Characterization of a polycyclic aromatic ring-hydroxylation dioxygenase from Mycobacterium sp. NJS-P
Abstract
Ring-hydroxylating dioxygenases (RHDs) play a critical role in the biodegradation of polycyclic aromatic hydrocarbons (PAHs). In this study, genes pdoAB encoding a dioxygenase capable of oxidizing various PAHs with up to five-ring benzo[a]pyrene were cloned from Mycobacterium sp. NJS-P. The α-subunit of the PdoAB showed 99% and 93% identity to that from Mycobacterium sp. S65 and Mycobacterium sp. py136, respectively. An Escherichia coli expression experiment revealed that the enzyme is able to oxidize anthracene, phenanthrene, pyrene and benzo[a]pyrene, but not to fluoranthene and benzo[a]anthracene. Furthermore, the results of in silico analysis showed that PdoAB has a large substrate-binding pocket satisfying for accommodation of HMW PAHs, and suggested that the binding energy of intermolecular interaction may predict the substrate conversion of RHDs towards HMW PAHs, especially those may have steric constraints on the substrate-binding pocket, such as benzo[a]pyrene and benzo[a]anthracene.
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Zeng et al. demonstrated that the PdoAB dioxygenase from Mycobacterium sp. NJS-P performs diverse activities in the oxidation of several PAHs, including molecules with up to five rings, such as benzo[α]pyrene.
“Zeng et al. [397] demonstrated that the PdoAB dioxygenase from Mycobacterium sp.NJS-P performs diverse activities in the oxidation of several PAHs, including molecules with up to five rings, such as benzo[α]pyrene.”
From Harnessing bacterial consortia for effective bioremediation: targeted removal of heavy metals, hydrocarbons, and persistent pollutants (2025) · cites this paper as [397]
Cites
Dundas, J., Ouyang, Z., Tseng, J., Binkowski, A., Turpaz, Y., & Liang, J. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl282 · Cited by 1,725 · see citing works
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Qattan, S. Y. A. (2025) · Environmental Sciences Europe · doi:10.1186/s12302-025-01103-y · Cited by 109 · see citing works
- Authors
- Zeng, J., Zhu, Q., Wu, Y., Chen, H., & Lin, X.
- Affiliations
- Chinese Academy of Sciences (CN)
- Corresponding author
- Lin, Xiangui
- Venue
- Chemosphere
- Publisher
- Elsevier BV
- Published
- 2017-10
- Volume
- 185
- Pages
- 67-74
- ISSN
- 0045-6535 (ISSN-L)
- OpenAlex
- W2731234788
- Cited by
- 40
- References
- 1
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 5 · 2022: 8 · 2021: 7 · 2020: 3 · 2019: 6 · 2018: 5
- FWCI
- 1.66
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemosphere (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Key Basic Research Program of China (2014CB441106)
- National Natural Science Foundation of China (41201301, 41371310)
- Topics
- Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
- Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Steroid Chemistry and Biochemistry
- Keywords
- ring-hydroxylating dioxygenase, polycyclic aromatic hydrocarbons, benzo[a]pyrene, biodegradation, substrate binding pocket, substrate specificity, anthracene, phenanthrene, pyrene, fluoranthene, benz[a]anthracene, high molecular weight PAHs
- MeSH terms
- Anthracenes, Benzo(a)pyrene, Biodegradation, Environmental, Fluorenes, Hydroxylation, Mycobacterium, Phenanthrenes, Polycyclic Aromatic Hydrocarbons, Pyrenes, Dioxygenases
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