Rare-Event Analysis in Flow Cytometry
Abstract
Technical aspects of rare-event detection are discussed in this article in a practical context, with two real-life examples. A growing number of flow cytometry-based assays depend on rare-event detection for basic science and clinical applications.
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Each is checked against this paper’s own text: does it say what the citing paper says it does?
The record claimed in the literature for tumor cells spiked in peripheral blood is one cell in 10 million.
“The term “rare” generally refers to events with a frequency of 0.01% or less, although the record claimed in the literature has long stood at one cell in 10 million for tumor cells spiked in the peripheral blood [96, 97].”
From Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition) (2021) · cites this paper as [97]
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References 67 · Cited by 97
- Authors
- Donnenberg, A. D., & Donnenberg, V. S.
- Affiliations
- University of Pittsburgh (US)
- Corresponding author
- Donnenberg, Albert D.
- Venue
- Clinics in Laboratory Medicine
- Publisher
- Elsevier BV
- Published
- 2007-09
- Volume
- 27
- Issue
- 3
- Pages
- 627-652
- ISSN
- 0272-2712 (ISSN-L)
- OpenAlex
- W2075164322
- Cited by
- 97
- References
- 67
- Citations by year
- 2026: 4 · 2025: 1 · 2024: 7 · 2023: 7 · 2022: 8 · 2021: 6 · 2020: 3 · 2019: 4 · 2018: 1 · 2017: 7 · 2016: 8 · 2015: 3 · 2014: 5 · 2013: 4 · 2012: 5
- FWCI
- 0.61
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinics in Laboratory Medicine (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (N01 AR 92239)
- Topics
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Cell Image Analysis Techniques
- Physical Sciences › Mathematics › Statistics and Probability › Probability and Statistical Research
- Keywords
- rare event detection, flow cytometry
- MeSH terms
- Flow Cytometry, Humans, Sensitivity and Specificity, Reproducibility of Results, Immunophenotyping
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
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