Thin-layer chromatography with biological detection in phytochemistry
Abstract
Bioautography on thin-layer chromatographic (TLC) plates is a means of detecting the biological activity of a sample which has migrated on the plate with a suitable solvent. It only requires small amounts of sample and is ideal for the investigation of plant constituents, which often occur as complex mixtures. It can be used for the target-directed isolation of these constituents. In contrast to HPLC, many samples can be run at the same time on TLC. Organic solvents, which cause inactivation of enzymes or death of living organisms, can be completely removed before biological detection. Many bioassays are compatible with TLC. Antimicrobial, radical scavenging, antioxidant activities and enzyme inhibition feature among the tests that are employed.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
TLC-bioautography is a simple, effective and inexpensive technique for separation of a complex mixture and localizes active constituents on the TLC plate.
“Overall, TLC–bioautography is a simple, effective and inexpensive technique for the separation of a complex mixture, and at the same time, it localizes the active constituents on the TLC plate.”
From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [44]
Agar overlay bioassay provides well-defined growth inhibition zones and is not sensitive to contamination.
“It provides well-defined growth inhibition zones and is not sensitive to contamination [44].”
From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [44]
p-Iodonitrotetrazolium violet is the most suitable detection reagent for direct bioautography.
“p-Iodonitrotetrazolium violet is the most suitable detection reagent [44], [48].”
From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [44]
TLC-bioautography can be performed both in sophisticated laboratories and in small laboratories with access to a minimum of equipment.
“Therefore, it can be performed both in sophisticated laboratories and small laboratories which only have access to a minimum of equipment [44].”
From Methods for in vitro evaluating antimicrobial activity: A review (2016) · cites this paper as [44]
Cited by
Sorted by citations
Methods for in vitro evaluating antimicrobial activity: A review
Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016) · Journal of Pharmaceutical Analysis · doi:10.1016/j.jpha.2015.11.005 · Cited by 7,264 · see citing works
- Authors
- Marston, A.
- Affiliations
- University of the Free State (ZA)
- Corresponding author
- Marston, A.
- Venue
- Journal of Chromatography A
- Publisher
- Elsevier BV
- Published
- 2011-05
- Volume
- 1218
- Issue
- 19
- Pages
- 2676-2683
- ISSN
- 0021-9673 (ISSN-L)
- OpenAlex
- W2002848763
- Cited by
- 220
- References
- 0
- Citations by year
- 2026: 9 · 2025: 10 · 2024: 11 · 2023: 20 · 2022: 23 · 2021: 17 · 2020: 13 · 2019: 10 · 2018: 16 · 2017: 13 · 2016: 20 · 2015: 18 · 2014: 17 · 2013: 14 · 2012: 8
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Chromatography A (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Analytical Chemistry › Chromatography in Natural Products
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioactive natural compounds
- Keywords
- bioautography, biological detection, plant constituents, phytochemistry, bioassay-guided isolation, antimicrobial activity, antioxidant activity, free radical scavenging, enzyme inhibition, organic solvent removal
- MeSH terms
- Antioxidants, Biological Assay, Chromatography, Thin Layer, Enzyme Inhibitors, Microbial Sensitivity Tests, Plant Extracts, Free Radical Scavengers
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
