Endothelialization of polyurethanes: Surface silanization and immobilization of REDV peptide
Abstract
The paper presents method for chemical immobilization of arginine-glutamic acid-aspartic acid-valine (REDV) peptide on polyurethane surface. The peptide has been covalently bonded using silanes as a spacer molecules. The aim of this work was to investigate the proposed modification process and assess its biological effectiveness, especially in contact with blood and endothelial cells. Physicochemical properties were examined in terms of wettability, atomic composition and density of introduced functional groups and peptide molecules. Experiments with blood showed that material coating reduced number of surface-adhered platelets and fibrinogen molecules. In contrast to polyurethane (PU), there were no blood components deposited on REDV-modified materials (PU-REDV); fibrinogen adsorption on PU-REDV surface has been strongly reduced compared to PU. Analysis of cell adhesion after 1, 2, 3, 4, and 5 days of culture showed a significant increase of the cell-coated area on PU-REDV compared to PU. However, an intense cell growth appeared also on the control surface modified without the addition of REDV. Thus, the positive effect of REDV peptide on the adhesion of HUVEC could not be unequivocally confirmed.
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Fibronectin has a high thrombogenic potential and can initiate the coagulation cascade when the biomaterial coated by fibronectin comes in contact with the patient's blood in vivo.
“Moreover, fibronectin has a high thrombogenic potential and can initiate the coagulation cascade when the biomaterial coated by fibronectin comes in contact with the patient's blood in vivo [233].”
From Liver tissue engineering using decellularized scaffolds: Current progress, challenges, and opportunities (2024) · cites this paper as [233]
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Hussein, K. H., Ahmadzada, B., Correa, J. C., Sultan, A., Wilken, S., Amiot, B., & Nyberg, S. L. (2024) · Bioactive Materials · doi:10.1016/j.bioactmat.2024.06.001 · Cited by 28 · see citing works
“Moreover, fibronectin has a high thrombogenic potential and can initiate the coagulation cascade when the biomaterial coated by fibronectin comes in contact with the patient ’ s blood in vivo [233].”
Background
- Authors
- Butruk-Raszeja, B. A., Dresler, M. S., Kuźmińska, A., & Ciach, T.
- Affiliations
- Warsaw University of Technology (PL)
- Corresponding author
- Butruk-Raszeja, Beata A.
- Venue
- Colloids and Surfaces B: Biointerfaces
- Publisher
- Elsevier BV
- Published
- 2016-08
- Volume
- 144
- Pages
- 335-343
- ISSN
- 0927-7765 (ISSN-L)
- OpenAlex
- W2313492186
- Cited by
- 38
- References
- 0
- Citations by year
- 2025: 3 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 3 · 2020: 5 · 2019: 4 · 2018: 7 · 2017: 3 · 2016: 2
- FWCI
- 2.24
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Colloids and Surfaces B Biointerfaces (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Health Sciences › Medicine › Immunology and Allergy › Cell Adhesion Molecules Research
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Surface Modification and Superhydrophobicity
- Keywords
- polyurethane, endothelialization, REDV peptide, silanization, peptide immobilization, fibrinogen adsorption, platelet adhesion, hemocompatibility, endothelial cell adhesion, human umbilical vein endothelial cells, wettability, surface modification
- MeSH terms
- Adsorption, Adult, Anions, Cations, Cell Adhesion, Female, Fibrinogen, Humans, Oligopeptides, Platelet Adhesiveness, Polyurethanes, Silanes, Surface Properties, Wettability, Cell Proliferation, Immobilized Proteins, Human Umbilical Vein Endothelial Cells
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