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Cited by 22Microbial Applications in Construction MaterialsBuilding materials and conservationCalcium Carbonate Crystallization and Inhibition

Synergistic effect of composite bacteria on self-healing process of concrete crack

Open access
Ahmad, Muhammad Arslan, Zhang, Jinlong, Liu, Bing, Guohao, Xie, Xiaoyi, Tan, Haoying, Gu, Changjie, Song, Runhao, Luo, Xiaona, Xie, Weilin, Li, Huang, Rong, Peiwen, Tan, Deng, Xu·2024·Case Studies in Construction Materials
DOI 10.1016/j.cscm.2024.e03028
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Abstract

The calcium precipitation activity (CPA) of bacterial strains can vary in response to shifts in ambient conditions. When these conditions become unsuitable, the CPA of bacterial cells may decrease noticeably, potentially compromising the self-healing process. Nonetheless, the synergistic behavior of various bacterial strains can present additional crystallization sites for calcium precipitation and adapt to diverse ambient conditions, thus enhancing microbial-induced calcium precipitation (MICP). In this article, we delve into the interconnected mechanisms involving forty-one bacterial strains, assessing their initial spore concentration ratios and nutritional needs in relation to CPA. Based on CPA considerations, DSM6307 and B6, were selected and combined. Subsequent investigations were carried out to determine the efficacy of self-healing concrete exposed to co-cultured bacterial strains. The mineralization capacity of these combined strains was bolstered using starch and ammonium nitrate as carbon/nitrogen sources, with the ideal initial spore concentration ratio between B6 and DSM6307 established as 4:6. Through visual assessments confirmed that the synergistic effect of B6-DSM6307 can yield repair efficiencies nearing 100% for crack widths between 0.02 mm and 0.2 mm. Furthermore, water permeability and capillary absorption tests on the sample block demonstrated that the repair compound, derived from the composite spore dry powder B6-DSM6307, effectively seals cracks and…

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Authors
Ahmad, M. A., Zhang, J., Liu, B., Guohao, X., Xiaoyi, T., Haoying, G., Changjie, S., Runhao, L., Xiaona, X., Weilin, L., Huang, R., Peiwen, T., & Deng, X.
Affiliations
  • Shenzhen University (CN)
  • Shenzhen Institute of Information Technology (CN)
ORCID
Venue
Case Studies in Construction Materials
Publisher
Elsevier BV
Published
2024-07
Volume
20
Pages
e03028
ISSN
2214-5095 (ISSN-L)
DOI
10.1016/j.cscm.2024.e03028
OpenAlex
W4392349860
Cited by
22
References
62
Citations by year
2026: 9 · 2025: 8 · 2024: 5
FWCI
2.39
Type
journal article
Language
en
Open access
Licence
Locations
  • Case Studies in Construction Materials (publishedVersion, cc-by-nc-nd)
  • SSRN Electronic Journal (acceptedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Shenzhen University
  • National Natural Science Foundation of China (52178229, 892004042)
Topics
  • Physical Sciences › Environmental Science › Environmental Engineering › Microbial Applications in Construction Materials
  • Physical Sciences › Earth and Planetary Sciences › Earth-Surface Processes › Building materials and conservation
  • Physical Sciences › Materials Science › Biomaterials › Calcium Carbonate Crystallization and Inhibition
Keywords
self-healing concrete, bacterial co-culture, synergistic effect, concrete crack repair, calcite crystal growth, water permeability, capillary water absorption

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