Cell-Type-Resolved Quantitative Proteomics of Murine Liver
Open accessAbstract
Mass spectrometry (MS)-based proteomics provides a powerful approach to globally investigate the biological function of individual cell types in mammalian organs. Here, we applied this technology to the in-depth analysis of purified hepatic cell types from mouse. We quantified 11,520 proteins, making this the most comprehensive proteomic resource of any organ to date. Global protein copy number determination demonstrated that a large proportion of the hepatocyte proteome is dedicated to fatty acid and xenobiotic metabolism. We identified as-yet-unknown components of the TGF-β signaling pathway and extracellular matrix in hepatic stellate cells, uncovering their regulative role in liver physiology. Moreover, our high-resolution proteomic data set enabled us to compare the distinct functional roles of hepatic cell types in cholesterol flux, cellular trafficking, and growth factor receptor signaling. This study provides a comprehensive resource for liver biology and biomedicine.
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Data from several large-scale, high-resolution, FDR-controlled cellular proteome projects show coverage saturating at approximately 11,000.
“This analysis is supported by data from several large scale, high-resolution, FDR-controlled cellular proteome projects, where the coverage saturated at approximately 11,000 (refs. 15,18,45,46).”
From Cell type- and brain region-resolved mouse brain proteome (2015) · cites this paper as [46]
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References 56 · Cited by 173
- Authors
- Azimifar, S. B., Nagaraj, N., Cox, J., & Mann, M.
- Affiliations
- Max Planck Institute of Biochemistry (DE)
- Max Planck Society (DE)
- Corresponding author
- Mann, Matthias
- ORCID
- Mann, Matthias
- Venue
- Cell Metabolism
- Publisher
- Elsevier BV
- Published
- 2014-12
- Volume
- 20
- Issue
- 6
- Pages
- 1076-1087
- ISSN
- 1550-4131 (ISSN-L)
- OpenAlex
- W1967339738
- Cited by
- 173
- References
- 56
- Citations by year
- 2026: 5 · 2025: 16 · 2024: 14 · 2023: 14 · 2022: 15 · 2021: 14 · 2020: 23 · 2019: 16 · 2018: 16 · 2017: 19 · 2016: 14 · 2015: 7
- FWCI
- 5.71
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Max Planck Digital Library (submittedVersion)
- MPG.PuRe (Max Planck Society) (submittedVersion)
- Funders
- Bundesministerium für Bildung und Forschung
- Max-Planck-Gesellschaft
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Keywords
- liver proteome, hepatocytes, hepatic stellate cells, fatty acid metabolism, xenobiotic metabolism, TGF-beta signaling, extracellular matrix, cholesterol flux, growth factor receptor signaling, cell trafficking, mouse liver
- MeSH terms
- Animals, Liver, Mass Spectrometry, Proteomics, Mice
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