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Foundations of retention in partition chromatography

Poole, Colin F., Poole, Salwa K.·2009·Journal of Chromatography A
DOI 10.1016/j.chroma.2008.10.092
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Abstract

The connection between the observable output in column chromatography (retention time, retention volume, retention factor, separation factor, etc.) and system properties (hold-up volume, pressure, temperature, isotherm behavior, etc.) is discussed from a practical and mechanistic perspective for gas-liquid chromatography, reversed-phase liquid chromatography, supercritical fluid chromatography, micellar electrokinetic chromatography, and capillary electrochromatography. The unifying feature of these techniques is that retention can be described by a partition model, although not always exclusively. When over simplistic system models are used to explain variation in retention parameters they frequently mask the true reasons for poor repeatability and difficulties in transfer between system. Methods employing relative retention afford higher precision but may contain residual uncorrected errors. For those systems with several separate mechanisms contributing to retention the effective retention parameters can no longer be interpreted by simple partition models. The broadly based and practically focused material in this article affords an illustration of the often complicated relationship between system properties and retention, and the dangers that lurk in simplified retention models if the validity of their underlining approximations is not appropriate for the system under study.

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  • For polar stationary phases, the contribution of interfacial adsorption to retention, in addition to the bulk partition mechanism, has been shown to be significant in some cases, which may be a reason for the observed nonlinearity.

    “For polar stationary phases, the contribution of interfacial adsorption to retention, in addition to the bulk partition mechanism, has been shown to be significant in some cases,30 which may be a reason for the observed nonlinearity.”

    From Intermolecular Interactions, Solute Descriptors, and Partition Properties of Neutral Per- and Polyfluoroalkyl Substances (PFAS) (2023) · cites this paper as [30]

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Metadata

Authors
Poole, C. F., & Poole, S. K.
Venue
Journal of Chromatography A
Publisher
Elsevier BV
Published
2009-03
Volume
1216
Issue
10
Pages
1530-1550
ISSN
0021-9673
DOI
10.1016/j.chroma.2008.10.092
Cited by
111
References
0
Language
en
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

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