CoChat
Sign Up
Cited by 164Microbial bioremediation and biosurfactantsSurfactants and Colloidal SystemsEnvironmental Chemistry and Analysis

The DOI matches its Crossref record.

Sophorolipids from Candida bombicola using mixed hydrophilic substrates: Production, purification and characterization

Daverey, Achlesh, Pakshirajan, Kannan·2010·Colloids and Surfaces B: Biointerfaces
DOI 10.1016/j.colsurfb.2010.04.002
Open this paper in CoChat — free account

Abstract

Sophorolipids (SLs) are glycolipids type of biosurfactants and are produced by the yeast Candida bombicola. Medium containing mixed hydrophilic substrate (deproteinized whey and glucose), yeast extract and oleic acid was investigated in this study for SLs production from the yeast. The produced SL was also purified and characterized in the study. At an optimum combination of the medium constituents, the yeast C. bombicola produced maximum 23.29+/-0.54 g/l, 25.54+/-1.01 g/l and 33.32+/-0.83 g/l of the biosurfactant when fermentation was carried out in batch shake flasks, in bioreactor without pH control and in bioreactor with pH control, respectively. Produced SL was purified by silica gel column chromatography and was characterized using FTIR, (1)H NMR and LC-MS whose results revealed it to be (17-hydroxyoctadecenoic)-1'4''-lactone-6'6''-diacetate SL. Further, its critical micelle concentration and minimum surface tension against water were found to be 27.17 mg/l and 34.18 mN/m, respectively. Results of interfacial tension obtained using the SLs between water and either n-hexane, sunflower oil or olive oil proved its ability to solubilize non-aqueous phase liquids in water. Further, the biosurfactant was found to be stable at wide range of pHs, temperature and salt concentrations. The results of emulsifying activity and stability of the product against the tested organic solvents and oils together with its ability to solubilize fat and oil confirmed the potential of the biosurfactant in environmental applications.

Overview

Integrity signals

Registry

Crossref · 10.1016/j.colsurfb.2010.04.002

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 29

  1. Fu, 2008, J. Surg. Res., 148, 77, 10.1016/j.jss.2008.03.005 · DOI
  2. M. Maingault, Canada Patent. No. 126,242874 (1997).
  3. Mulligan, 2001, J. Hazard. Mater., 85, 111, 10.1016/S0304-3894(01)00224-2 · DOI
  4. Daverey, 2009, Appl. Biochem. Biotechnol., 158, 663, 10.1007/s12010-008-8449-z · DOI
  5. Daverey, 2009, Food Res. Intern., 42, 499, 10.1016/j.foodres.2009.01.014 · DOI
  6. Spencer, 1970, Antonie Van Leeuwenhoek, 36, 129, 10.1007/BF02069014 · DOI
  7. Gorin, 1961, Can. J. Chem., 39, 846, 10.1139/v61-104 · DOI
  8. Tulloch, 1968, Can. J. Chem., 46, 345, 10.1139/v68-057 · DOI
  9. Konishi, 2008, Science, 57, 359
  10. Daniel, 1998, Biotechnol. Lett., 20, 805, 10.1023/B:BILE.0000015927.29348.1a · DOI
  11. Solaiman, 2007, Biotechnol. Lett., 29, 1341, 10.1007/s10529-007-9407-5 · DOI
  12. Felse, 2007, Enzyme Microb. Technol., 40, 316, 10.1016/j.enzmictec.2006.04.013 · DOI
  13. Rosa, 1998, Int. J. Syst. Bacteriol., 48, 1413, 10.1099/00207713-48-4-1413 · DOI
  14. Asmer, 1988, J. Am. Oil Chem. Soc., 65, 1460, 10.1007/BF02898308 · DOI
  15. Roukas, 2007, Enzyme Microb. Technol., 40, 1578, 10.1016/j.enzmictec.2006.11.003 · DOI
  16. Hu, 2001, Enzyme Microb. Technol., 29, 593, 10.1016/S0141-0229(01)00439-2 · DOI
  17. Cirigliano, 1984, Appl. Environ. Microbiol., 48, 747, 10.1128/AEM.48.4.747-750.1984 · DOI
  18. Urum, 2004, Chemosphere, 57, 1139, 10.1016/j.chemosphere.2004.07.048 · DOI
  19. Van Bogaert, 2007, Appl. Microbiol. Biotechnol., 76, 23, 10.1007/s00253-007-0988-7 · DOI
  20. Casas, 1999, J. Biosci. Bioeng., 88, 488, 10.1016/S1389-1723(00)87664-1 · DOI
  21. Zhou, 1993, Biotechnol. Lett., 15, 477, 10.1007/BF00129322 · DOI
  22. Cooper, 1984, Appl. Environ. Microbiol., 47, 173, 10.1128/AEM.47.1.173-176.1984 · DOI
  23. Zhou, 1992, J. Am. Oil Chem. Soc., 69, 89, 10.1007/BF02635883 · DOI
  24. Zhou, 1995, J. Am. Oil Chem. Soc., 72, 67, 10.1007/BF02635781 · DOI
  25. Daniel, 1999, Appl. Microbiol. Biotechnol., 51, 40, 10.1007/s002530051360 · DOI
  26. Hu, 2001, J. Biotechnol., 87, 263, 10.1016/S0168-1656(01)00248-6 · DOI
  27. Chen, 2006, Enzyme Microb. Technol., 39, 501, 10.1016/j.enzmictec.2005.12.022 · DOI
  28. Otto, 1999, Appl. Microbiol. Biotechnol., 52, 495, 10.1007/s002530051551 · DOI
  29. Ghojavand, 2008, J. Colloid Interface Sci., 324, 172, 10.1016/j.jcis.2008.05.001 · DOI

Cited by

Sorted by citations

Metadata

Authors
Daverey, A., & Pakshirajan, K.
Affiliations
Indian Institute of Technology Guwahati (IN)
Corresponding author
Pakshirajan, Kannan
ORCID
Venue
Colloids and Surfaces B: Biointerfaces
Publisher
Elsevier BV
Published
2010-08
Volume
79
Issue
1
Pages
246-253
ISSN
0927-7765 (ISSN-L)
DOI
10.1016/j.colsurfb.2010.04.002
OpenAlex
W2064675272
Cited by
164
References
0
Citations by year
2026: 5 · 2025: 5 · 2024: 15 · 2023: 10 · 2022: 9 · 2021: 22 · 2020: 20 · 2019: 10 · 2018: 6 · 2017: 12 · 2016: 15 · 2015: 11 · 2014: 7 · 2013: 7 · 2012: 6
FWCI
5.37
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Colloids and Surfaces B Biointerfaces (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Environmental Science › Pollution › Microbial bioremediation and biosurfactants
  • Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Surfactants and Colloidal Systems
  • Physical Sciences › Environmental Science › Environmental Chemistry › Environmental Chemistry and Analysis
Keywords
sophorolipids, Candida bombicola, biosurfactants, glycolipids, deproteinized whey, oleic acid, fermentation medium optimization, biosurfactant purification, critical micelle concentration, interfacial tension, emulsifying activity, emulsion stability, yeast fermentation
MeSH terms
Whey Proteins, Candida, Chromatography, Liquid, Fats, Fermentation, Glucose, Glycolipids, Milk Proteins, Magnetic Resonance Spectroscopy, Oils, Solubility, Mass Spectrometry, Surface Properties, Surface-Active Agents, Yeasts, Spectroscopy, Fourier Transform Infrared, Bioreactors, Oleic Acid, Hydrophobic and Hydrophilic Interactions

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review