Mitochondrial Transfer by Photothermal Nanoblade Restores Metabolite Profile in Mammalian Cells
Open accessAbstract
mtDNA sequence alterations are challenging to generate but desirable for basic studies and potential correction of mtDNA diseases. Here, we report a new method for transferring isolated mitochondria into somatic mammalian cells using a photothermal nanoblade, which bypasses endocytosis and cell fusion. The nanoblade rescued the pyrimidine auxotroph phenotype and respiration of ρ0 cells that lack mtDNA. Three stable isogenic nanoblade-rescued clones grown in uridine-free medium showed distinct bioenergetics profiles. Rescue lines 1 and 3 reestablished nucleus-encoded anapleurotic and catapleurotic enzyme gene expression patterns and had metabolite profiles similar to the parent cells from which the ρ0 recipient cells were derived. By contrast, rescue line 2 retained a ρ0 cell metabolic phenotype despite growth in uridine-free selection. The known influence of metabolite levels on cellular processes, including epigenome modifications and gene expression, suggests metabolite profiling can help assess the quality and function of mtDNA-modified cells.
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In vitro transfer of healthy mitochondria to CD4+ T cells from RA patients can restore aspartate metabolism and decrease TNF production compared with untreated controls.
“Moreover, the in vitro transfer of healthy mitochondria to CD4+ T cells from patients with RA can restore aspartate metabolism and decrease the production of TNF, compared with untreated controls286–288.”
From The role of mitochondria in rheumatic diseases (2022) · cites this paper as [286]
The Results sections “Mitochondrial Transfer” and “Metabolite Profiling” (page not provided) report mitochondrial transfer into mtDNA-depleted ρ0 cells and restoration of their overall metabolite profile. They do not report experiments in CD4+ T cells from patients with rheumatoid arthritis, restoration of aspartate metabolism, or decreased TNF production; searches for those terms found no supporting passage. (LLM, conf=0.83)
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References 41 · Cited by 131
- Authors
- Wu, T.-H., Sagullo, E., Case, D., Zheng, X., Li, Y., Hong, J. S., TeSlaa, T., Patananan, A. N., McCaffery, J. M., Niazi, K., Braas, D., Koehler, C. M., Graeber, T. G., Chiou, P.-Y., & Teitell, M. A.
- Affiliations
- University of California, Los Angeles (US)
- Johns Hopkins University (US)
- California NanoSystems Institute (US)
- Corresponding author
- Chiou, Pei-Yu
- Teitell, Michael A.
- ORCID
- Venue
- Cell Metabolism
- Publisher
- Elsevier BV
- Published
- 2016-05
- Volume
- 23
- Issue
- 5
- Pages
- 921-929
- ISSN
- 1550-4131 (ISSN-L)
- PMC
- PMC5062745
- OpenAlex
- W2383250604
- Cited by
- 131
- References
- 41
- Citations by year
- 2026: 15 · 2025: 18 · 2024: 13 · 2023: 14 · 2022: 21 · 2021: 10 · 2020: 12 · 2019: 4 · 2018: 7 · 2017: 12 · 2016: 5
- FWCI
- 4.85
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Cell Metabolism (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Institutes of Health (UL1TR000124)
- National Cancer Institute (R01CA185189, P01CA168585, R01CA090571, R01CA156674, T32CA009120, T32CA009056)
- National Institute of General Medical Sciences (R01GM073981, R01GM114188, T32GM007185, R01GM061721)
- National Institute of Biomedical Imaging and Bioengineering (R21EB014456)
- National Center for Advancing Translational Sciences
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Keywords
- mitochondrial transfer, mitochondrial respiration, metabolite profiling, bioenergetics, mitochondrial biogenesis
- MeSH terms
- Animals, Base Sequence, Clone Cells, DNA, Mitochondrial, Energy Metabolism, Gene Expression Regulation, Humans, Light, Mammals, Mitochondria, Temperature, Reproducibility of Results, Cell Line, Tumor, Nanoparticles, Metabolomics, Metabolome
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