbial ecology and environmental biotechnology, ISME J., 1, 4, 10.1038/ismej.2007.7 · DOI
  57. Vyrides, 2010, Are compatible solutes compatible with biological treatment of saline wastewater? Batch and continuous studies using submerged anaerobic membrane bioreactors (SAMBRs), Environ. Sci. Technol., 44, 7437, 10.1021/es903981k · DOI
  58. Wang, 2010, Single cell analysis: the new frontier in ‘omics’, Trends Biotechnol., 28, 281, 10.1016/j.tibtech.2010.03.002 · DOI
  59. Wang, 2009, Bioaugmentation with the nicotine-degrading bacterium Pseudomonas sp. HF-1 in a sequencing batch reactor treating tobacco wastewater: degradation study and analysis of its mechanisms, Water Res., 43, 4187, 10.1016/j.watres.2009.07.012 · DOI
  60. Watanabe, 2002, Stable bioaugmentation of activated sludge with foreign catabolic genes harboured by an indigenous dominant bacterium, Environ. Microbiol., 4, 577, 10.1046/j.1462-2920.2002.00342.x · DOI
  61. Wenderoth, 2003, Bacterial community dynamics during biostimulation and bioaugmentation experiments aiming at chlorobenzene degradation in groundwater, Microb. Ecol., 46, 161, 10.1007/s00248-003-2005-8 · DOI
  62. Wittebolle, 2005, Failure of the ammonia oxidation process in two pharmaceutical wastewater treatment plants is linked to shifts in the bacterial communities, J. Appl. Microbiol., 99, 997, 10.1111/j.1365-2672.2005.02731.x · DOI
  63. Wittebolle, 2008, Quantifying community dynamics of nitrifiers in functionally stable reactors, Appl. Environ. Microbiol., 74, 286, 10.1128/AEM.01006-07 · DOI
  64. Yu, 2002, Bioaugmentation with the resin-acid degrading bacteria Zoogloea resiniphila DhA-35 to counteract pH stress in an aerated lagoon treating pulp and paper mill effluent, Water Res., 36, 2793, 10.1016/S0043-1354(01)00496-1 · DOI
  65. Yu, 2010, Bioaugmentation of a sequencing batch reactor with Pseudomonas putida ONBA-17, and its impact on reactor bacterial communities, J. Hazard. Mater., 176, 20, 10.1016/j.jhazmat.2009.06.006 · DOI
  66. Zhou, 2002, Challenges in applying microarrays to environmental studies, Curr. Opin. Biotechnol., 13, 204, 10.1016/S0958-1669(02)00319-1 · DOI

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Metadata

Authors
Herrero, M., & Stuckey, D. C.
Affiliations
  • Universidad de Oviedo (ES)
  • Imperial College London (GB)
Corresponding author
Stuckey, D.C.
ORCID
Venue
Chemosphere
Publisher
Elsevier BV
Published
2015-12
Volume
140
Pages
119-128
ISSN
0045-6535 (ISSN-L)
DOI
10.1016/j.chemosphere.2014.10.033
OpenAlex
W2036633918
Cited by
461
References
0
Citations by year
2026: 40 · 2025: 28 · 2024: 40 · 2023: 44 · 2022: 57 · 2021: 59 · 2020: 44 · 2019: 30 · 2018: 45 · 2017: 37 · 2016: 27 · 2015: 9
FWCI
1.67
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Chemosphere (publishedVersion)
  • PubMed (publishedVersion)
Funders
Biotechnology and Biological Sciences Research Council (BB/K003240/2)
Topics
  • Physical Sciences › Environmental Science › Pollution › Wastewater Treatment and Nitrogen Removal
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Physical Sciences › Environmental Science › Waste Management and Disposal › Wastewater Treatment and Reuse
Keywords
bioaugmentation, wastewater treatment, recalcitrant organic matter, COD removal, microbial ecology, microbial immobilization, bioreactor design, field implementation, wastewater treatment plants
MeSH terms
Biodegradation, Environmental, Waste Disposal, Fluid, Bioreactors, Wastewater

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Herrero, M., & Stuckey, D. C. (2015). Bioaugmentation and its application in wastewater treatment: A review. Chemosphere. https://doi.org/10.1016/j.chemosphere.2014.10.033