CoChat
Sign Up
Cited by 62Genetically Modified Organisms ResearchPharmaceutical and Antibiotic Environmental ImpactsPesticide and Herbicide Environmental Studies

The DOI matches its Crossref record.

Adsorption capacity of multiple DNA sources to clay minerals and environmental soil matrices less than previously estimated

Open access
Gardner, Courtney M., Gunsch, Claudia K.·2017·Chemosphere
DOI 10.1016/j.chemosphere.2017.02.030
Open this paper in CoChat — free account

Abstract

The cultivation and consumption of transgenic crops continues to be a widely debated topic, as the potential ecological impacts are not fully understood. In particular, because antibiotic resistance genes (ARGs) have historically been used as selectable markers in the genetic engineering of transgenic crops, it is important to determine if the genetic constructs found in decomposing transgenic crops persist long enough in the environment and if they can be transferred horizontally to indigenous microorganisms. In the present study, we address the question of persistence. Others have also estimated the DNA adsorption capacity of various clays, but have done so by manipulating the surface charge and size of particles tested which may overestimate sorption and underestimate the DNA available for horizontal transfer. In the present study, isotherms were generated using model Calf Thymus DNA and transgenic maize DNA without surface modification. Montmorillonite, kaolinite, and 3 soil mixtures with varying clay content were used in this study. The adsorption capacity of pure montmorillonite and kaolinite minerals was found to be one to two orders of magnitude less than previously estimated likely due to the distribution of clay particle sizes and heteroionic particle surface charge. However, it appears that a substantial amount of DNA is still able to adsorb onto these matrices (up to 200 mg DNA per gram of clay) suggesting the potential availability of free transgenic DNA in the e…

