The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Abi, Sra1, and Kette Control the Stability and Localization of SCAR/WAVE to Regulate the Formation of Actin-Based Protrusions
Open accessAbstract
Background In animal cells, GTPase signaling pathways are thought to generate cellular protrusions by modulating the activity of downstream actin-regulatory proteins. Although the molecular events linking activation of a GTPase to the formation of an actin-based process with a characteristic morphology are incompletely understood, Rac-GTP is thought to promote the activation of SCAR/WAVE, whereas Cdc42 is thought to initiate the formation of filopodia through WASP. SCAR and WASP then activate the Arp2/3 complex to nucleate the formation of new actin filaments, which through polymerization exert a protrusive force on the membrane. Results Using RNAi to screen for genes regulating cell form in an adherent Drosophila cell line, we identified a set of genes, including Abi/E3B1, that are absolutely required for the formation of dynamic protrusions. These genes delineate a pathway from Cdc42 and Rac to SCAR and the Arp2/3 complex. Efforts to place Abi in this signaling hierarchy revealed that Abi and two components of a recently identified SCAR complex, Sra1 (p140/PIR121/CYFIP) and Kette (Nap1/Hem), protect SCAR from proteasome-mediated degradation and are critical for SCAR localization and for the generation of Arp2/3-dependent protrusions. Conclusions In Drosophila cells, SCAR is regulated by Abi, Kette, and Sra1, components of a conserved regulatory SCAR complex. By controlling the stability, localization, and function of SCAR, these proteins may help to ensure that Arp2/3 activation and the generation of actin-based protrusions remain strictly dependant on local GTPase signaling.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Indeed, it is reported that reduction of Cyfip1 or fly Hspc300 affects the level of the other components of the WRC (Kunda et al., 2003; Schenck et al., 2004; Abekhoukh and Bardoni, 2014; Gautier et al., 2011; Qurashi et al., 2007; De Rubeis et al., 2013).”
From New insights into the regulatory function of CYFIP1 in the context of WAVE- and FMRP-containing complexes (2017) · cites this paper as Kunda et al., 2003
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Rho GTPases and the Actin Cytoskeleton
Hall, A. (1998) · Science · doi:10.1126/science.279.5350.509 · Cited by 6,138
Cellular Motility Driven by Assembly and Disassembly of Actin Filaments
Pollard, T. D., & Borisy, G. G. (2003) · Cell · doi:10.1016/s0092-8674(03)00120-x · Cited by 4,334
Isolation of a novel gene mutated in Wiskott-Aldrich syndrome
Derry, J. M. J., Ochs, H. D., & Francke, U. (1994) · Cell · doi:10.1016/0092-8674(94)90528-2 · Cited by 1,048
Machesky, L. M., & Insall, R. H. (1998) · Current Biology · doi:10.1016/s0960-9822(98)00015-3 · Cited by 933
Clemens, J. C., Worby, C. A., Simonson-Leff, N., Muda, M., Maehama, T., Hemmings, B. A., & Dixon, J. E. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.110149597 · Cited by 843
Mechanism of regulation of WAVE1-induced actin nucleation by Rac1 and Nck
Eden, S., Rohatgi, R., Podtelejnikov, A. V., Mann, M., & Kirschner, M. W. (2002) · Nature · doi:10.1038/nature00859 · Cited by 835
Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complex
Machesky, L. M., Mullins, R. D., Higgs, H. N., Kaiser, D. A., Blanchoin, L., May, R. C., Hall, M. E., & Pollard, T. D. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.7.3739 · Cited by 773
Autoinhibition and activation mechanisms of the Wiskott–Aldrich syndrome protein
Kim, A. S., Kakalis, L. T., Abdul-Manan, N., Liu, G. A., & Rosen, M. K. (2000) · Nature · doi:10.1038/35004513 · Cited by 765
WAVE, a novel WASP‐family protein involved in actin reorganization induced by Rac
