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Cited by 73Phytochemistry and biological activity of medicinal plantsChromatography in Natural ProductsTraditional Chinese Medicine Analysis

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Identification and quantification of the constituents of madder root by gas chromatography and high-performance liquid chromatography

Boldizsár, I., Szűcs, Z., Füzfai, Zs., Molnár-Perl, I.·2006·Journal of Chromatography A
DOI 10.1016/j.chroma.2006.08.021
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Abstract

The possibilities in the identification and quantitation of the constituents of Rubia tinctorum L.'s root, called also madder root, was described and compared by gas chromatography (GC)-MS, high-performance liquid chromatography (HPLC)-UV/photodiode array detection (DAD) and HPLC-MS: chromatographic analyses were carried out in parallel, from the same samples/extracts/hydrolyzates. Anthraquinone glycosides, anthraquinones, carboxylic acids and sugars were determined directly in the presence of the matrix and in its extracts without and subsequently to hydrolyses. Hydrolyses were performed as a function of time, with hydrochloric and trifluoroacetic acids, as well as enzymatically. Data revealed that as hydrolyzing agent trifluoroacetic acid is to be preferred. Madder root's anthraquinones (pseudopurpurin/purpurin, alizarin, lucidin, munjistin, nordamnacanthal) were identified on the basis of their absorption spectra (HPLC-DAD) and fragmentation patterns by GC-MS and HPLC-MS, equally. Reproducibility of anthraquinone's quantitation, by HPLC-DAD and GC-MS, in the concentration ranges of 4 x 10(-5) to 3 x 10(-2)g/g dried sample, provided an average reproducibility of 4.2% (varying between 0.9 and 9.4% relative standard deviation (RSD percentages)). Carboxylic acids (malic, citric, quinic, rosmarinic acids) and saccharides (xylose, ribose, fructose, glucose, sucrose, primverose) were quantified as their trimethylsilyl (oxime) ether/ester derivatives by GC-MS, in the concentration ranges of 10(-5)g to 10(-2)g/g dried sample, with an average reproducibility of 4.7% RSD.

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Metadata

Authors
Boldizsár, I., Szűcs, Z., Füzfai, Z., & Molnár-Perl, I.
Affiliations
Eötvös Loránd University (HU)
Corresponding author
Molnár-Perl, I.
ORCID
Boldizsár, I.
Venue
Journal of Chromatography A
Publisher
Elsevier BV
Published
2006-11
Volume
1133
Issue
1-2
Pages
259-274
ISSN
0021-9673 (ISSN-L)
DOI
10.1016/j.chroma.2006.08.021
OpenAlex
W2010206204
Cited by
73
References
0
Citations by year
2026: 1 · 2025: 3 · 2024: 2 · 2023: 2 · 2022: 1 · 2021: 3 · 2020: 2 · 2019: 3 · 2018: 3 · 2017: 8 · 2016: 1 · 2015: 2 · 2014: 6 · 2013: 2 · 2012: 4
FWCI
7.77
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Chromatography A (publishedVersion)
  • PubMed (publishedVersion)
Funders
Magyar Tudományos Akadémia
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and biological activity of medicinal plants
  • Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
  • Health Sciences › Medicine › Complementary and alternative medicine › Traditional Chinese Medicine Analysis
Keywords
madder root, Rubia tinctorum, anthraquinone glycosides, anthraquinones, purpurin, alizarin, lucidin, nordamnacanthal, carboxylic acids, sugar, acid hydrolysis, trifluoroacetic acid, enzymatic hydrolysis, quantification, reproducibility, trimethylsilyl derivatives
MeSH terms
Anthraquinones, Chromatography, Gas, Chromatography, High Pressure Liquid, Reproducibility of Results, Molecular Structure, Plant Roots, Rubia

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