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Cited by 19Enzyme Catalysis and ImmobilizationSoil Carbon and Nitrogen DynamicsEnzyme Production and Characterization

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Adsorption of soil invertase to goethite, gibbsite and their organic complexes and the effects on enzyme catalytic performance

Wu, Yue, Zhang, Huihong, Li, Jianwei, Wang, Zhonghua, Jiang, Ying·2023·Colloids and Surfaces B: Biointerfaces
DOI 10.1016/j.colsurfb.2022.113073
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Abstract

Soil invertase plays important role in the enzyme-driven degradation of soil organic carbon, and influenced by the adsorption of minerals or clays. We studied the adsorption effects of goethite, gibbsite and their organic complexes (with tannic acid) on invertase, aiming to explore the influence on the mobility and catalytic behavior of invertase. Results showed that both minerals and mineral-tannic acid complexes (henceforth 'complexes') had considerable adsorption capacity for invertase and restricted its mobility. The catalytic performance of invertase was characterized. Under optimum conditions, the kinetics of the enzyme was reduced markedly after adsorption, indicating the decreased enzyme-substrate affinity. Adsorbed invertases retained 30.9-46.1% of the original activity and the mineral-adsorbed enzymes showed lower activities than the complex-adsorbed enzymes. All minerals and complexes had similar patterns on the influence on the pH and thermal properties of invertase, in which the adsorption improved the pH stability of the enzyme at various pH values, lowered the pH sensitivity and shifted the optimum pH towards the acid sites; while decreased thermal stability at various temperature values and higher temperature sensitivity of the adsorbed invertase were observed. In a 150-h incubation under soil-like conditions, all adsorbed invertases showed greater long-term stability than free invertase, and higher total activities (sum of adsorbed activities and dissolved activities) in adsorption system were observed after the incubation. These results implied that, in a soil microenvironment, the goethite, gibbsite and their organic complexes have a protection effect on invertase through adsorption.

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References 30 · Cited by 19

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Metadata

Authors
Wu, Y., Zhang, H., Li, J., Wang, Z., & Jiang, Y.
Affiliations
Henan Agricultural University (CN)
Corresponding author
Jiang, Ying
ORCID
Venue
Colloids and Surfaces B: Biointerfaces
Publisher
Elsevier BV
Published
2023-02
Volume
222
Pages
113073
ISSN
0927-7765 (ISSN-L)
DOI
10.1016/j.colsurfb.2022.113073
OpenAlex
W4311173392
Cited by
19
References
30
Citations by year
2026: 5 · 2025: 6 · 2024: 7 · 2023: 1
FWCI
1.14
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Colloids and Surfaces B Biointerfaces (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Natural Science Foundation of China (42077050)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme Catalysis and Immobilization
  • Life Sciences › Agricultural and Biological Sciences › Soil Science › Soil Carbon and Nitrogen Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
Keywords
soil invertase, enzyme adsorption, goethite, gibbsite, tannic acid, enzyme activity, enzyme kinetics, pH stability, thermal stability, enzyme protection, enzyme mobility
MeSH terms
Adsorption, Carbon, Hydrogen-Ion Concentration, Minerals, Soil, beta-Fructofuranosidase, Iron Compounds

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CoChat. (2026). Citation check: Adsorption of soil invertase to goethite, gibbsite and their organic complexes and the effects on enzyme catalytic performance. https://app.cochat.ai/papers/10.1016/j.colsurfb.2022.113073
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Wu, Y., Zhang, H., Li, J., Wang, Z., & Jiang, Y. (2023). Adsorption of soil invertase to goethite, gibbsite and their organic complexes and the effects on enzyme catalytic performance. Colloids and Surfaces B: Biointerfaces. https://doi.org/10.1016/j.colsurfb.2022.113073