Miki, H., Suetsugu, S., & Takenawa, T. (1998) · The EMBO Journal · doi:10.1093/emboj/17.23.6932 · Cited by 705
Miki, H., Miura, K., & Takenawa, T. (1996) · The EMBO Journal · doi:10.1002/j.1460-2075.1996.tb00917.x · Cited by 672
A functional genomic analysis of cell morphology using RNA interference
Kiger, A. A., Baum, B., Jones, S. J. M., Jones, M. R., Coulson, A. R., Echeverri, C. J., & Perrimon, N. (2003) · Journal of Biology · doi:10.1186/1475-4924-2-27 · Cited by 429
Self-citationMolecular requirements for actin-based lamella formation in Drosophila S2 cells
Rogers, S. L., Wiedemann, U., Stuurman, N., & Vale, R. D. (2003) · The Journal of Cell Biology · doi:10.1083/jcb.200303023 · Cited by 407
Rac function and regulation during Drosophila development
Hakeda‐Suzuki, S., Ng, J., Tzu, J., Dietzl, G., Sun, Y., Harms, M. B., Nardine, T. M., Luo, L., & Dickson, B. J. (2002) · Nature · doi:10.1038/416438a · Cited by 354
Interaction of WASP/Scar proteins with actin and vertebrate Arp2/3 complex
Marchand, J.-B., Kaiser, D. A., Pollard, T. D., & Higgs, H. N. (2001) · Nature Cell Biology · doi:10.1038/35050590 · Cited by 331
Schenck, A., Bardoni, B., Langmann, C., Harden, N., Mandel, J.-L., & Giangrande, A. (2003) · Neuron · doi:10.1016/s0896-6273(03)00354-4 · Cited by 316
Dai, Z., & Pendergast, A. M. (1995) · Genes & Development · doi:10.1101/gad.9.21.2569 · Cited by 271
Regulation of Actin Polymerization by Arp2/3 Complex and WASp/Scar Proteins
Higgs, H. N., & Pollard, T. D. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.46.32531 · Cited by 265
Bear, J. E., Rawls, J. F., & Saxe, C. L. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.142.5.1325 · Cited by 261
SCAR is a primary regulator of Arp2/3-dependent morphological events in Drosophila
Zallen, J. A., Cohen, Y., Hudson, A. M., Cooley, L., Wieschaus, E. F., & Schejter, E. D. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200109057 · Cited by 255
Shi, Y., Ålin, K., & Goff, S. P. (1995) · Genes & Development · doi:10.1101/gad.9.21.2583 · Cited by 247
p140Sra-1 (specifically Rac1-associated protein) is a novel specific target for Rac1 small GTPase
Kobayashi, K., Kuroda, S., Fukata, M., Nakamura, T., Nagase, T., Nomura, N., Matsuura, Y., Yoshida-Kubomura, N., Iwamatsu, A., & Kaibuchi, K. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.1.291 · Cited by 244
The WRP component of the WAVE-1 complex attenuates Rac-mediated signalling
Soderling, S. H., Binns, K. L., Wayman, G. A., Davee, S. M., Ong, S. H., Pawson, T. J., & Scott, J. D. (2002) · Nature Cell Biology · doi:10.1038/ncb886 · Cited by 193
Yanagawa, S.-I., Lee, J.-S., & Ishimoto, A. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.48.32353 · Cited by 173
A subset of dynamic actin rearrangements in Drosophila requires the Arp2/3 complex
Hudson, A. M., & Cooley, L. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200109065 · Cited by 169
PIR121 Regulates Pseudopod Dynamics and SCAR Activity in Dictyostelium
Blagg, S. L., Stewart, M., Sambles, C., & Insall, R. H. (2003) · Current Biology · doi:10.1016/s0960-9822(03)00580-3 · Cited by 150
Stradal, T. E. B., Courtney, K. D., Rottner, K., Hahne, P., Small, J. V., & Pendergast, A. M. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00239-1 · Cited by 148
Ben-Yaacov, S., Borgne, R. L., Abramson, I., Schweisguth, F., & Schejter, E. D. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.152.1.1-b · Cited by 134
N-WASP, WAVE and Mena play different roles in the organization of actin cytoskeleton in lamellipodia
Nakagawa, H., Miki, H., Ito, M., Ohashi, K., Takenawa, T., & Miyamoto, S. (2001) · Journal of Cell Science · doi:10.1242/jcs.114.8.1555 · Cited by 104
Scar/WAVE is localised at the tips of protruding lamellipodia in living cells
Hahne, P., Sechi, A. S., Benesch, S., & Small, J. V. (2001) · FEBS Letters · doi:10.1016/s0014-5793(01)02239-6 · Cited by 103