Overview

Integrity signals

Registry

Crossref · 10.1016/j.chemosphere.2017.02.030

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 38

  1. Aeschbacher, 2005, Bt176 corn in poultry nutrition: physiological characteristics and fate of recombinant plant DNA in chickens, Poult. Sci., 84, 385, 10.1093/ps/84.3.385 · DOI
  2. Allen, 2010, Call of the wild: antibiotic resistance genes in natural environments, Nat. Rev. Microbiol., 8, 251, 10.1038/nrmicro2312 · DOI · Paper page
  3. Bennett, 2004, An assessment of the risks associated with the use of antibiotic resistance genes in genetically modified plants: report of the Working Party of the British Society for Antimicrobial Chemotherapy, J. Antimicrob. Chemother., 53, 418, 10.1093/jac/dkh087 · DOI
  4. Cehovin, 2008, Specific DNA recognition mediated by a type IV pilin, Proc. Natl. Acad. Sci. U. S. A., 110, 3065, 10.1073/pnas.1218832110 · DOI
  5. Chalikian, 1999, A more unified picture for the thermodynamics of nucleic acid duplex melting: a characterization by calorimetric and volumetric techniques, Proc. Natl. Acad. Sci., 96, 7853, 10.1073/pnas.96.14.7853 · DOI
  6. Chen, 2004, DNA uptake during bacterial transformation, Nat. Rev. Microbiol., 2, 241, 10.1038/nrmicro844 · DOI
  7. Chen, 2008, Mechanism of homologous recombination from the RecA–ssDNA/dsDNA structures, Nature, 453, 484, 10.1038/nature06971 · DOI
  8. Chopra, 2001, Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance, Microbiol. Mol. Biol. Rev., 65, 232, 10.1128/MMBR.65.2.232-260.2001 · DOI · Paper page
  9. Cianciotto, 2005, Type II secretion: a protein secretion system for all seasons, Trends Microbiol., 13, 581, 10.1016/j.tim.2005.09.005 · DOI
  10. Clausen-Schaumann, 2000, Mechanical stability of single DNA molecules, Biophys. J., 78, 1997, 10.1016/S0006-3495(00)76747-6 · DOI
  11. Conner, 2003, The release of genetically modified crops into the environment, Plant J., 33, 19, 10.1046/j.0960-7412.2002.001607.x · DOI
  12. Demanèche, 2001, Evaluation of biological and physical protection against nuclease degradation of clay-bound plasmid DNA, Appl. Environ. Microbiol., 67, 293, 10.1128/AEM.67.1.293-299.2001 · DOI
  13. Dunfield, 2004, Impact of genetically modified crops on soil-and plant-associated microbial communities, J. Environ. Qual., 33, 806, 10.2134/jeq2004.0806 · DOI
  14. Giger, 2003, Occurrence and fate of antibiotics as trace contaminants in wastewaters, sewage sludges, and surface waters, CHIMIA Int. J. Chem., 57, 485, 10.2533/000942903777679064 · DOI
  15. Goossens, 2005, Outpatient antibiotic use in Europe and association with resistance: a cross-national database study, Lancet, 365, 579, 10.1016/S0140-6736(05)17907-0 · DOI · Paper page
  16. Haberer, 2005, Structure and architecture of the maize genome, Plant Physiol., 139, 1612, 10.1104/pp.105.068718 · DOI
  17. Iamtham, 2000, Removal of antibiotic resistance genes from transgenic tobacco plastids, Nat. Biotechnol., 18, 1172, 10.1038/81161 · DOI
  18. Johnsborg, 2007, Natural genetic transformation: prevalence, mechanisms and function, Res. Microbiol., 158, 767, 10.1016/j.resmic.2007.09.004 · DOI
  19. Khachatourians, 1998, Agricultural use of antibiotics and the evolution and transfer of antibiotic-resistant bacteria, Can. Med. Assoc. J., 159, 1129
  20. Khanna, 1992, Transformation of Bacillus subtilis by DNA bound on montmorillonite and effect of DNase on the transforming ability of bound DNA, Appl. Environ. Microbiol., 58, 1930, 10.1128/aem.58.6.1930-1939.1992 · DOI
  21. Knapp, 2010, Evidence of increasing antibiotic resistance gene abundances in archived soils since 1940, Environ. Sci. Technol., 44, 580, 10.1021/es901221x · DOI
  22. Lacks, 1974, Role of a deoxyribonuclease in the genetic transformation of Diplococcus pneumonia, Proc. Natl. Acad. Sci. U. S. A., 71, 2305, 10.1073/pnas.71.6.2305 · DOI
  23. Livermore, 1995, Beta-Lactamases in laboratory and clinical resistance, Clin. Microbiol. Rev., 8, 557, 10.1128/CMR.8.4.557 · DOI
  24. Lorenz, 1987, Adsorption of DNA to sand and variable degradation rates of adsorbed DNA, Appl. Environ. Microbiol., 53, 2948, 10.1128/aem.53.12.2948-2952.1987 · DOI
  25. Magee, 1999, Antibiotic prescribing and antibiotic resistance in community practice: retrospective study, 1996-8, Bmj, 319, 1239, 10.1136/bmj.319.7219.1239 · DOI
  26. Miao, 1970, Competent Diplococcus pneumoniae accept both single-and double-stranded deoxyribonucleic acid, J. Bacteriol., 101, 361, 10.1128/jb.101.2.361-364.1970 · DOI
  27. Mortier-Barrière, 2007, A key presynaptic role in transformation for a widespread bacterial protein: DprA conveys incoming ssDNA to RecA, Cell, 130, 824, 10.1016/j.cell.2007.07.038 · DOI
  28. Neuhauser, 2003, Antibiotic resistance among gram-negative bacilli in US intensive care units: implications for fluoroquinolone use, J. Am. Med. Assoc., 289, 885, 10.1001/jama.289.7.885 · DOI
  29. Ogram, 1988, DNA adsorption to soils and sediments, Environ. Sci. Technol., 22, 982, 10.1021/es00173a020 · DOI
  30. Paget, 1992, Adsorption of DNA on clay minerals: protection against DNaseI and influence on gene transfer, FEMS Microbiol. Lett., 97, 31, 10.1111/j.1574-6968.1992.tb05435.x · DOI
  31. Pietramellara, 2009, Extracellular DNA in soil and sediment: fate and ecological relevance, Biol. Fertil. Soils, 45, 219, 10.1007/s00374-008-0345-8 · DOI
  32. Poly, 2000, Differences between linear chromosomal and supercoiled plasmid DNA in their mechanisms and extent of adsorption on clay minerals, Langmuir, 16, 1233, 10.1021/la990506z · DOI
  33. Riesenfeld, 2004, Uncultured soil bacteria are a reservoir of new antibiotic resistance genes, Environ. Microbiol., 6, 981, 10.1111/j.1462-2920.2004.00664.x · DOI
  34. Romanowski, 1991, Adsorption of plasmid DNA to mineral surfaces and protection against DNase I, Appl. Environ. Microbiol., 57, 1057, 10.1128/aem.57.4.1057-1061.1991 · DOI
  35. Simbolo, 2013, DNA qualification workflow for next generation sequencing of histopathological samples, PLoS One, 8, e62692, 10.1371/journal.pone.0062692 · DOI
  36. Thiele-Bruhn, 2003, Pharmaceutical antibiotic compounds in soils–a review, J. Plant Nutr. Soil Sci., 166, 145, 10.1002/jpln.200390023 · DOI
  37. Widmer, 1996, Sensitive detection of transgenic plant marker gene persistence in soil microcosms, Mol. Ecol., 5, 603, 10.1111/j.1365-294X.1996.tb00356.x · DOI
  38. Witte, 1998, Medical consequences of antibiotic use in agriculture, Science, 279, 996, 10.1126/science.279.5353.996 · DOI · Paper page

Cited by

Sorted by citations

Metadata

Authors
Gardner, C. M., & Gunsch, C. K.
Affiliations
Duke University (US)
Corresponding author
Gunsch, Claudia K.
ORCID
Venue
Chemosphere
Publisher
Elsevier BV
Published
2017-05
Volume
175
Pages
45-51
ISSN
0045-6535 (ISSN-L)
DOI
10.1016/j.chemosphere.2017.02.030
OpenAlex
W2585070954
Cited by
62
References
0
Citations by year
2026: 5 · 2025: 7 · 2024: 6 · 2023: 7 · 2022: 9 · 2021: 9 · 2020: 5 · 2019: 7 · 2018: 5 · 2017: 2
FWCI
4.95
Type
journal article
Language
en
Open access
Licence
Locations
  • Chemosphere (acceptedVersion)
  • PubMed (publishedVersion)
Funders
National Science Foundation (CBET-1438612, GRFP DGF-106401)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
  • Physical Sciences › Environmental Science › Pollution › Pharmaceutical and Antibiotic Environmental Impacts
  • Physical Sciences › Environmental Science › Pollution › Pesticide and Herbicide Environmental Studies
Keywords
DNA adsorption, clay minerals, montmorillonite, kaolinite, calf thymus DNA, transgene persistence, horizontal gene transfer, antibiotic resistance genes, particle surface charge
MeSH terms
Clay, Adsorption, Aluminum Silicates, Bentonite, Zea mays, DNA, Kaolin, Minerals, Soil, Soil Pollutants, Transgenes, Gene Transfer, Horizontal, Plants, Genetically Modified

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review