Kette regulates actin dynamics and genetically interacts with Wave and Wasp
Bogdan, S., & Klämbt, C. (2003) · Development · doi:10.1242/dev.00663 · Cited by 89
Juang, J., & Hoffmann, F. M. (1999) · Oncogene · doi:10.1038/sj.onc.1202911 · Cited by 66
Suzuki, T., Nishiyama, K., Yamamoto, A., Inazawa, J., Iwaki, T., Yamada, T., Kanazawa, I., & Sakaki, Y. (2000) · Genomics · doi:10.1006/geno.1999.6053 · Cited by 66
Molecular Cloning of p125Nap1, a Protein That Associates with an SH3 Domain of Nck
Kitamura, T., Kitamura, Y., Kazuyoshi, Totty, N. F., Gout, I. T., Hara, K., Waterfield, M. D., Sakaue, M., Ogawa, W., & Kasuga, M. (1996) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1996.0264 · Cited by 62
Interaction of Nck-associated protein 1 with activated GTP-binding protein Rac
Kitamura, Y., Kitamura, T., Sakaue, H., Maeda, T., Ueno, H., Nishio, S., Ohno, S., Osada, S., Sakaue, M., Ogawa, W., et al. (1997) · Biochemical Journal · doi:10.1042/bj3220873 · Cited by 53
Yamamoto, A., Suzuki, T., & Sakaki, Y. (2001) · Gene · doi:10.1016/s0378-1119(01)00521-2 · Cited by 38
Cited by
Sorted by citations
Actin cytoskeleton vulnerability to disulfide stress mediates disulfidptosis
Liu, X., Nie, L., Zhang, Y., Yan, Y., Wang, C., Colic, M., Olszewski, K. L., Horbath, A. D., Chen, X., Lei, G., et al. (2023) · Nature Cell Biology · doi:10.1038/s41556-023-01091-2 · Cited by 1,212
Regulation of Actin Filament Assembly by Arp2/3 Complex and Formins
Pollard, T. D. (2007) · Annual Review of Biophysics and Biomolecular Structure · doi:10.1146/annurev.biophys.35.040405.101936 · Cited by 1,064
The role of the Rho GTPases in neuronal developmentPreprint
Govek, E., Newey, S. E., & Aelst, L. V. (2005) · Genes & Development · doi:10.1101/gad.1256405 · Cited by 1,030
The WASP-WAVE protein network: Connecting the membrane to the cytoskeleton.
Takenawa, T., & Suetsugu, S. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2069 · Cited by 947
The Fragile X Syndrome Protein Represses Activity-Dependent Translation through CYFIP1, a New 4E-BP
Napoli, I., Mercaldo, V., Boyl, P. P., Eleuteri, B., Zalfa, F., De Rubeis, S., Di Marino, D., Mohr, E., Massimi, M., Falconi, M., et al. (2008) · Cell · doi:10.1016/j.cell.2008.07.031 · Cited by 609
Metadata
- Authors
- Kunda, P., Craig, G., Dominguez, V., & Baum, B.
- Affiliations
- University College London (GB)
- Corresponding author
- Baum, Buzz
- ORCID
- Baum, Buzz
- Venue
- Current Biology
- Publisher
- Elsevier BV
- Published
- 2003-10
- Volume
- 13
- Issue
- 21
- Pages
- 1867-1875
- ISSN
- 0960-9822 (ISSN-L)
- OpenAlex
- W2054821269
- Cited by
- 341
- References
- 35
- Citations by year
- 2026: 1 · 2025: 6 · 2024: 11 · 2023: 8 · 2022: 10 · 2021: 12 · 2020: 9 · 2019: 11 · 2018: 15 · 2017: 13 · 2016: 13 · 2015: 12 · 2014: 12 · 2013: 21 · 2012: 10
- FWCI
- 7.59
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 3577 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Current Biology (publishedVersion)
- PubMed (publishedVersion)
- UCL Discovery (University College London) (submittedVersion)
- Funders
- Cancer Research UK
- Ludwig Institute for Cancer Research
- Royal Society
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Keywords
- SCAR/WAVE complex, ABI, SR-A1, Arp2/3 complex, actin-based protrusions, filopodia, Cdc42, Rac, GTPase signaling, proteasomal degradation, protein localization, Drosophila cells, cell shape regulation, wasps
- MeSH terms
- Actins, Animals, Carrier Proteins, Cells, Cultured, Drosophila, Fluorescent Antibody Technique, Microfilament Proteins, Pseudopodia, Blotting, Western, Signal Transduction, Drosophila Proteins, RNA Interference, Adaptor Proteins, Signal Transducing, Wiskott-Aldrich Syndrome Protein Family
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
